Information on EC 3.2.1.97 - endo-alpha-N-acetylgalactosaminidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.2.1.97
-
RECOMMENDED NAME
GeneOntology No.
endo-alpha-N-acetylgalactosaminidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O = 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
glycopeptide-D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetyl-galactosaminohydrolase
The enzyme catalyses the liberation of Gal-(1->3)-beta-GalNAc alpha-linked to serine or threonine residues of mucin-type glycoproteins. EngBF from the bacterium Bifidobacterium longum specifically acts on core 1-type O-glycan to release the disaccharide Gal-(1->3)-beta-GalNAc. The enzymes from the bacteria Clostridium perfringens, Enterococcus faecalis, Propionibacterium acnes and Alcaligenes faecalis show broader specificity (e.g. they can also release the core 2 trisaccharide Gal-(1->3)-beta-(GlcNAc-(1->6)-beta)-GalNAc or the core 3 disaccharide GlcNAc-(1->3)-beta-GalNAc) [1,2]. The enzyme may play an important role in the degradation and utilization of mucins having core 1 O-glycan.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acetylgalactosaminidase, endo-alpha
-
-
-
-
D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetylgalactosaminohydrolase
-
-
-
-
endo GalNAc-ase S
-
-
endo-alpha-acetylgalactosaminidase
-
-
-
-
endo-alpha-GalNAc-ase
-
-
endo-alpha-GalNAc-ase
Bacillus sp. A198
-
-
-
endo-alpha-GalNAc-ase
-
-
endo-alpha-GalNAc-ase
Bifidobacterium bifidum ATCC29521, Bifidobacterium bifidum JCM 7004
-
-
-
endo-alpha-GalNAc-ase
-
-
endo-alpha-GalNAc-ase
Bifidobacterium breve JCM 1192
-
-
-
endo-alpha-GalNAc-ase
-
-
endo-alpha-GalNAc-ase
Q3T552
-
endo-alpha-GalNAc-ase
Bifidobacterium longum JCM 1217
Q3T552
-
-
endo-alpha-GalNAc-ase
Bifidobacterium longum JCM 7054
-
-
-
endo-alpha-GalNAc-ase
Q8XMJ5
-
endo-alpha-GalNAc-ase
Clostridium perfringens 13
Q8XMJ5
-
-
endo-alpha-N-acetylgalactosaminidase
-
-
-
-
endo-alpha-N-acetylgalactosaminidase
-
-
endo-alpha-N-acetylgalactosaminidase
Bifidobacterium bifidum ATCC29521, Bifidobacterium bifidum JCM 7004
-
-
-
endo-alpha-N-acetylgalactosaminidase
-
-
endo-alpha-N-acetylgalactosaminidase
Bifidobacterium breve JCM 1192
-
-
-
endo-alpha-N-acetylgalactosaminidase
-
-
endo-alpha-N-acetylgalactosaminidase
Q3T552
-
endo-alpha-N-acetylgalactosaminidase
Bifidobacterium longum JCM 1217
Q3T552
;
-
endo-alpha-N-acetylgalactosaminidase
Bifidobacterium longum JCM 7054, Bifidobacterium longum JCM1217
-
-
-
endo-alpha-N-acetylgalactosaminidase
Q8XMJ5
-
endo-alpha-N-acetylgalactosaminidase
Clostridium perfringens 13
Q8XMJ5
-
-
endo-alpha-N-acetylgalactosaminidase
Q833X5
-
endo-alpha-N-acetylgalactosaminidase
Enterococcus faecalis NBRC3971
Q833X5
-
-
endo-alpha-N-acetylgalactosaminidase
-
-
endo-alpha-N-acetylgalactosaminidase S
-
-
endo-alpha-N-acetylgalactosaminidase S
Streptomyces sp. OH-11242
-
;
-
endo-beta-N-acetylgalactosaminidase
-
-
endo-EF
Q833X5
-
endo-EF
Enterococcus faecalis NBRC3971
Q833X5
-
-
endo-GalNAc-ase S
-
-
endo-GalNAc-ase S
Streptomyces sp. OH-11242
-
-
-
EngBF
Bifidobacterium longum JCM 1217
Q3T552
-
-
EngBF
Bifidobacterium longum JCM1217
-
-
-
EngCP
Clostridium perfringens 13
Q8XMJ5
-
-
additional information
-
the enzyme belongs to the glycoside hydrolase family 101, GH101
additional information
-
the enzyme belongs to the glycoside hydrolase family 101, GH101
additional information
-
the enzyme belongs to the glycoside hydrolase family 101, GH101
CAS REGISTRY NUMBER
COMMENTARY
59793-96-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain F-1906
