3.4.24.66: choriolysin L
This is an abbreviated version!
For detailed information about choriolysin L, go to the full flat file.
Word Map on EC 3.4.24.66
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3.4.24.66
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envelope
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oryzias
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latipes
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zebrafish
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swell
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whole-mount
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rerio
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fundulus
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heteroclitus
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exon-intron
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astacin
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teleostean
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euteleosts
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oviparous
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choriogenins
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salmon
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ovoviviparous
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masou
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astacin-like
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hypoblast
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three-spined
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stickleback
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salar
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brachydanio
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rockfish
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coregonus
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brood
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molecular biology
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degradation
- 3.4.24.66
- envelope
- oryzias
- latipes
- zebrafish
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swell
-
whole-mount
- rerio
- fundulus
- heteroclitus
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exon-intron
- astacin
-
teleostean
-
euteleosts
-
oviparous
-
choriogenins
- salmon
-
ovoviviparous
- masou
-
astacin-like
-
hypoblast
-
three-spined
- stickleback
- salar
-
brachydanio
- rockfish
-
coregonus
-
brood
- molecular biology
- degradation
Reaction
Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-/-7-(4-methyl)coumarylamide =
Synonyms
choriolysin L, chorionase, EHE, embryo-specific hatching enzyme, FLCE, LCE, Low choriolytic enzyme, medaka hatching enzyme, medaka low choriolytic enzyme, MLCE, More, Teleost hatching enzyme (component)
ECTree
3.4.24.66 choriolysin LAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.4.24.66 - choriolysin L
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
?
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hydrolysis at about 8% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
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?
EVLPLDNPPPA + H2O
EVLPLD + NPPPA
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specific activity: 8.66 nmol/min/microgram protein
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?
EVQPPDSPLSI + H2O
EVQPPD + SPLSI
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location: D387/S388 in ChgL (ZI-1,2). Specific activity: 136 nmol/30min/microgram enzyme
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?
PGKNPNTPPIG + H2O
PGKNPN + TPPIG
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location: N220/T221 in ChgH (ZI-1,2). Specific activity: 23.9 nmol/30min/microgram enzyme
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?
PKLFQDGKPSN + H2O
PKLFQ + DGKPSN
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location: Q136/D137 in ChgH (ZI-1,2). Specific activity: 0.2 nmol/30min/microgram enzyme
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?
PSKRPEAPGVP + H2O
PSKRPE + APGVP
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specific activity: 0.56 nmol/min/microgram protein
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?
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
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best substrate of peptidyl 4-methylcoumarin 7-amides
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?
SVPVVRTSQAA + H2O
SVPVVR + TSQAA
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specific activity: 19.5 nmol/min/microgram protein
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?
VPFELRYPVPA + H2O
VPFELR + YPVPA
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specific activity: 10.36 nmol/min/microgram protein
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?
VPFEQRYPVPA + H2O
VPFEQR + YPVPA
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location: R73/Y74 in ChgL (ZI-3). Specific activity: 76.3 nmol/30min/microgram enzyme
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?
YPSKPQTPTET + H2O
YPSKPQ + TPTET
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specific activity: 2.71 nmol/min/microgram protein
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?
Swollen chorion + H2O
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i.e. inner layer of chorion that has been swollen by previous action of choriolysin H, EC 3.4.24.67, essential reaction in choriolysis, component of embryo-secreted hatching enzyme that digests egg envelope
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?
additional information
?
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egg envelope digestion: by electron microscopy it is shown that the egg envelope becomes swollen after treatment with the purified EHE. The EHE cleavage sites on the zona pellucida (ZP) protein of the egg envelope are located in the N-terminal repeat regions
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?
additional information
?
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cleavage sites of FHCE and FLCE on egg envelope subunit proteins are determined by comparing the N-terminal amino acid sequences of digests with the sequences deduced from the cDNAs for egg envelope subunit proteins. FHCE and FLCE cleave different sites of the subunit proteins. FLCE cleaves the inside of the zona pellucida domain, the core structure of egg envelope subunit protein, to completely digest the FHCE swollen envelope
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?
additional information
?
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unfertilized egg envelope (UFE) is digested by one of the enzymes FHCE1/2 or FLCE
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?
additional information
?
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poor substrates are succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide, succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Leu-Ser-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Leu-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Val-Leu-Lys 4-methylcoumarin 7-amide, no substrates are intact chorion or Gly-Pro 4-methylcoumarin 7-amide
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?
additional information
?
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cleavage sites of HCE and LCE on the egg envelope that are primarily constructed of two groups of subunit proteins, ZI-1,2 and ZI-3, are determined. LCE cleaves the middle of the zona pellucida (ZP) domain of ZI-1,2, in addition to the upstream of the trefoil domain of ZI-1,2 and the ZP domain of ZI-3. This middle site is in the intervening sequence connecting two subdomains of the ZP domain. Cleaving this site results in the solubilization of the swollen egg envelope by the disruption of the filamentous structure that is formed by the non-covalent polymerization of ZP domains
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?
