3.4.23.12: nepenthesin

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For detailed information about nepenthesin, go to the full flat file.

Word Map on EC 3.4.23.12

3.4.23.12

pitcher

carnivorous

pepsin

prey

gracilis

low-ph

hdx-ms

forbidden

asteraceae

acid-denatured

carnivory

Reaction

Similar to pepsin, but also cleaves on either side of Asp and at Lys-/-Arg =

Synonyms

EC 3.4.99.4, More, Nep I, Nep II, Nep1, nepenthacin, nepenthasin;aspartyl endopeptidase, nepenthes aspartic protease, Nepenthesin, nepenthesin I, nepenthesin II, nepenthesin IIa, nepenthesin IIb, NepI, NEPII, proteinase, nepenthes acid

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.23 Aspartic endopeptidases

3.4.23.12 nepenthesin

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Results	in table
238	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
1	Crystallization (Commentary)
5	Expression
1	General Information
36	Inhibitors
1	Localization
7	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
5	Organic Solvent Stability
28	Organism
6	pH Optimum
4	pH Range
6	pH Stability
2	pI Value
2	Posttranslational Modification
8	Purification (Commentary)
3	Reaction
1	Reaction Type
27	Reference
19	Source Tissue
1	Specific Activity [micromol/min/mg]
49	Substrates and Products (Substrate)
10	Subunits
29	Synonyms
9	Temperature Optimum [°C]
3	Temperature Range [°C]
5	Temperature Stability [°C]

Expression

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Expression on EC 3.4.23.12 - nepenthesin

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proteolytic activity in pitcher fluid is induced by the phytohormone jasmonic acid but not by salicylic acid

2 entries

the highest and most significant induction of gene expression is achieved after addition of NH4Cl, the lowest after addition of chitin

Nepenthes northiana x Mixta maxima hybrid

755054

the presence of dipteran larvae in the pitcher is correlated with lower fluid proteolytic activity although this effect is absent in pitchers that also have high concentrations of fluid microbes

Nepenthes gracilis

753939

treatment with chitin triggers the expression of nepenthesin I gene similar to jasmonic acid treatment, and induces protease activity in the fluid. Gene expression of isoform Nep I is slightly but significantly upregulated after 12 and 24 h and strongly after 48 h of fruit fly treatment compared with the control

Nepenthes alata

752493