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0.14
Gly-7-amido-4-methylcoumarin
-
pH 8.0, 37°C
10
L-Ala-4-nitroanilide
pH 8.0, 30°C
0.231
L-Ala-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
0.26
L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin
-
pH 8.0, 37°C
0.6
L-Arg-4-nitroanilide
pH 8.0, 30°C
0.078
L-Arg-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
1 - 7.51
L-Arg-p-nitroanilide
0.077
L-Cys-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
0.056 - 0.11
L-cystinyl-p-nitroanilide
0.01 - 4.4
L-Leu-4-nitroanilide
0.038 - 0.18
L-Leu-7-amido-4-methylcoumarin
25
L-Leu-Gly-Gly
-
pH 7.6-7.8, 60°C
0.57 - 1.79
L-Leu-p-nitroanilide
0.01 - 15
L-leucine 4-nitroanilide
45 - 78
L-leucine anilide
0.7
L-leucine ethyl ester
-
-
32 - 50
L-leucine-4-anisidide
0.22 - 0.85
L-Lys-4-nitroanilide
0.093 - 0.24
L-Met-7-amido-4-methylcoumarin
0.066
L-Phe-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
0.06
L-Thr-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
19
L-Val-4-nitroanilide
pH 8.0, 30°C
0.97
Leu-4-nitroanilide
pH 8.5, 50°C, recombinant enzyme
0.1 - 0.43
Leu-beta-naphthylamide
0.97
leucine-4-nitroaniline
-
pH 8.5, 60°C
0.96
Met-beta-naphthylamide
-
-
0.52
Phe-beta-naphthylamide
-
-
0.02726
recombinant human methionine-interferon alpha-2b
pH 6.0, 37°C
-
4 - 7
thionoleucine-4-anisidide
4 - 11
thionoleucine-S-anilide
0.19
Val-beta-naphthylamide
-
-
additional information
additional information
-
0.39
Cys-Gly
-
recombinant enzyme, at pH 8.0
0.44
Cys-Gly
-
recombinant enzyme, at pH 7.3
0.48
Cys-Gly
-
native enzyme, at pH 7.3
1
L-Arg-p-nitroanilide
-
wild type enzyme, at pH 8.0 and 37°C
7.51
L-Arg-p-nitroanilide
-
mutant enzyme D198P/F221E, at pH 8.0 and 37°C
0.056
L-cystinyl-p-nitroanilide
-
mutant enzyme D198I/F221W, at pH 8.0 and 37°C
0.11
L-cystinyl-p-nitroanilide
-
wild type enzyme, at pH 8.0 and 37°C
0.01
L-Leu-4-nitroanilide
mutant enzyme D118N, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
0.0106
L-Leu-4-nitroanilide
pH 8.0, 25°C, mature wild-type enzyme
0.013
L-Leu-4-nitroanilide
wild type enzyme, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
0.025
L-Leu-4-nitroanilide
mutant enzyme S228A, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
0.0317
L-Leu-4-nitroanilide
apparent value, mutant enzyme D99M, in 10 mM Tricine buffer, pH 8.0, at 25°C
0.245
L-Leu-4-nitroanilide
pH 8.0, 37°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C)
0.25
L-Leu-4-nitroanilide
-
pH 8.0, 37°C, APII
0.311
L-Leu-4-nitroanilide
pH 8.0, 70°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C)
0.34
L-Leu-4-nitroanilide
mutant enzyme M180A, in 50 mM Tricine buffer, 1.0 mM ZnSO4, and 200 mM KCl, at pH 8.0 and 25°C
0.658
L-Leu-4-nitroanilide
-
pH 7.6-7.8, 60°C
0.9
L-Leu-4-nitroanilide
60°C, recombinant wild-type enzyme
0.902
L-Leu-4-nitroanilide
pH 8.0, 37°C, recombinant His6-tagged enzyme, without preincubation
0.95
L-Leu-4-nitroanilide
-
wild type enzyme, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
1
L-Leu-4-nitroanilide
-
pH 8.5, 45°C, API
1.2
L-Leu-4-nitroanilide
60°C, recombinant mutant H191L
2.1
L-Leu-4-nitroanilide
-
pH 8.0, 55°C, recombinant wild-type enzyme
2.12
L-Leu-4-nitroanilide
-
mutant enzyme Y352D, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
2.83
L-Leu-4-nitroanilide
-
mutant enzyme Y352L, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
3.05
L-Leu-4-nitroanilide
-
mutant enzyme D380N, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
3.39
L-Leu-4-nitroanilide
-
mutant enzyme D380E, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
3.41
L-Leu-4-nitroanilide
-
mutant enzyme D380V, in 25 mM Tris-HCl buffer, pH 8.0, at 60°C
3.6
L-Leu-4-nitroanilide
pH 8.0, 30°C
4.4
L-Leu-4-nitroanilide
60°C, recombinant mutant H227L
