Information on EC 3.4.24.66 - choriolysin L

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The expected taxonomic range for this enzyme is: Elopocephala

EC NUMBER
COMMENTARY
3.4.24.66
-
RECOMMENDED NAME
GeneOntology No.
choriolysin L
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-/-7-(4-methyl)coumarylamide
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of peptide bond
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-
-
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
choriolysin L
-
-
chorionase
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EHE
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contains several isoenzymes
embryo-specific hatching enzyme
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Low choriolytic enzyme
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-
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Low choriolytic enzyme
-
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Low choriolytic enzyme
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medaka hatching enzyme
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medaka low choriolytic enzyme
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-
Teleost hatching enzyme (component)
-
-
-
-
additional information
-
homologue of astacin
additional information
-
the enzyme is an astacin family zinc endopeptidase
CAS REGISTRY NUMBER
COMMENTARY
177529-15-6
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
medaka, teleost fish, orange-red variety
-
-
Manually annotated by BRENDA team
orange-red variety of medaka
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
hydrolysis at about 8% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
-
casein + H2O
?
show the reaction diagram
-
-
-
-
-
casein + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
chorion + H2O
?
show the reaction diagram
-
-
-
-
?
chorion + H2O
?
show the reaction diagram
-
synergetic digestion by HCE and LCE
-
-
?
EVLPLDNPPPA + H2O
EVLPLD + NPPPA
show the reaction diagram
-
specific activity: 8.66 nmol/min/microgram protein
-
-
?
EVQPPDSPLSI + H2O
EVQPPD + SPLSI
show the reaction diagram
-
location: D387/S388 in ChgL (ZI-1,2). Specific activity: 136 nmol/30min/microgram enzyme
-
-
?
PGKNPNTPPIG + H2O
PGKNPN + TPPIG
show the reaction diagram
-
location: N220/T221 in ChgH (ZI-1,2). Specific activity: 23.9 nmol/30min/microgram enzyme
-
-
?
PKLFQDGKPSN + H2O
PKLFQ + DGKPSN
show the reaction diagram
-
location: Q136/D137 in ChgH (ZI-1,2). Specific activity: 0.2 nmol/30min/microgram enzyme
-
-
?
PSKRPEAPGVP + H2O
PSKRPE + APGVP
show the reaction diagram
-
specific activity: 0.56 nmol/min/microgram protein
-
-
?
RPTFGRPGIT + H2O
RPTFG + RPGIT
show the reaction diagram
-
-
-
-
?
RPTIGRPGFT + H2O
RPTIG + RPGFT
show the reaction diagram
-
-
-
-
?
RPTLGEPTHT + H2O
RPTLG + EPTHT
show the reaction diagram
-
-
-
-
?
RPTLGGPKGS + H2O
RPTLG + GPKGS
show the reaction diagram
-
-
-
-
?
Suc-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
best substrate of peptidyl 4-methylcoumarin 7-amides
-
-
-
SVPVVRTSQAA + H2O
SVPVVR + TSQAA
show the reaction diagram
-
specific activity: 19.5 nmol/min/microgram protein
-
-
?
Swollen chorion + H2O
?
show the reaction diagram
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-
-
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Swollen chorion + H2O
?
show the reaction diagram
-
i.e. partially digested chorion, i.e. inner layer of chorion that has been swollen by previous action of choriolysin H, EC 3.4.24.67, essential reaction in choriolysis, component of embryo-secreted hatching enzyme that digests egg envelope
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VPFELRYPVPA + H2O
VPFELR + YPVPA
show the reaction diagram
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specific activity: 10.36 nmol/min/microgram protein
-
-
?
VPFEQRYPVPA + H2O
VPFEQR + YPVPA
show the reaction diagram
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location: R73/Y74 in ChgL (ZI-3). Specific activity: 76.3 nmol/30min/microgram enzyme
-
-
?
YPSKPQTPTET + H2O
YPSKPQ + TPTET
show the reaction diagram
-
specific activity: 2.71 nmol/min/microgram protein
-
-
?
GSKGSDETFH + H2O
GSKGS + DETFH
show the reaction diagram
-
-
-
-
?
additional information
?
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-
-
-
-
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additional information
?
-
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poor substrates are succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide, succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Leu-Ser-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Leu-Thr-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide, N-tert-butoxycarbonyl-Val-Leu-Lys 4-methylcoumarin 7-amide, no substrates are intact chorion or Gly-Pro 4-methylcoumarin 7-amide
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additional information
?
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egg envelope digestion: by electron microscopy it is shown that the egg envelope becomes swollen after treatment with the purified EHE. The EHE cleavage sites on the zona pellucida (ZP) protein of the egg envelope are located in the N-terminal repeat regions
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-
-
additional information
?
