3.4.21.103: physarolisin
This is an abbreviated version!
For detailed information about physarolisin, go to the full flat file.
Word Map on EC 3.4.21.103
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3.4.21.103
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3.4.23.6
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proteinases
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r-factors
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endothiapepsin
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renin
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rhizopuspepsin
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rhizopus
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root-mean-square
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scissile
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beta-hairpins
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pepstatin
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non-hydrogen
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penicillopepsin
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serine-carboxyl
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statine-containing
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hydroxyethylene
- 3.4.21.103
-
3.4.23.6
- proteinases
-
r-factors
- endothiapepsin
- renin
- rhizopuspepsin
- rhizopus
-
root-mean-square
-
scissile
-
beta-hairpins
- pepstatin
-
non-hydrogen
- penicillopepsin
-
serine-carboxyl
-
statine-containing
-
hydroxyethylene
Reaction
Milk clotting activity. Preferential cleavage of Gly8-/-Ser in B chain of insulin most rapidly, followed by Leu11!Val, Cys(SO3H)19-/-Gly and Phe24-/-Phe. No action on Ac-Phe-Tyr(I)2. =
Synonyms
EC 3.4.23.27, EC 3.4.23.6, EC 3.4.4.17, physarolisin, physarolisin I, physarolysin II, Physarum aspartic proteinase, proteinase, Dictyostelium discoideum aspartic, proteinase, Dictyostelium discoideum aspartic, E, proteinase, Physarum flavicomum aspartic, proteinase, Physarum polycephalum acid
ECTree
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Reference
Reference on EC 3.4.21.103 - physarolisin
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Murakami-Murofushi, K.; Takahashi, T.; Minowa, Y.; Lino, S.; Takeuchi, T.; Kitagaki-Ogawa, H.; Murofushi, H.; Takahshi, K.
Purification and characterization of a novel intracellular acid proteinase from the plasmodia of a true slime mold, Physarum polycephalum
J. Biol. Chem.
32
19898-19903
1990
Physarum polycephalum
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North, M.J.; Whyte, A.
Purification and characterization of two acid proteinases from Dictyostelium discoideum
J. Gen. Microbiol.
130
123-134
1984
Dictyostelium discoideum
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Henney, H.R.; White, H.U.
Characterization of a lysosomal proteinase purified from haploid cells of Physarum flavicomum undergoing encystment
Can. J. Bot.
61
1357-1366
1983
Physarum flavicomum
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Murakami-Murofushi, K.; Takahashi, T.; Murofushi, H.; Takahashi, K.
A novel intracellular acid proteinase from the plasmodia of a true slime mold, Physarum polycephalum
Adv. Exp. Med. Biol.
362
565-568
1995
Physarum polycephalum
Nishii, W.; Ueki, T.; Miyashita, R.; Kojima, M.; Kim, Y.T.; Sasaki, N.; Murakami-Murofushi, K.; Takahashi, K.
Structural and enzymatic characterization of physarolisin (formerly physaropepsin) proves that it is a unique serine-carboxyl proteinase
Biochem. Biophys. Res. Commun.
301
1023-1029
2003
Physarum polycephalum
Nishii, W.; Kuriyama, H.; Takahashi, K.
The Physarum polycephalum php gene encodes a unique cold-adapted serine-carboxyl peptidase, physarolisin II
FEBS Lett.
546
340-344
2003
Physarum polycephalum
Murakami-Murofushi, K.; Nishii, W.; Takahashi, K.
Physarolisin
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1891-1893
2004
Physarum polycephalum (Q8MZS4)
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