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3.4.23.12: nepenthesin

This is an abbreviated version!
For detailed information about nepenthesin, go to the full flat file.

Word Map on EC 3.4.23.12

Reaction

Similar to pepsin, but also cleaves on either side of Asp and at Lys-/-Arg =

Synonyms

EC 3.4.99.4, More, Nep I, Nep II, Nep1, nepenthacin, nepenthasin;aspartyl endopeptidase, nepenthes aspartic protease, Nepenthesin, nepenthesin I, nepenthesin II, nepenthesin IIa, nepenthesin IIb, NepI, NEPII, proteinase, nepenthes acid

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.12 nepenthesin

Inhibitors

Inhibitors on EC 3.4.23.12 - nepenthesin

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-(2-aminoethyl)benzenesulfonyl fluoride
about 70% residual activity at 0.1 mM
CoSO4
CuCl2
cupric ions
-
strong inhibition of nepenthesin I and II by diazoacetyl-DL-norleucine in presence of cupric ions, no inactivation in absence of cupric ions
diazoacetyl-DL-norleucine
-
strong inhibition of nepenthesin I and II in presence of cupric ions, no inactivation in absence of cupric ions
Diazoacetyl-DL-norleucine methyl ester
-
i.e. DAN
dithiothreitol
-
isoforms nethepsin I, IIa and IIb are completely inactivated in the presence of 40-70 mM dithiothreitol
DL-Norleucine methyl ester
DMSO
about 65% residual activity at 0.1 mM
E-64
about 97% residual activity at 0.02 mM
guanidine hydrochloride
isoform NepII shows about 85% residual activity at 4 M, about 50% residual activity at 5 M, and is completely inhibited at 6 M guanidine hydrochloride; isoform NepI shows about 50% residual activity at 1 M and is completely inhibited at 4 M guanidine hydrochloride
HgCl2
N-(diazoacetyl)-N-(2,4-dinitrophenyl)ethylenediamine
-
-
pepstatin
pepstatin A
Phenanthroline
about 65% residual activity at 0.1 mM
Urea
isoform NepI shows about 85% residual activity at 4 M and is completely inhibited at 6 M urea
additional information
-