3.4.11.10: bacterial leucyl aminopeptidase
This is an abbreviated version!
For detailed information about bacterial leucyl aminopeptidase, go to the full flat file.
Word Map on EC 3.4.11.10
Reaction
release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids =
Synonyms
AAP, Aeromonas proteolytica aminopeptidase, Aminopeptidase, aminopeptidase A, aminopeptidase A (bacteria), aminopeptidase Ap1, aminopeptidase II, AP-II, API, APII, AVP, bacterial leucine aminopeptidase, bacterial M17 aminopeptidase, BSAP, Bsu aminopeptidase, BsuAP, CGase, cysteinylglycinase, double-zinc aminopeptidase, extracellular aminopeptidase, FgLAP, HpM17AP, LAP, LAPII, leucine aminopeptidase, leucine aminopeptidase II, leucine APN, Leucyl aminopeptidase, M17 aminopeptidase, M17 metallo-aminopeptidase, More, MtLAP, PepA, Peptidase A, pepZ, PhpA, ribosomal-bound aminopeptidase, rLAP55, Rv2213, SSAP, TAP, TH-2, thermophilic aminopeptidase, thermostable leucine aminopeptidase, Vibrio aminopeptidase, VpAP, ywaD
ECTree
3.4.11.10 bacterial leucyl aminopeptidaseAdvanced search results
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Turnover Number on EC 3.4.11.10 - bacterial leucyl aminopeptidase
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
192000
L-Ala-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1mM MnCl2, at 37°C
59900
L-Arg-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1mM MnCl2, at 37°C
288100
L-Cys-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1mM MnCl2, at 37°C
33800
L-Phe-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1mM MnCl2, at 37°C
16200
L-Thr-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1mM MnCl2, at 37°C
0.000853
recombinant human methionine-interferon alpha-2b
pH 6.0, 37°C
-
82
L-Arg-p-nitroanilide
-
mutant enzyme D198P/F221E, at pH 8.0 and 37°C
46.7
L-cystinyl-p-nitroanilide
-
mutant enzyme D198I/F221W, at pH 8.0 and 37°C
0.017
L-Leu-4-nitroanilide
apparent value, mutant enzyme D99M, in 10 mM Tricine buffer, pH 8.0, at 25°C
0.035
L-Leu-4-nitroanilide
-
mutant enzyme D380E, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
0.048
L-Leu-4-nitroanilide
-
mutant enzyme D380V, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
0.113
L-Leu-4-nitroanilide
-
mutant enzyme Y352L, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
0.135
L-Leu-4-nitroanilide
-
mutant enzyme D380N, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
0.153
L-Leu-4-nitroanilide
-
mutant enzyme Y352D, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
0.29
L-Leu-4-nitroanilide
60°C, recombinant wild-type enzyme
0.303
L-Leu-4-nitroanilide
-
wild type enzyme, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
0.46
L-Leu-4-nitroanilide
60°C, recombinant mutant H191L
0.7
L-Leu-4-nitroanilide
mutant enzyme M180A, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
0.817
L-Leu-4-nitroanilide
pH 8.0, 37°C, recombinant His6-tagged enzyme, without preincubation
0.86
L-Leu-4-nitroanilide
60°C, recombinant mutant H227L
6.31
L-Leu-4-nitroanilide
-
pH 8.0, 55°C, recombinant wild-type enzyme
7
L-Leu-4-nitroanilide
mutant enzyme S228A, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
15.8
L-Leu-4-nitroanilide
pH 8.0, 37°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C)
48.45
L-Leu-4-nitroanilide
pH 8.0, 70°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C)
60
L-Leu-4-nitroanilide
mutant enzyme D118N, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
65
L-Leu-4-nitroanilide
wild type enzyme, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
246000
L-Leu-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1mM MnCl2, at 37°C
128
L-Leu-p-nitroanilide
-
mutant enzyme D198F/F221I, at pH 8.0 and 37°C
3
L-leucine 4-nitroanilide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
5.3
L-leucine 4-nitroanilide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
42
L-leucine 4-nitroanilide
-
1-ethyl-3(3-dimethylaminopropyl)-carbodiimide treated enzyme
3.8
L-leucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
9.3
L-leucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
283200
L-Met-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1mM MnCl2, at 37°C
7.5
thionoleucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
12.2
thionoleucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
7.7
thionoleucine-S-anilide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
16.9
thionoleucine-S-anilide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
23.3
thionoleucine-S-anilide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
additional information
additional information
-
penultimative substituents influence rate of hydrolysis
-
additional information
additional information
-
penultimative substituents influence rate of hydrolysis
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