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3.4.22.6: chymopapain

This is an abbreviated version!
For detailed information about chymopapain, go to the full flat file.

Word Map on EC 3.4.22.6

Reaction

Similar to that of papain =

Synonyms

Chymopapain, chymopapain A, chymopapain B, chymopapain isoform II, chymopapain isoform III, chymopapain isoform IV, Chymopapain S, CpXCP8, EC 3.4.4.11, More, papaya proteinase II, PPII

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.6 chymopapain

Purification

Purification on EC 3.4.22.6 - chymopapain

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
addition of polyethyleneglycol facilitates purification
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chymopapain B
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chymopapain S
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commercial preparation
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enzyme is semi-purified with from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration
native enzyme from latex
supports: phenyl-Hg+ CH3COO- and glutathione-S-S-(2-pyridyl), further purification through the S-thiol poly(ethylene glycol) derivatization technique
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