-
-
Manually annotated by BRENDA team
Alcaligenes sp. F-1906
strain F-1906
-
-
Manually annotated by BRENDA team
strain A198
-
-
Manually annotated by BRENDA team
Bacillus sp. A198
strain A198
-
-
Manually annotated by BRENDA team
ATCC29521; JCM 1254; JCM 7004
-
-
Manually annotated by BRENDA team
Bifidobacterium bifidum ATCC29521
ATCC29521
-
-
Manually annotated by BRENDA team
Bifidobacterium bifidum JCM 7004
JCM 7004
-
-
Manually annotated by BRENDA team
Bifidobacterium breve JCM 1192
JCM 1192
-
-
Manually annotated by BRENDA team
JCM 1217
SwissProt
Manually annotated by BRENDA team
JCM 1217; strain JCM 1217
SwissProt
Manually annotated by BRENDA team
strain JCM1217, ATCC29521
-
-
Manually annotated by BRENDA team
Bifidobacterium longum JCM 1217
JCM 1217
SwissProt
Manually annotated by BRENDA team
Bifidobacterium longum JCM 7054
JCM 7054
-
-
Manually annotated by BRENDA team
Bifidobacterium longum JCM1217
strain JCM1217, ATCC29521
-
-
Manually annotated by BRENDA team
Brevibacillus laterosporus SS-16
strain SS-16
-
-
Manually annotated by BRENDA team
Clostridium perfringens 13
strain 13
UniProt
Manually annotated by BRENDA team
Enterococcus faecalis NBRC3971
-
UniProt
Manually annotated by BRENDA team
Streptomyces sp. OH-11242
strain OH-11242
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-
the enzyme is responsible for catalyzing the liberation of galactosyl beta-1,3-N-acetyl-D-galactosamine linked to serine or threonine residues of mucin-type glycoproteins
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc + H2O
2,4-dinitrophenol + beta-D-Gal-(1->3)-alpha-D-GalNAc
show the reaction diagram
Q8DR60, -
-
-
-
?
2,4-dinitrophenyl-GalNAc-alpha-R + H2O
2,4-dinitrophenol + GalNAc-alpha-R
show the reaction diagram
Q8DR60, -
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
-, Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
Bifidobacterium breve JCM 1192, Bifidobacterium longum JCM 7054
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
Bifidobacterium longum JCM 1217
Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
Bifidobacterium longum JCM 1217, Bifidobacterium bifidum JCM 7004, Bifidobacterium bifidum ATCC29521
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
-, Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
Bifidobacterium breve JCM 1192, Bifidobacterium longum JCM 7054
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
Bifidobacterium longum JCM 1217
Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
Bifidobacterium longum JCM 1217, Bifidobacterium bifidum JCM 7004, Bifidobacterium bifidum ATCC29521
-
-
-
-
?
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
4-nitrophenol + N-acetyl-alpha-D-galactosamine
show the reaction diagram
-, Q833X5
-
-
-
?
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
4-nitrophenol + N-acetyl-alpha-D-galactosamine
show the reaction diagram
Clostridium perfringens, Clostridium perfringens 13
Q8XMJ5
1.8% relative activity
-
-
?
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
4-nitrophenol + N-acetyl-alpha-D-galactosamine
show the reaction diagram
Enterococcus faecalis NBRC3971
Q833X5
-
-
-
?
4-nitrophenyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside + H2O
4-nitrophenol + 3-O-beta-D-galactopyranosyl-2-acetamido-2-deoxy-alpha-D-galactopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc + H2O
4-nitrophenol + beta-D-Gal-(1->3)-alpha-D-GalNAc
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc + H2O
4-nitrophenol + beta-D-Gal-(1->3)-alpha-D-GalNAc
show the reaction diagram
Q8DR60, -
-
-
-
?
