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3.2.1.97: endo-alpha-N-acetylgalactosaminidase

This is an abbreviated version!
For detailed information about endo-alpha-N-acetylgalactosaminidase, go to the full flat file.

Word Map on EC 3.2.1.97

Reaction

beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-[glycoprotein]-L-serine/L-threonine
+
H2O
=
beta-D-galactosyl-(1->3)-N-acetyl-D-galactosamine
 +
[glycoprotein]-L-serine/L-threonine

Synonyms

acetylgalactosaminidase, endo-alpha, D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetylgalactosaminohydrolase, EC 3.2.1.110, endo GalNAc-ase S, endo-alpha-acetylgalactosaminidase, endo-alpha-GalNAc-ase, endo-alpha-N-acetylgalactosaminidase, endo-alpha-N-acetylgalactosaminidase S, endo-beta-N-acetylgalactosaminidase, endo-EF, endo-GalNAc-ase S, endoEF, EngBF, EngCP, More, NagBb, SpGH101

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.97 endo-alpha-N-acetylgalactosaminidase

Engineering

Engineering on EC 3.2.1.97 - endo-alpha-N-acetylgalactosaminidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D1058N
activity in cell-free extracts of Escherichia coli almost comparable to wild-type
D1106N
D1248N
10-30% activity compared to wild-type
D1295A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
D601N
D605N
D612N
D655N
D665N
D682A
D682N
D700N
D762A
D762N
D789A
D789N
D839N
D864N
D898N
E1276Q
E1302Q
E1350Q
E679Q
E723Q
E822A
E822Q
significant decrease in activity (below 2%)
E828Q
E882Q
Escherichia coli cells expressing the mutant enzyme exhibit less than 2% of the wild-type endo-alpha-GalNAc activity
K1199A
-
site-directed mutagenesis, the mutant shows increased activity compared to the truncated wild-type enzyme
N720A
-
site-directed mutagenesis, the mutant shows increased activity compared to the truncated wild-type enzyme
Q894A
-
site-directed mutagenesis, the mutant shows increased activity compared to the truncated wild-type enzyme
S1248N
Escherichia coli cells expressing the mutant enzyme exhibit 10-30% of the wild-type endo-alpha-GalNAc activity
W748A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
W748F
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
W748Y
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
W750A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
W750F
-
site-directed mutagenesis, the mutant shows reduced activity compared to the truncated wild-type enzyme
W750Y
-
site-directed mutagenesis, the mutant shows reduced activity compared to the truncated wild-type enzyme
Y787F
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
D601N
D612N
-
activity in cell-free extracts of Escherichia coli almost comparable to wild-type
-
D762N
D658A
site-directed mutagenesis, the substrate specificity and activity is altered compared to the wild-type enzyme
D764A
site-directed mutagenesis, the substrate specificity and activity is altered compared to the wild-type enzyme
D764F
site-directed mutagenesis, the substrate specificity and activity is altered compared to the wild-type enzyme
E796A
site-directed mutagenesis, the substrate specificity and activity is altered compared to the wild-type enzyme
E796Q
site-directed mutagenesis, the substrate specificity and activity is altered compared to the wild-type enzyme
additional information