3.4.23.12: nepenthesin

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For detailed information about nepenthesin, go to the full flat file.

Word Map on EC 3.4.23.12

3.4.23.12

pitcher

carnivorous

pepsin

prey

gracilis

low-ph

hdx-ms

forbidden

asteraceae

acid-denatured

carnivory

Reaction

Similar to pepsin, but also cleaves on either side of Asp and at Lys-/-Arg =

Synonyms

EC 3.4.99.4, More, Nep I, Nep II, Nep1, nepenthacin, nepenthasin;aspartyl endopeptidase, nepenthes aspartic protease, Nepenthesin, nepenthesin I, nepenthesin II, nepenthesin IIa, nepenthesin IIb, NepI, NEPII, proteinase, nepenthes acid

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.23 Aspartic endopeptidases

3.4.23.12 nepenthesin

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Results	in table
238	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
1	Crystallization (Commentary)
5	Expression
1	General Information
36	Inhibitors
1	Localization
7	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
5	Organic Solvent Stability
28	Organism
6	pH Optimum
4	pH Range
6	pH Stability
2	pI Value
2	Posttranslational Modification
8	Purification (Commentary)
3	Reaction
1	Reaction Type
27	Reference
19	Source Tissue
1	Specific Activity [micromol/min/mg]
49	Substrates and Products (Substrate)
10	Subunits
29	Synonyms
9	Temperature Optimum [°C]
3	Temperature Range [°C]
5	Temperature Stability [°C]

Purification

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Purification on EC 3.4.23.12 - nepenthesin

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4 entries

by gel filtration, ion exchange chromatography, and pepstatin affinity chromatography, native enzyme to homogeneity

Nepenthes distillatoria

668766

DEAE-cellulose column chromatography, pepstatin column chromatography, MonoQ column chromatography, and Sephacryl S-200 gel filtration

2 entries

nepenthesin I and II

Nepenthes distillatoria

649776