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cleavage sites of FHCE and FLCE on egg envelope subunit proteins are determined by comparing the N-terminal amino acid sequences of digests with the sequences deduced from the cDNAs for egg envelope subunit proteins. FHCE and FLCE cleave different sites of the subunit proteins. FLCE cleaves the inside of the zona pellucida domain, the core structure of egg envelope subunit protein, to completely digest the FHCE swollen envelope
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additional information
?
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cleavage sites of HCE and LCE on the egg envelope that are primarily constructed of two groups of subunit proteins, ZI-1,2 and ZI-3, are determined. LCE cleaves the middle of the zona pellucida (ZP) domain of ZI-1,2, in addition to the upstream of the trefoil domain of ZI-1,2 and the ZP domain of ZI-3. This middle site is in the intervening sequence connecting two subdomains of the ZP domain. Cleaving this site results in the solubilization of the swollen egg envelope by the disruption of the filamentous structure that is formed by the non-covalent polymerization of ZP domains
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-
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additional information
?
-
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unfertilized egg envelope (UFE) is digested by one of the enzymes FHCE1/2 or FLCE
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Swollen chorion + H2O
?
show the reaction diagram
-
i.e. inner layer of chorion that has been swollen by previous action of choriolysin H, EC 3.4.24.67, essential reaction in choriolysis, component of embryo-secreted hatching enzyme that digests egg envelope
-
-
-
additional information
?
-
-
egg envelope digestion: by electron microscopy it is shown that the egg envelope becomes swollen after treatment with the purified EHE. The EHE cleavage sites on the zona pellucida (ZP) protein of the egg envelope are located in the N-terminal repeat regions
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-
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Calcium
-
0.00538 mg/mg protein, inductively coupled plasma emission analysis
Cu2+
-
partly reactivates EDTA-inactivated apoenzyme
Zinc
-
0.00124 mg/mg protein, inductively coupled plasma emission analysis
Zinc
-
Zn-binding motif
Zn2+
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reactivates EDTA-inactivated apoenzyme
Zn2+
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zinc endopeptidase
Magnesium
-
0.00164 mg/mg protein, inductively coupled plasma emission analysis
additional information
-
no cobalt, manganese or iron detected by inductively coupled plasma emission analysis
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
-
0.5 mM, choriolytic and caseinolytic activity, 1 mM Zn2+ restores, 5 mM Cu2+ partially restores, not Ca2+ or Mg2+
EDTA
-
inhibits at 1 mM
additional information
-
no inhibition of caseinolytic activity by 1-10 mM IAA, monoiodoacetic acid or diisopropyl fluorophosphate
-
additional information
-
not inhibited by DFP, iodoacetamide or iodoacetic acid
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KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
48
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EVQPPDSPLSI
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pH 8.0, 30°C
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SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
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-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8
-
-
assay at
8
-
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assay at
8.5
-
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caseinolytic activity
8.6
-
-
for caseinolytic activity
additional information
-
-
pI: 9.8
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
9
-
about 50% of maximal caseinolytic activity at pH 6.5 and 8.9
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at
30
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
in stage 25 embryos, strong expression of LCE found in branchial arches
Manually annotated by BRENDA team
-
prehatching embryo, 3 days old, embryo-secreted enzyme
Manually annotated by BRENDA team
-
the enzyme is expressed only in pre-hatching embryos
Manually annotated by BRENDA team
-
in stage 19 embryos, LCE gene transcripts detected in a U-shaped cell mass at the anterior of the forebrain
Manually annotated by BRENDA team
-
colocalized with choriolysin H in the secretory granules
Manually annotated by BRENDA team
-
i.e. culture medium of blastulae; taken immediately after larvae hatched on the 6th day
Manually annotated by BRENDA team
-
i.e. culture medium of blastulae
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
at the periphery, immunocytochemical detection
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
23100
-
-
Oryzias latipes, deduced from nucleotide sequence
25500
-
-
-
additional information
-
-
amino acid composition
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
1 * 25500, Oryzias latipes, SDS-PAGE
monomer
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-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
from hatching liquid
-
partly purified by gel filtration and cation-exchange column
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
inserted into a pGEM-T easy vector, cloning of full-length c-DNA by 5'- and 3'-RACE method
-
single copy of MLCE gene, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparison and phylogenetic analysis
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
molecular biology
-
putative embryonic seCL148 product is most closely related to medaka choriolysin L
degradation
-
first choriolysin H swells the inner layer of egg envelope by limited digestion, and then choriolysin L solubilizes the swollen part of it completely