Information on EC 3.4.24.66 - choriolysin L

New: Word Map on EC 3.4.24.66
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Elopocephala

EC NUMBER
COMMENTARY hide
3.4.24.66
-
RECOMMENDED NAME
GeneOntology No.
choriolysin L
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-/-7-(4-methyl)coumarylamide
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
177529-15-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
hydrolysis at about 8% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
-
casein + H2O
?
show the reaction diagram
chorion + H2O
?
show the reaction diagram
EVLPLDNPPPA + H2O
EVLPLD + NPPPA
show the reaction diagram
-
specific activity: 8.66 nmol/min/microgram protein
-
-
?
EVQPPDSPLSI + H2O
EVQPPD + SPLSI
show the reaction diagram
-
location: D387/S388 in ChgL (ZI-1,2). Specific activity: 136 nmol/30min/microgram enzyme
-
-
?
GSKGSDETFH + H2O
GSKGS + DETFH
show the reaction diagram
-
-
-
-
?
PGKNPNTPPIG + H2O
PGKNPN + TPPIG
show the reaction diagram
-
location: N220/T221 in ChgH (ZI-1,2). Specific activity: 23.9 nmol/30min/microgram enzyme
-
-
?
PKLFQDGKPSN + H2O
PKLFQ + DGKPSN
show the reaction diagram
-
location: Q136/D137 in ChgH (ZI-1,2). Specific activity: 0.2 nmol/30min/microgram enzyme
-
-
?
PSKRPEAPGVP + H2O
PSKRPE + APGVP
show the reaction diagram
-
specific activity: 0.56 nmol/min/microgram protein
-
-
?
RPTFGRPGIT + H2O
RPTFG + RPGIT
show the reaction diagram
-
-
-
-
?
RPTIGRPGFT + H2O
RPTIG + RPGFT
show the reaction diagram
-
-
-
-
?
RPTLGEPTHT + H2O
RPTLG + EPTHT
show the reaction diagram
-
-
-
-
?
RPTLGGPKGS + H2O
RPTLG + GPKGS
show the reaction diagram
-
-
-
-
?
Suc-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
best substrate of peptidyl 4-methylcoumarin 7-amides
-
-
-
SVPVVRTSQAA + H2O
SVPVVR + TSQAA
show the reaction diagram
-
specific activity: 19.5 nmol/min/microgram protein
-
-
?
Swollen chorion + H2O
?
show the reaction diagram
VPFELRYPVPA + H2O
VPFELR + YPVPA
show the reaction diagram
-
specific activity: 10.36 nmol/min/microgram protein
-
-
?
VPFEQRYPVPA + H2O
VPFEQR + YPVPA
show the reaction diagram
-
location: R73/Y74 in ChgL (ZI-3). Specific activity: 76.3 nmol/30min/microgram enzyme
-
-
?
YPSKPQTPTET + H2O
YPSKPQ + TPTET
show the reaction diagram
-
specific activity: 2.71 nmol/min/microgram protein
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Swollen chorion + H2O
?
show the reaction diagram
-
i.e. inner layer of chorion that has been swollen by previous action of choriolysin H, EC 3.4.24.67, essential reaction in choriolysis, component of embryo-secreted hatching enzyme that digests egg envelope
-
-
-
additional information
?
-
-
egg envelope digestion: by electron microscopy it is shown that the egg envelope becomes swollen after treatment with the purified EHE. The EHE cleavage sites on the zona pellucida (ZP) protein of the egg envelope are located in the N-terminal repeat regions
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Calcium
-
0.00538 mg/mg protein, inductively coupled plasma emission analysis
Cu2+
-
partly reactivates EDTA-inactivated apoenzyme
Magnesium
-
0.00164 mg/mg protein, inductively coupled plasma emission analysis
additional information
-
no cobalt, manganese or iron detected by inductively coupled plasma emission analysis
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
48
EVQPPDSPLSI
-
pH 8.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
-
caseinolytic activity
8.6
-
for caseinolytic activity
additional information
-
pI: 9.8
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
-
about 50% of maximal caseinolytic activity at pH 6.5 and 8.9
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
in stage 25 embryos, strong expression of LCE found in branchial arches
Manually annotated by BRENDA team
-
in stage 19 embryos, LCE gene transcripts detected in a U-shaped cell mass at the anterior of the forebrain
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
at the periphery, immunocytochemical detection
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23100
-
Oryzias latipes, deduced from nucleotide sequence
additional information
-
amino acid composition
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
1 * 25500, Oryzias latipes, SDS-PAGE
monomer
-
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from hatching liquid
-
partly purified by gel filtration and cation-exchange column
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
inserted into a pGEM-T easy vector, cloning of full-length c-DNA by 5'- and 3'-RACE method
-
single copy of MLCE gene, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparison and phylogenetic analysis
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation
-
first choriolysin H swells the inner layer of egg envelope by limited digestion, and then choriolysin L solubilizes the swollen part of it completely
molecular biology
-
putative embryonic seCL148 product is most closely related to medaka choriolysin L