3.4.24.66: choriolysin L

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For detailed information about choriolysin L, go to the full flat file.

Reaction

Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-/-7-(4-methyl)coumarylamide =

Synonyms

choriolysin L, chorionase, EHE, embryo-specific hatching enzyme, FLCE, LCE, Low choriolytic enzyme, medaka hatching enzyme, medaka low choriolytic enzyme, MLCE, More, Teleost hatching enzyme (component)

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.66 choriolysin L

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Results	in table
1	AA Sequence
2	Application
1	CAS Registry Number
6	Cloned(Commentary)
1	Expression
2	General Information
4	Inhibitors
1	KM Value [mM]
3	Localization
8	Metals/Ions
4	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
12	Organism
5	pH Optimum
1	pH Range
5	Purification (Commentary)
1	Reaction
1	Reaction Type
12	Reference
18	Source Tissue
1	Specific Activity [micromol/min/mg]
32	Substrates and Products (Substrate)
2	Subunits
23	Synonyms
2	Temperature Optimum [°C]

Natural Substrates Products

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Natural Substrates Products on EC 3.4.24.66 - choriolysin L

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

Swollen chorion + H2O

?

Oryzias latipes

-

i.e. inner layer of chorion that has been swollen by previous action of choriolysin H, EC 3.4.24.67, essential reaction in choriolysis, component of embryo-secreted hatching enzyme that digests egg envelope

31413

-

-

?

additional information

?

-

Anguilla japonica

-

egg envelope digestion: by electron microscopy it is shown that the egg envelope becomes swollen after treatment with the purified EHE. The EHE cleavage sites on the zona pellucida (ZP) protein of the egg envelope are located in the N-terminal repeat regions

719462

-

-

?