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Literature summary for 3.4.24.70 extracted from

  • Lorenzon, R.Z.; Cunha, C.E.; Marcondes, M.F.; Machado, M.F.; Juliano, M.A.; Oliveira, V.; Travassos, L.R.; Paschoalin, T.; Carmona, A.K.
    Kinetic characterization of the Escherichia coli oligopeptidase A (OpdA) and the role of the Tyr(607) residue (2010), Arch. Biochem. Biophys., 500, 131-136.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type OpdA and mutants in Escherichia coli strain DH5alpha Escherichia coli

Protein Variants

Protein Variants Comment Organism
Y607A the mutant shows altered substrate and cleavage site specificity compared to the wild-type enzyme, structure comparison with the wild-type enzyme, overview. The mutant is highly activated by NaCl in contrast to the wild-type enzyme Escherichia coli
Y607F the mutant shows altered substrate and cleavage site specificity compared to the wild-type enzyme, structure comparison with the wild-type enzyme, overview. The mutant is highly activated by NaCl in contrast to the wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of wild-type and mutant enzymes, overview Escherichia coli
0.0002
-
Abz-GRSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0004
-
Abz-GHSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0004
-
Abz-GKSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0005
-
Abz-GSSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0007
-
Abz-GFSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0007
-
Abz-GPSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0007
-
Abz-GYSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0008
-
Abz-GASPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0011
-
Abz-GGSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0018
-
Abz-GESPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0018
-
Abz-GLSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0018
-
Abz-GNSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0022
-
Abz-GQSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.0042
-
Abz-GDSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type OpdA and mutants from Escherichia coli strain DH5alpha by nickel affinity chromatography Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
hydrolysis of oligopeptides, with broad specificity. Gly or Ala commonly occur as P1 or P1' residues, but more distant residues are also important, as is shown by the fact that Z-Gly-Pro-Gly-/-Gly-Pro-Ala is cleaved, but not Z-(Gly)5 the Tyr607 residue is involved in the recognition of the substrate with a key role in catalysis, it plays an important role in the enzyme-substrate interaction and in the hydrolytic activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-GASPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GA + SPFRQ-EDDnp
-
?
Abz-GDSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GD + SPFRQ-EDDnp
-
?
Abz-GESPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GE + SPFRQ-EDDnp
-
?
Abz-GFSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GF + SPFRQ-EDDnp
-
?
Abz-GGSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GG + SPFRQ-EDDnp
-
?
Abz-GHSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GH + SPFRQ-EDDnp
-
?
Abz-GKSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GK + SPFRQ-EDDnp
-
?
Abz-GLSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GL + SPFRQ-EDDnp
-
?
Abz-GNSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GN + SPFRQ-EDDnp
-
?
Abz-GPSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GP + SPFRQ-EDDnp
-
?
Abz-GQSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GQ + SPFRQ-EDDnp
-
?
Abz-GRSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GR + SPFRQ-EDDnp
-
?
Abz-GSSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GS + SPFRQ-EDDnp
-
?
Abz-GYSPFRQ-EDDnp + H2O
-
Escherichia coli Abz-GY + SPFRQ-EDDnp
-
?
additional information substrate specificities and cleavage sites of OpdA mutants, overview Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
More in OpdA, the flexible loop is formed by residues 600-614, 600SHIFAGGYAAGYYSY614, modeling studies, overview Escherichia coli

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the M3 peptidase family with four conserved Tyr residues on a flexible loop in close proximity to the catalytic site Escherichia coli
oligopeptidase A
-
Escherichia coli
OpdA
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
Abz-GESPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.1
-
Abz-GPSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.2
-
Abz-GDSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.2
-
Abz-GGSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.3
-
Abz-GSSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.4
-
Abz-GKSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.5
-
Abz-GASPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.5
-
Abz-GNSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.6
-
Abz-GQSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
0.74
-
Abz-GHSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
1.1
-
Abz-GFSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
1.5
-
Abz-GLSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
1.5
-
Abz-GYSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
2.4
-
Abz-GRSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 activity range, profile, overview Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
42
-
Abz-GESPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
49
-
Abz-GDSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
159
-
Abz-GPSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
194
-
Abz-GGSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
258
-
Abz-GQSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
273
-
Abz-GNSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
590
-
Abz-GSSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
595
-
Abz-GASPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
844
-
Abz-GLSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
1095
-
Abz-GKSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
1584
-
Abz-GFSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
1920
-
Abz-GHSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
2191
-
Abz-GYSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli
10360
-
Abz-GRSPFRQ-EDDnp pH 7.4, 37°C, recombinant wild-type enzyme Escherichia coli