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc + H2O
4-nitrophenol + beta-D-Gal-(1->3)-alpha-D-GalNAc
show the reaction diagram
Bifidobacterium longum JCM1217
-
-
-
-
?
asialo kappa-casein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
-
-
-
?
asialo kappa-casein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
-
-
-
?
asialo kappa-casein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
-
-
-
?
asialo kappa-casein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
Brevibacillus laterosporus SS-16
-
-
-
-
?
asialo kappa-casein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
Alcaligenes sp. F-1906
-
-
-
-
?
asialofetuin + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-, Q3T552
-
-
-
?
asialofetuin + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
Bifidobacterium longum JCM 1217
Q3T552
-
-
-
?
asialofetuin + H2O
?
show the reaction diagram
Clostridium perfringens, Clostridium perfringens 13
Q8XMJ5
-
-
-
?
asialoglycophorin + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
-
-
-
?
asialoglycophorin + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
-
-
-
?
asialoglycophorin + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
-
-
?
asialoglycophorin + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
asialoglycophorin A
-
-
?
asialoglycophorin + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
Brevibacillus laterosporus SS-16
-
-
-
-
?
asialoglycophorin + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
Alcaligenes sp. F-1906
-
-
-
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-butanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-butyl + p-nitrophenol
show the reaction diagram
Bacillus sp., Bacillus sp. A198
-
transfer ratio in presence of sodium cholate is 85.6%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-heptanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-heptyl + p-nitrophenol
show the reaction diagram
-
transfer ratio in presence of sodium cholate is 22.7%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-hexanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-hexyl + p-nitrophenol
show the reaction diagram
-
transfer ratio in presence of sodium cholate is 58.8%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-octanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-1-octyl + p-nitrophenol
show the reaction diagram
-
transfer ratio in presence of sodium cholate is 14.9%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-pentanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-pentyl + p-nitrophenol
show the reaction diagram
-
transfer ratio in presence of sodium cholate is 73.3%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-propanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-propyl + p-nitrophenol
show the reaction diagram
Bacillus sp., Bacillus sp. A198
-
transfer ratio in presence of sodium cholate is 76.8%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 2-benzyloxy-1-pentanol
?
show the reaction diagram
-
transfer ratio in presence of sodium cholate is 56.3%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 2-benzyloxyethanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-(2-benzyloxyethyl) + p-nitrophenol
show the reaction diagram
-
transfer ratio in presence of sodium cholate is 80.8%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + allyl alcohol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-allyl + p-nitrophenol
show the reaction diagram
-
transfer ratio in presence of sodium cholate is 86.8%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + benzyloxycarbonyl-Leu-Ser-Gln-Val-His-Arg
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-(CBZ-Leu)-Ser-Gln-Val-His-Arg + p-nitrophenol
show the reaction diagram
-
-
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + diethylene glycol monomethyl ether
?
show the reaction diagram
-
transfer ratio in presence of sodium cholate is 78.6%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + ethanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-ethyl + p-nitrophenol
show the reaction diagram
Bacillus sp., Bacillus sp. A198
-
transfer ratio in presence of sodium cholate is 65.0%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + ethylene glycol monomethyl ether
?
show the reaction diagram
-
transfer ratio in presence of sodium cholate is 79.4%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + methanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-methyl + p-nitrophenol
show the reaction diagram
Bacillus sp., Bacillus sp. A198
-
transfer ratio in presence of sodium cholate is 70.8%
-
?
dansyl-Ser-GalNAc-Gal + H2O
dansyl-Ser + GalNAc-Gal + H2O
show the reaction diagram
-
-
-
?
dansyl-Thr-GalNAc-Gal + H2O
dansyl-Thr + GalNAc-Gal + H2O
show the reaction diagram
-
-
-
?
fetuin + H2O
?
show the reaction diagram
Streptomyces sp., Streptomyces sp. OH-11242
-
hydrolysis of the O-glycosidic linkage between GalNAc and Ser or Thr
-
-
?
