Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type OpdA and mutants in Escherichia coli strain DH5alpha | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Y607A | the mutant shows altered substrate and cleavage site specificity compared to the wild-type enzyme, structure comparison with the wild-type enzyme, overview. The mutant is highly activated by NaCl in contrast to the wild-type enzyme | Escherichia coli |
Y607F | the mutant shows altered substrate and cleavage site specificity compared to the wild-type enzyme, structure comparison with the wild-type enzyme, overview. The mutant is highly activated by NaCl in contrast to the wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes, overview | Escherichia coli | |
0.0002 | - |
Abz-GRSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0004 | - |
Abz-GHSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0004 | - |
Abz-GKSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0005 | - |
Abz-GSSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0007 | - |
Abz-GFSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0007 | - |
Abz-GPSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0007 | - |
Abz-GYSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0008 | - |
Abz-GASPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0011 | - |
Abz-GGSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0018 | - |
Abz-GESPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0018 | - |
Abz-GLSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0018 | - |
Abz-GNSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0022 | - |
Abz-GQSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0042 | - |
Abz-GDSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dependent on | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type OpdA and mutants from Escherichia coli strain DH5alpha by nickel affinity chromatography | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
hydrolysis of oligopeptides, with broad specificity. Gly or Ala commonly occur as P1 or P1' residues, but more distant residues are also important, as is shown by the fact that Z-Gly-Pro-Gly-/-Gly-Pro-Ala is cleaved, but not Z-(Gly)5 | the Tyr607 residue is involved in the recognition of the substrate with a key role in catalysis, it plays an important role in the enzyme-substrate interaction and in the hydrolytic activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-GASPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GA + SPFRQ-EDDnp | - |
? | |
Abz-GDSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GD + SPFRQ-EDDnp | - |
? | |
Abz-GESPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GE + SPFRQ-EDDnp | - |
? | |
Abz-GFSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GF + SPFRQ-EDDnp | - |
? | |
Abz-GGSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GG + SPFRQ-EDDnp | - |
? | |
Abz-GHSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GH + SPFRQ-EDDnp | - |
? | |
Abz-GKSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GK + SPFRQ-EDDnp | - |
? | |
Abz-GLSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GL + SPFRQ-EDDnp | - |
? | |
Abz-GNSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GN + SPFRQ-EDDnp | - |
? | |
Abz-GPSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GP + SPFRQ-EDDnp | - |
? | |
Abz-GQSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GQ + SPFRQ-EDDnp | - |
? | |
Abz-GRSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GR + SPFRQ-EDDnp | - |
? | |
Abz-GSSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GS + SPFRQ-EDDnp | - |
? | |
Abz-GYSPFRQ-EDDnp + H2O | - |
Escherichia coli | Abz-GY + SPFRQ-EDDnp | - |
? | |
additional information | substrate specificities and cleavage sites of OpdA mutants, overview | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | in OpdA, the flexible loop is formed by residues 600-614, 600SHIFAGGYAAGYYSY614, modeling studies, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the M3 peptidase family with four conserved Tyr residues on a flexible loop in close proximity to the catalytic site | Escherichia coli |
oligopeptidase A | - |
Escherichia coli |
OpdA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
Abz-GESPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.1 | - |
Abz-GPSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.2 | - |
Abz-GDSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.2 | - |
Abz-GGSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.3 | - |
Abz-GSSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.4 | - |
Abz-GKSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.5 | - |
Abz-GASPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.5 | - |
Abz-GNSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.6 | - |
Abz-GQSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.74 | - |
Abz-GHSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
1.1 | - |
Abz-GFSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
1.5 | - |
Abz-GLSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
1.5 | - |
Abz-GYSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
2.4 | - |
Abz-GRSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | activity range, profile, overview | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
42 | - |
Abz-GESPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
49 | - |
Abz-GDSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
159 | - |
Abz-GPSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
194 | - |
Abz-GGSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
258 | - |
Abz-GQSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
273 | - |
Abz-GNSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
590 | - |
Abz-GSSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
595 | - |
Abz-GASPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
844 | - |
Abz-GLSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
1095 | - |
Abz-GKSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
1584 | - |
Abz-GFSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
1920 | - |
Abz-GHSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
2191 | - |
Abz-GYSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli | |
10360 | - |
Abz-GRSPFRQ-EDDnp | pH 7.4, 37°C, recombinant wild-type enzyme | Escherichia coli |