3.4.21.103: physarolisin

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For detailed information about physarolisin, go to the full flat file.

Reaction

Milk clotting activity. Preferential cleavage of Gly8-/-Ser in B chain of insulin most rapidly, followed by Leu11!Val, Cys(SO3H)19-/-Gly and Phe24-/-Phe. No action on Ac-Phe-Tyr(I)2. =

Synonyms

EC 3.4.23.27, EC 3.4.23.6, EC 3.4.4.17, physarolisin, physarolisin I, physarolysin II, Physarum aspartic proteinase, proteinase, Dictyostelium discoideum aspartic, proteinase, Dictyostelium discoideum aspartic, E, proteinase, Physarum flavicomum aspartic, proteinase, Physarum polycephalum acid

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.103 physarolisin

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Results	in table
1	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
2	General Stability
15	Inhibitors
3	Localization
10	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
8	Organism
6	pH Optimum
2	pH Range
7	pH Stability
3	Posttranslational Modification
5	Purification (Commentary)
1	Reaction
1	Reaction Type
8	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
28	Substrates and Products (Substrate)
4	Subunits
11	Synonyms
3	Temperature Optimum [°C]
2	Temperature Range [°C]
8	Temperature Stability [°C]

General Stability

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General Stability on EC 3.4.21.103 - physarolisin

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

the active enzyme is markedly unstable due to rapid autolysis, t1/2: 5 min at 18°C, pH 4.2

Physarum polycephalum

-

651328

thiol reagent required for stability

Physarum flavicomum

-

647479