Gal-beta-1,3-GalNAc-alpha-1p-nitrophenol + H2O
p-nitrophenol + Gal-beta-1,3-GalNAc
show the reaction diagram
-, Q3T552
pH 5.0, 37C
-
-
?
Gal-beta-1,3-GalNAc-alpha-1p-nitrophenol + H2O
p-nitrophenol + Gal-beta-1,3-GalNAc
show the reaction diagram
Bifidobacterium longum JCM 7054
-
pH 5.0, 37C
-
-
?
Galbeta(1,3)GalNAc-asialofetuin + H2O
Galbeta(1,3)GalNAc + asialofetuin
show the reaction diagram
-, Q3T552
pH 5.0, 37C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
show the reaction diagram
-
pH 5.0, 37C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
show the reaction diagram
-, Q3T552
pH 5.0, 37C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
show the reaction diagram
-, Q3T552
pH 5.0, 37C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
show the reaction diagram
Bifidobacterium breve JCM 1192, Bifidobacterium longum JCM 1217, Bifidobacterium bifidum JCM 7004, Bifidobacterium bifidum ATCC29521
-
pH 5.0, 37C
-
-
?
Galbeta(1-3)GalNAcalpha(1-)OC6H4-o-NO2 + H2O
Galbeta(1-3)GalNAc + o-nitrophenol
show the reaction diagram
-
-
-
-
?
Galbeta(1-3)GalNAcalpha(1-)OC6H4-p-NO2 + H2O
Galbeta(1-3)GalNAc + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Galbeta(1-3)GalNAcalpha(1-)OC6H5 + H2O
Galbeta(1-3)GalNAc + phenol
show the reaction diagram
-
-
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + galactose
?
show the reaction diagram
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + glucose
?
show the reaction diagram
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + H2O
Galbeta(1-3)GalNAc + p-nitrophenol
show the reaction diagram
-
-
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + maltose
?
show the reaction diagram
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + mannitol
?
show the reaction diagram
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + mannose
?
show the reaction diagram
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + sorbitol
?
show the reaction diagram
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + sucrose
?
show the reaction diagram
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha1-p-nitrophenol + H2O
Galbeta(1-3)GalNAc + p-nitrophenol
show the reaction diagram
-, Q3T552
-
-
-
?
Galbeta1-3(GlcNAcbeta1-6)GalNAcalpha1-pNP + H2O
?
show the reaction diagram
Clostridium perfringens, Clostridium perfringens 13
Q8XMJ5
2.3% relative activity
-
-
?
Galbeta1-3GalNAcalpha-pNP + H2O
Galbeta1-3GalNAc + p-nitrophenol
show the reaction diagram
-, Q833X5
-
-
-
?
Galbeta1-3GalNAcalpha-pNP + H2O
Galbeta1-3GalNAc + p-nitrophenol
show the reaction diagram
Enterococcus faecalis NBRC3971
Q833X5
-
-
-
?
Galbeta1-3GalNAcalpha1-pNP + H2O
?
show the reaction diagram
-
100% relative activity
-
-
?
Galbeta1-3GalNAcalpha1-pNP + H2O
?
show the reaction diagram
Clostridium perfringens, Clostridium perfringens 13
Q8XMJ5
100% relative activity
-
-
?
Galbeta1-3[Galbeta1-3GlcNAcbeta1-6]GalNAcalpha-pNP + H2O
?
show the reaction diagram
-, Q833X5
-
-
-
?
Galbeta1-3[Galbeta1-3GlcNAcbeta1-6]GalNAcalpha-pNP + H2O
?
show the reaction diagram
Enterococcus faecalis NBRC3971
Q833X5
-
-
-
?
Galbeta1-3[GlcNAcbeta1-6]GalNAcalpha1-pNP + H2O
?
show the reaction diagram
-, Q833X5
-
-
-
?
Galbeta1-3[GlcNAcbeta1-6]GalNAcalpha1-pNP + H2O
?
show the reaction diagram
Enterococcus faecalis NBRC3971
Q833X5
-
-
-
?
GalNAcbeta1-3GalNAcalpha1-pNP + H2O
?
show the reaction diagram
-
17.3% relative activity
-
-
?
