3.4.21.103: physarolisin

This is an abbreviated version!
For detailed information about physarolisin, go to the full flat file.

Word Map on EC 3.4.21.103

3.4.21.103

3.4.23.6

proteinases

r-factors

endothiapepsin

renin

rhizopuspepsin

rhizopus

root-mean-square

scissile

beta-hairpins

pepstatin

non-hydrogen

penicillopepsin

serine-carboxyl

statine-containing

hydroxyethylene

Reaction

Milk clotting activity. Preferential cleavage of Gly8-/-Ser in B chain of insulin most rapidly, followed by Leu11!Val, Cys(SO3H)19-/-Gly and Phe24-/-Phe. No action on Ac-Phe-Tyr(I)2. =

Synonyms

EC 3.4.23.27, EC 3.4.23.6, EC 3.4.4.17, physarolisin, physarolisin I, physarolysin II, Physarum aspartic proteinase, proteinase, Dictyostelium discoideum aspartic, proteinase, Dictyostelium discoideum aspartic, E, proteinase, Physarum flavicomum aspartic, proteinase, Physarum polycephalum acid

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.21 Serine endopeptidases

3.4.21.103 physarolisin

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Results	in table
1	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
2	General Stability
15	Inhibitors
3	Localization
10	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
8	Organism
6	pH Optimum
2	pH Range
7	pH Stability
3	Posttranslational Modification
5	Purification (Commentary)
1	Reaction
1	Reaction Type
8	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
28	Substrates and Products (Substrate)
4	Subunits
11	Synonyms
3	Temperature Optimum [°C]
2	Temperature Range [°C]
8	Temperature Stability [°C]

Inhibitors

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Inhibitors on EC 3.4.21.103 - physarolisin

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DAN

Physarum polycephalum

Q8MZS4

in the presence of cupric ions

665089

DFP

2 entries

Diazoacetyl-D,L-norleucine methyl ester

Physarum polycephalum

in presence of Cu2+

647477

Diazoacetyl-DL-norleucine methyl ester

2 entries

diisopropyl fluorophosphate

Physarum polycephalum

23% inhibition by 0.5 mM, 76% inhibition by 10 mM

651328

pepstatin

3 entries

PMSF

Physarum polycephalum

10 mM, 21% inhibition

649268

SH-reagents

Physarum flavicomum

647479

tosyl-L-Leu-chloromethylketone

Physarum polycephalum

10 mM, 18% inhibition

649268

additional information

2 entries