3.4.21.103: physarolisin

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For detailed information about physarolisin, go to the full flat file.

Reaction

Milk clotting activity. Preferential cleavage of Gly8-/-Ser in B chain of insulin most rapidly, followed by Leu11!Val, Cys(SO3H)19-/-Gly and Phe24-/-Phe. No action on Ac-Phe-Tyr(I)2. =

Synonyms

EC 3.4.23.27, EC 3.4.23.6, EC 3.4.4.17, physarolisin, physarolisin I, physarolysin II, Physarum aspartic proteinase, proteinase, Dictyostelium discoideum aspartic, proteinase, Dictyostelium discoideum aspartic, E, proteinase, Physarum flavicomum aspartic, proteinase, Physarum polycephalum acid

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.103 physarolisin

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Results	in table
1	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
2	General Stability
15	Inhibitors
3	Localization
10	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
8	Organism
6	pH Optimum
2	pH Range
7	pH Stability
3	Posttranslational Modification
5	Purification (Commentary)
1	Reaction
1	Reaction Type
8	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
28	Substrates and Products (Substrate)
4	Subunits
11	Synonyms
3	Temperature Optimum [°C]
2	Temperature Range [°C]
8	Temperature Stability [°C]

pH Stability

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pH Stability on EC 3.4.21.103 - physarolisin

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2 - 3

Physarum flavicomum

-

maximal stability

647479

2 - 9

Dictyostelium discoideum

-

25°C, 24 h, stable

647478

3 - 6

2 entries

7

Physarum flavicomum

-

unstable

647479

8

Physarum polycephalum

-

37°C, 15 min, 30% loss of activity

647477

9

Physarum polycephalum

-

37°C, 15 min, 45% loss of activity

647477

3 - 6

Dictyostelium discoideum

-

25°C, 48 h, stable

647478

3 - 6

Physarum polycephalum

-

-

647477