GalNAcbeta1-3GalNAcalpha1-pNP + H2O
?
show the reaction diagram
Q8XMJ5
21% relative activity
-
-
?
gastric mucin + H2O
disaccharide + ?
show the reaction diagram
-
EngBF does not release oligosaccharides from intact gastric mucin, but releases core 1 disaccharide from the gastric mucin pretreated with commercial sialidase and with the recombinant exo-alpha-N-acetylglucosamindase
-
-
?
gastric mucin + H2O
disaccharide + ?
show the reaction diagram
Q8XMJ5
EngCP does not release oligosaccharides from intact gastric mucin, but releases core 1 disaccharide from the gastric mucin pretreated with commercial sialidase and with the recombinant exo-alpha-N-acetylglucosamindase cloned from the same strain of Clostridium perfringens. It does not release core 2 trisaccharide from the mucin
-
-
?
Glc-NAcbeta1-3GalNAcalpha1-pNP + H2O
?
show the reaction diagram
-, Q833X5
-
-
-
?
Glcbeta1-3GalNAcalpha1-pNP + H2O
?
show the reaction diagram
Q8XMJ5
156.7% relative activity
-
-
?
Glcbeta1-3GalNAcalpha1-pNP + H2O
?
show the reaction diagram
-
35.3% relative activity
-
-
?
GlcNAcbeta1-3GalNAcalpha1-pNP + H2O
?
show the reaction diagram
-
0.3% relative activity
-
-
?
GlcNAcbeta1-3GalNAcalpha1-pNP + H2O
?
show the reaction diagram
Q8XMJ5
3.5% relative activity
-
-
?
glycopeptide + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
specific requirement for an unsubstituted galactose at the nonreducing terminus and an alpha-linkage between N-acetylgalactosamine and the aglycone
-
-
?
glycopeptide + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
glycopeptides from mouse myeloma, fetuin and pig submaxillary mucin
-
?
glycopeptide + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
release of Galbeta(1-3)GalNAc from glycopeptides possesing Ser or Thr O-glycosidic linkages
-
-
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
liberates Galbeta(1-3)4GalNAc and also other larger oligosaccharides by hydrolysis of O-glycosidic linkage between GalNAc and Ser or Thr
-
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
specific requirement for an unsubstituted galactose in the nonreducing terminus and an alpha-linkage between N-acetylgalactosamine and the aglycone
-
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
disialized human erythrocyte membrane glycoprotein, porcine submaxillarry mucin
no oligosaccharides larger than trisaccharides are liberated from porcine submaxillarry mucin
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
bovine submaxillary mucin, no hydrolysis of remodelled antifreeze glycoprotein containing NeuAcalpha2-3Galbeta1-3GalNAc and Galbeta1-3(NeuAcalpha2-6)GalNAc
-
-
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
purified pig gastric mucus glycoproteins
-
-
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
digestion of nerve cell surface glycoproteins or their terminal N-acetylgalactosamines, no digestion of galactosamines associated with the collagenous ligands or perineuronal proteoglycan
-
-
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
hydrolyzes the O-glycosidic linkage between alpha-N-acetylgalactosamine and the hydroxyl group of Ser or Thr residues in mucus and mucin-type glycoproteins
-
-
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages
-
-
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages
-
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
-
release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages
no oligosaccharides larger than trisaccharides are liberated from porcine submaxillarry mucin
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
Brevibacillus laterosporus SS-16
-
hydrolyzes the O-glycosidic linkage between alpha-N-acetylgalactosamine and the hydroxyl group of Ser or Thr residues in mucus and mucin-type glycoproteins
-
-
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
Streptomyces sp. OH-11242
-
liberates Galbeta(1-3)4GalNAc and also other larger oligosaccharides by hydrolysis of O-glycosidic linkage between GalNAc and Ser or Thr
-
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
Streptomyces sp. OH-11242
-
purified pig gastric mucus glycoproteins
-
-
?
mucin + H2O
Galbeta1-3GalNAc + ?
show the reaction diagram
-
desialylated bovine submaxillary mucin
-
?
mucin + H2O
Galbeta1-3GalNAc + ?
