3.4.23.12: nepenthesin

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For detailed information about nepenthesin, go to the full flat file.

Reaction

Similar to pepsin, but also cleaves on either side of Asp and at Lys-/-Arg =

Synonyms

EC 3.4.99.4, More, Nep I, Nep II, Nep1, nepenthacin, nepenthasin;aspartyl endopeptidase, nepenthes aspartic protease, Nepenthesin, nepenthesin I, nepenthesin II, nepenthesin IIa, nepenthesin IIb, NepI, NEPII, proteinase, nepenthes acid

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.23 Aspartic endopeptidases

                3.4.23.12 nepenthesin

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Results	in table
238	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
1	Crystallization (Commentary)
5	Expression
1	General Information
36	Inhibitors
1	Localization
7	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
5	Organic Solvent Stability
28	Organism
6	pH Optimum
4	pH Range
6	pH Stability
2	pI Value
2	Posttranslational Modification
8	Purification (Commentary)
3	Reaction
1	Reaction Type
27	Reference
19	Source Tissue
1	Specific Activity [micromol/min/mg]
49	Substrates and Products (Substrate)
10	Subunits
29	Synonyms
9	Temperature Optimum [°C]
3	Temperature Range [°C]
5	Temperature Stability [°C]

pH Stability

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pH Stability on EC 3.4.23.12 - nepenthesin

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

1.5 - 4

2 entries

2.9

Nepenthes alata

-

at pH 2.9 at 37°C, activity decreased very slowly on longer incubation, about 60% and 30% of the original activity are retained after 30 days and 65 days, respectively

717394

3 - 10

Nepenthes distillatoria

-

when nepenthesin I is incubated at pH 3.0 at 60°C for 30 days, about 30% of the original activity remains. When incubated at pH 3.0-10.0 at 37°C for 30 days, the enzyme retains 80% or more of activity

717394

3 - 9

Drosera peltata

-

40°C, highest stability at pH 3.0-9.0

36773

5

Drosera peltata

-

60°C, highest stability at pH 5

36773

1.5 - 4

Nepenthes gracilis

Q766C2, Q766C3

the recombinant isoform NepI retains about 45%, 80%, 99%, 85% and 55% activity after 7 days at pH 1.5, 2.0, 3.0 and 4.0, respectively

752478

1.5 - 4

Nepenthes gracilis

Q766C2, Q766C3

the recombinant isoform NepII retains about 65%, 90%, 100%, 65% and 30% activity after 7 days at pH 1.5, 2.0, 3.0 and 4.0, respectively

752478