0.038
L-Leu-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
0.18
L-Leu-7-amido-4-methylcoumarin
-
pH 8.0, 37°C
0.57
L-Leu-p-nitroanilide
-
wild type enzyme, at pH 8.0 and 37°C
1.15
L-Leu-p-nitroanilide
-
mutant enzyme D198F/F221I, at pH 8.0 and 37°C
1.72
L-Leu-p-nitroanilide
-
recombinant enzyme, at pH 7.3
1.79
L-Leu-p-nitroanilide
-
recombinant enzyme, at pH 8.0
0.01
L-leucine 4-nitroanilide
pH 8.0, 25°C
0.019
L-leucine 4-nitroanilide
-
-
0.019 - 0.0215
L-leucine 4-nitroanilide
-
native enzyme, buffer dependent
0.02
L-leucine 4-nitroanilide
-
-
0.021
L-leucine 4-nitroanilide
-
native enzyme
0.028
L-leucine 4-nitroanilide
-
-
0.032
L-leucine 4-nitroanilide
-
diethylpyrocarbonate-treated enzyme
0.055
L-leucine 4-nitroanilide
-
1-ethyl-3(3-dimethylaminopropyl)-carbodiimide treated enzyme
0.06
L-leucine 4-nitroanilide
-
nitro-enzyme
0.126
L-leucine 4-nitroanilide
-
photoinactivated enzyme
0.136
L-leucine 4-nitroanilide
-
azo-enzyme
4.6
L-leucine 4-nitroanilide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
7.8
L-leucine 4-nitroanilide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
15
L-leucine 4-nitroanilide
-
pH 8.0, 25°C, enzyme with 2 Zn2+ bound
45
L-leucine anilide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
61
L-leucine anilide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
78
L-leucine anilide
-
pH 8.0, 25°C, enzyme with 2 Zn2+ bound
32
L-leucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
34
L-leucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
50
L-leucine-4-anisidide
-
pH 8.0, 25°C, enzyme with 2 Zn2+ bound
0.22
L-Lys-4-nitroanilide
-
pH 8.0, 37°C
0.85
L-Lys-4-nitroanilide
pH 8.0, 30°C
0.093
L-Met-7-amido-4-methylcoumarin
-
recombinant enzyme, in 0.1 M glycine buffer, pH 8.5 containing 1 mM MnCl2, at 37°C
0.24
L-Met-7-amido-4-methylcoumarin
-
pH 8.0, 37°C
0.1
Leu-beta-naphthylamide
-
-
0.43
Leu-beta-naphthylamide
-
-
0.05
Leu-Phe
-
-
4
thionoleucine-4-anisidide
-
pH 8.0, 25°C, enzyme with 2 Cd2+ bound
4
thionoleucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
5
thionoleucine-4-anisidide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
7
thionoleucine-4-anisidide
-
pH 8.0, 25°C, enzyme with 2 Zn2+ bound
4
thionoleucine-S-anilide
-
pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound
8
thionoleucine-S-anilide
-
pH 8.0, 25°C, enzyme with 2 Cd2+ bound
10
thionoleucine-S-anilide
-
pH 8.0, 25°C, enzyme with 2 Zn2+ bound
11
thionoleucine-S-anilide
-
pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound
additional information
additional information
kinetics
-
additional information
additional information
-
amides and dipeptides
-
additional information
additional information
-
amides and dipeptides
-
additional information
additional information
-
kinetics and thermodynamics
-
additional information
additional information
kinetics with metal-bound enzyme variants
-
additional information
additional information
-
kinetics of apoenzyme and metal-bound wild-type and mutant enzymes, isothermal titration measurement, thermodynamics, overview
-
additional information
additional information
kinetics of different native and recombinant enzyme forms, overview
-
additional information
additional information
-
kinetics of raw starch-binding by the chimeric mutant enzyme
-
additional information
additional information
-
kinetics of wild-type and methionine mutant enzymes, overview
-
additional information
additional information
-
kinetics with Zn and/or Cd-containing enzyme, overview
-
additional information
additional information
pH-dependence of kinetics, isothermal titration measurement, thermodynamics, overview
-
additional information
additional information
thermodynamics and Michaelis-Menten kinetics, overview
-