show the reaction diagram
Streptomyces sp., Streptomyces sp. OH-11242
-
release of Galbeta(1-3)GalNAc from mucins possesing Ser or Thr O-glycosidic linkages
-
-
?
asialo-fetuin + H2O
?
show the reaction diagram
-
almost complete removal of O-linked Galbeta1-3GalNAc, the product has a decreased trypsin inhibitory activity
-
?
asialofetuin + H2O
additional information
-
-
-
-
-
?
asialofetuin + H2O
additional information
-
-
-
-
-
?
asialofetuin + H2O
additional information
-
-
-
-
-
?
asialofetuin + H2O
additional information
-
-
-
-
-
?
asialofetuin + H2O
additional information
-
-
-
Galbeta(1-3)GalNAc + ?
?
asialofetuin + H2O
additional information
-
-
-
Galbeta(1-3)GalNAc + ?
?
asialofetuin + H2O
additional information
-
-
hydrolysis of the O-glycosidic linkage between GalNAc and Ser or Thr
Galbeta(1-3)GalNAc + Galbeta(1-3)(Galbeta(1-4)GlcNAcbeta(1-6))GalNAc
?
asialofetuin + H2O
additional information
-
Brevibacillus laterosporus SS-16
-
-
-
-
?
asialofetuin + H2O
additional information
-
Streptomyces sp. OH-11242
-
hydrolysis of the O-glycosidic linkage between GalNAc and Ser or Thr
Galbeta(1-3)GalNAc + Galbeta(1-3)(Galbeta(1-4)GlcNAcbeta(1-6))GalNAc
?
asialofetuin + H2O
additional information
-
Alcaligenes sp. F-1906
-
-
Galbeta(1-3)GalNAc + ?
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
show the reaction diagram
Alcaligenes sp. F-1906
-
release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages
-
?
additional information
?
-
-
no action on phenyl alpha-N-acetyl-D-galactosaminide, asialo ovine submaxillary mucin or monosialoganglioside
-
-
-
additional information
?
-
-
no activity when the aglycone is methanol
-
-
-
additional information
?
-
-
the enzyme also catalyzes the transfer of beta-D-Gal-(1-3)-alpha-D-GalNAc from asialofetuin to 1-alkanols
-
?
additional information
?
-
-
highly induced by porcine gastric mucin
-
-
-
additional information
?
-
-
highly induced by porcine gastric mucin
-
-
-
additional information
?
-
-
enzyme production is induced by mucin, the induction is inhibited by glucose and other easily assimilable carbon sources, as well as by complex nitrogen sources, addition of palmitate, lambda-carrageenan or crude mucin to a mucin-based production medium enhances enzyme production
-
-
-
additional information
?
-
-
induced when asialofetuin is the sole carbon source
-
-
-
additional information
?
-
-, Q3T552
Galbeta(1,3)GalNAc disaccharide is liberated from asialofetuin and p-nitrophenol substrate containing the core 1 structure (Galbeta(1,3)GalNAcalpha-1), but not from sialofetuin or any p-nitrophenol substrate other than Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
-
-
-
additional information
?
-
-, Q3T552
no reaction with Galbeta(1,3)(GlcNAcbeta(1,6))GalNAcalpha-1-p-nitrophenol, GalNAcalpha-1-p-nitrophenol, GlcNAcbeta(1,3)GalNAcalpha-1-p-nitrophenol and Galbeta(1,3)GlcNAcalpha-1-p-nitrophenol
-
-
-
additional information
?
-
-, Q3T552
the enzyme also exhibits transglycosylation activity towards various mono- and disaccharides and 1-alkanols. No activity with GalNAc-p-nitrophenol, Galbeta(1-3)(GlcNAcbeta(1-6))GalNAc-p-nitrophenol, GlcNAcbeta(1-3)GalNAc-p-nitrophenol, Galbeta(1-3)GlcNAc-p-nitrophenol. Does not release sialo-oligosaccharides from fetuin
-
-
-
additional information
?
-
B2C4H5
the expression of the gene is highly induced in the presence of mucin
-
-
-
additional information
?
-
-, Q833X5
can not release the oligosaccharide from GlcNAcbeta1-6GalNAcalpha1-pNP
-
-
-
additional information
?
