3.4.24.70: oligopeptidase A
This is an abbreviated version!
For detailed information about oligopeptidase A, go to the full flat file.
Word Map on EC 3.4.24.70
-
3.4.24.70
-
prolyl
-
typhimurium
-
dipeptidyl
-
carboxypeptidase
-
neuropeptides
-
endopeptidases
-
oxyanion
-
subtilisin
-
octapeptide
-
post-proline
-
thimet
-
neurolysin
-
z-pro-prolinal
-
bell-shaped
-
product-like
-
scissile
-
3.4.24.15
-
arrhenius
-
metallopeptidase
-
bradykinin
- 3.4.24.70
-
prolyl
- typhimurium
-
dipeptidyl
- carboxypeptidase
- neuropeptides
- endopeptidases
-
oxyanion
- subtilisin
- octapeptide
-
post-proline
-
thimet
- neurolysin
- z-pro-prolinal
-
bell-shaped
-
product-like
-
scissile
-
3.4.24.15
-
arrhenius
- metallopeptidase
- bradykinin
Reaction
hydrolysis of oligopeptides, with broad specificity. Gly or Ala commonly occur as P1 or P1' residues, but additional information distant residues are also important, as is shown by the fact that Z-Gly-Pro-Gly-/-Gly-Pro-Ala is cleaved, but not Z-(Gly)5 =
Synonyms
68000-M Signalpeptide hydrolase, More, oligopeptidase A, OpdA, prlC, TOP, Top1, Top2
ECTree
3.4.24.70 oligopeptidase AAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.4.24.70 - oligopeptidase A
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-casein-derived peptides + H2O
?
-
alpha-casein is not cleaved by OpdA, but by proteases HslUV, ClpAP, or Lon, the peptide products are then cleaved by OpdA, overview
-
-
?
bacteriophage P22 gp7 protein
?
-
-
-
-
?
Benzyloxycarbonyl-Ala-Ala-Leu 4-nitroanilide + H2O
Benzyloxycarbonyl-Ala-Ala + Leu 4-nitroanilide
Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala + H2O
Benzyloxycarbonyl-Gly-Pro-Gly + Gly-Pro-Ala
o-aminobenzoyl-NKPRRPQ-N-(2,4-dinitrophenyl)-ethylenediamine
?
-
best substrate for OpdA
-
-
?
Acetyl-Ala-Ala-Ala-Ala + H2O
Ala-Ala-Ala + acetyl-Ala
-
-
-
?
Acetyl-Ala-Ala-Ala-Ala + H2O
Ala-Ala-Ala + acetyl-Ala
-
-
-
-
?
Bacteriophage P22 gp7 protein + H2O
?
-
-
-
-
?
Bacteriophage P22 gp7 protein + H2O
?
-
directly or indirectly involved in post-translational processing, required for normal growth of phage P22
-
-
?
Benzyloxycarbonyl-Ala-Ala-Leu 4-nitroanilide + H2O
Benzyloxycarbonyl-Ala-Ala + Leu 4-nitroanilide
-
-
-
?
Benzyloxycarbonyl-Ala-Ala-Leu 4-nitroanilide + H2O
Benzyloxycarbonyl-Ala-Ala + Leu 4-nitroanilide
-
-
-
?
Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala + H2O
Benzyloxycarbonyl-Gly-Pro-Gly + Gly-Pro-Ala
-
-
-
?
Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala + H2O
Benzyloxycarbonyl-Gly-Pro-Gly + Gly-Pro-Ala
-
-
-
?
o-aminobenzoyl-GFSIFRQ-N-(2,4-dinitrophenyl)-ethylenediamine
?
-
-
-
-
?
o-aminobenzoyl-GFSPFR-N-(2,4-dinitrophenyl)-ethylenediamine
?
-
-
-
-
?
Prolipoprotein signal peptide + H2O
?
-
further degrades partially degraded portions of signal peptide yielded by protease IV
-
-
?
Prolipoprotein signal peptide + H2O
?
-
in vitro studies suggest oligopeptidase A may be involved in degradation of signal peptides
-
-
?
Prolipoprotein signal peptide + H2O
?
-
in vitro studies suggest oligopeptidase A may be involved in degradation of signal peptides
-
-
?
Prolipoprotein signal peptide + H2O
Hydrolyzed prolipoprotein signal peptide
-
-
smallest fragment: tripeptide
?
Prolipoprotein signal peptide + H2O
Hydrolyzed prolipoprotein signal peptide
-
proposed cleavage sites
peptide fragments identified
?
Prolipoprotein signal peptide + H2O
Hydrolyzed prolipoprotein signal peptide
-
methionine-labelled
peptide fragments identified
?
Prolipoprotein signal peptide + H2O
Hydrolyzed prolipoprotein signal peptide
-
-
smallest fragment: tripeptide
?
additional information
?
-
-
benzyloxycarbonyl-Gly-Gly-Gly-Gly or benzyloxycarbonyl-Gly-Gly-Gly-Gly-Gly
-
-
?
additional information
?
-
-
substrates are N-blocked peptides with at least 4 amino acid residues or unblocked pentapeptides, no substrates are signal peptide still attached to the mature protein
-
-
?
additional information
?
-
-
no cleavage of Abz-GFPPFRQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
-
?
additional information
?
-
-
substrate specificities and cleavage sites of OpdA mutants, overview
-
-
?
additional information
?
-
-
no cleavage of Abz-GFPPFRQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
-
?
additional information
?
-
-
the enzyme digests proteins to short peptides that are subsequently hydrolyzed to smaller fragments and free amino acids, protein degradation is an essential quality control and regulatory function
-
-
?
additional information
?
-
-
the enzyme cleaves peptides generated by the activity of the three primary ATP-dependent proteases Lon, HslUV, and ClpAP from Escherichia coli, overview
-
-
?
additional information
?
-
-
benzyloxycarbonyl-Gly-Gly-Gly-Gly or benzyloxycarbonyl-Gly-Gly-Gly-Gly-Gly
-
-
?
additional information
?
-
-
benzyloxycarbonyl-Gly-Gly-Gly-Gly or benzyloxycarbonyl-Gly-Gly-Gly-Gly-Gly
-
-
?
additional information
?
-
-
hydrolyzes N-blocked peptides containing at least four amino acids but is unable to hydrolyze unblocked peptides with fewer than five amino acids, Gly or Ala on either side of the scissile bond is permissive for hydrolysis
-
-
?
