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Literature summary for 3.4.11.10 extracted from

  • Arima, J.; Uesugi, Y.; Iwabuchi, M.; Hatanaka, T.
    Change in substrate preference of Streptomyces aminopeptidase through modification of the environment around the substrate binding site (2006), Appl. Environ. Microbiol., 72, 7962-7967.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces septatus

Protein Variants

Protein Variants Comment Organism
D198A/F221T decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
D198F/F221D decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Arg-p-nitroanilide Streptomyces septatus
D198H/F221N decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
D198I/F221W decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-cystinyl-p-nitroanilide Streptomyces septatus
D198L/F221E decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Arg-p-nitroanilide Streptomyces septatus
D198L/F221M decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
D198L/F221T decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
D198L/F221W decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-cystinyl-p-nitroanilide Streptomyces septatus
D198M/F221A decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
D198M/F221G decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
D198M/F221V decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
D198M/F221W highest activity towards L-Leu-p-nitroanilide like the wild type enzyme Streptomyces septatus
D198P/F221E decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Arg-p-nitroanilide Streptomyces septatus
D198S/F221E decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Arg-p-nitroanilide Streptomyces septatus
D198S/F221N decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
D198V/F221D decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Lys-p-nitroanilide Streptomyces septatus
D198V/F221N decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Phe-p-nitroanilide Streptomyces septatus
D198W/F221D decreased activity towards L-Leu-p-nitroanilide, highest activity towards L-Arg-p-nitroanilide Streptomyces septatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
 L-cystinyl-p-nitroanilide mutant enzyme D198I/F221W, at pH 8.0 and 37°C Streptomyces septatus
0.11
-
 L-cystinyl-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
0.57
-
 L-Leu-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
1
-
 L-Arg-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
1.15
-
 L-Leu-p-nitroanilide mutant enzyme D198F/F221I, at pH 8.0 and 37°C Streptomyces septatus
7.51
-
 L-Arg-p-nitroanilide mutant enzyme D198P/F221E, at pH 8.0 and 37°C Streptomyces septatus

Organism

Organism UniProt Comment Textmining
Streptomyces septatus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
 mutant enzyme D198M/F221W, using L-Leu-p-nitroanilide as a substrate Streptomyces septatus
68
-
 wild type enzyme, using L-Leu-p-nitroanilide as a substrate Streptomyces septatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-p-nitroanilide + H2O 1% activity compared to L-Leu-p-nitroanilide Streptomyces septatus L-Ala + p-nitroaniline
-
 ?
L-Arg-p-nitroanilide + H2O 3% activity compared to L-Leu-p-nitroanilide Streptomyces septatus L-Arg + p-nitroaniline
-
 ?
L-cystinyl-p-nitroanilide + H2O 10% activity compared to L-Leu-p-nitroanilide Streptomyces septatus L-cystine + p-nitroaniline
-
 ?
L-Leu-p-nitroanilide + H2O 100% activity Streptomyces septatus L-Leu + p-nitroaniline
-
 ?
L-Lys-p-nitroanilide + H2O 24% activity compared to L-Leu-p-nitroanilide Streptomyces septatus L-Lys + p-nitroaniline
-
 ?
L-Phe-p-nitroanilide + H2O 23% activity compared to L-Leu-p-nitroanilide Streptomyces septatus L-Phe + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
SSAP
-
 Streptomyces septatus
TH-2
-
 Streptomyces septatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.26
-
 L-Arg-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
3.71
-
 L-cystinyl-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
41.3
-
 L-Leu-p-nitroanilide wild type enzyme, at pH 8.0 and 37°C Streptomyces septatus
46.7
-
 L-cystinyl-p-nitroanilide mutant enzyme D198I/F221W, at pH 8.0 and 37°C Streptomyces septatus
82
-
 L-Arg-p-nitroanilide mutant enzyme D198P/F221E, at pH 8.0 and 37°C Streptomyces septatus
128
-
 L-Leu-p-nitroanilide mutant enzyme D198F/F221I, at pH 8.0 and 37°C Streptomyces septatus