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Literature summary for 3.4.24.66 extracted from
- Mechanism of egg envelope digestion by hatching enzymes, HCE and LCE in medaka, Oryzias latipes (2010), J. Biochem., 148, 439-448.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 48 | - |
EVQPPDSPLSI | pH 8.0, 30°C | Oryzias latipes |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryzias latipes | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Oryzias latipes | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| EVQPPDSPLSI + H2O | location: D387/S388 in ChgL (ZI-1,2). Specific activity: 136 nmol/30min/microgram enzyme | Oryzias latipes | EVQPPD + SPLSI | - |
? | |
| additional information | cleavage sites of HCE and LCE on the egg envelope that are primarily constructed of two groups of subunit proteins, ZI-1,2 and ZI-3, are determined. LCE cleaves the middle of the zona pellucida (ZP) domain of ZI-1,2, in addition to the upstream of the trefoil domain of ZI-1,2 and the ZP domain of ZI-3. This middle site is in the intervening sequence connecting two subdomains of the ZP domain. Cleaving this site results in the solubilization of the swollen egg envelope by the disruption of the filamentous structure that is formed by the non-covalent polymerization of ZP domains | Oryzias latipes | ? | - |
? | |
| PGKNPNTPPIG + H2O | location: N220/T221 in ChgH (ZI-1,2). Specific activity: 23.9 nmol/30min/microgram enzyme | Oryzias latipes | PGKNPN + TPPIG | - |
? | |
| PKLFQDGKPSN + H2O | location: Q136/D137 in ChgH (ZI-1,2). Specific activity: 0.2 nmol/30min/microgram enzyme | Oryzias latipes | PKLFQ + DGKPSN | - |
? | |
| VPFEQRYPVPA + H2O | location: R73/Y74 in ChgL (ZI-3). Specific activity: 76.3 nmol/30min/microgram enzyme | Oryzias latipes | VPFEQR + YPVPA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LCE | - |
Oryzias latipes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Oryzias latipes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Oryzias latipes |
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Release 2023.1 (January 2023)
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