-
-
EngCP from Clostridium perfringens possesses broader substrate specificity than EngBF
-
-
-
additional information
?
-
Q8XMJ5
EngCP possesses broader substrate specificity than EngBF of Bifidobacterium longum, which may contribute to the pathogenicity of Clostridium perfringens. EngCP does not act on sialyl core 1 O-glycans, e.g., fetuin
-
-
-
additional information
?
-
-
EngBF preferably releases Galbeta1-3GalNAc from the core 1-type O-glycan, i.e. Thomsen-Friedenreich antigen or T-antigen, of mucin glycoproteins. EngBF also shows transglycosylation activity of the released disaccharide to other mono- and disaccharides
-
-
-
additional information
?
-
Q8DR60, -
SpGH101 specifically removes an O-linked disaccharide Gal-beta-1,3-GalNAc-alpha from glycoproteins
-
-
-
additional information
?
-
-
the enzyme hydrolyses the O-glycosidic bonds in mucin-type O-glycan between alpha-GalNAc and Ser/Thr. EngBF is highly specific for the core 1-type O-glycan to release the disaccharide Galbeta1-3GalNAc
-
-
-
additional information
?
-
-
the enzyme is responsible for catalyzing the liberation of galactosyl beta-1,3-N-acetyl-D-galactosamine linked to serine or threonine residues of mucin-type glycoproteins
-
-
-
additional information
?
-
-
active site structure and substrate binding by the enzyme, important residues for substrate binding are Trp residues Trp748 and Trp750, appearing to form stacking interactions with the beta-faces of sugar rings of Galbeta1-3GalNAc by substrate-induced fit, substrate specificity for glycans, docking analysis, detailed overview
-
-
-
additional information
?
-
-
endo-alpha-N-acetylgalactosaminidase catalyzes the release of Galbeta1-3GalNAc from the core 1-type O-glycan, Galbeta1-3GalNAcalpha1-Ser/Thr, of mucin glycoproteins and synthetic 4-nitrophenyl alpha-linked substrates. The enzyme directly transfers Galbeta1-3GalNAc to serine or threonine residues of bioactive peptides such as PAMP-12, bradykinin, peptide-TandMUC1a when Galbeta1-3GalNAcalpha1-4NP was used as a donor substrate. The enzyme also catalyzes the reverse-hydrolysis reaction. EngBF synthesizes the core 1 disaccharide-containing oligosaccharides when the enzyme is incubated with either glucose or lactose and Galbeta1-3GalNAc
-
-
-
additional information
?
-
Q8DR60, -
the enzyme contains several putative carbohydrate binding modules, structure-function relationship, D764 and E796 are the nucleophile and general acid-base residues, respectively, overview
-
-
-
additional information
?
-
Bifidobacterium longum JCM 1217
Q3T552
no reaction with Galbeta(1,3)(GlcNAcbeta(1,6))GalNAcalpha-1-p-nitrophenol, GalNAcalpha-1-p-nitrophenol, GlcNAcbeta(1,3)GalNAcalpha-1-p-nitrophenol and Galbeta(1,3)GlcNAcalpha-1-p-nitrophenol, the enzyme also exhibits transglycosylation activity towards various mono- and disaccharides and 1-alkanols. No activity with GalNAc-p-nitrophenol, Galbeta(1-3)(GlcNAcbeta(1-6))GalNAc-p-nitrophenol, GlcNAcbeta(1-3)GalNAc-p-nitrophenol, Galbeta(1-3)GlcNAc-p-nitrophenol. Does not release sialo-oligosaccharides from fetuin
-
-
-
additional information
?
-
Bifidobacterium longum JCM 1217
-
Galbeta(1,3)GalNAc disaccharide is liberated from asialofetuin and p-nitrophenol substrate containing the core 1 structure (Galbeta(1,3)GalNAcalpha-1), but not from sialofetuin or any p-nitrophenol substrate other than Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
-
-
-
additional information
?
-
Clostridium perfringens 13
Q8XMJ5
EngCP possesses broader substrate specificity than EngBF of Bifidobacterium longum, which may contribute to the pathogenicity of Clostridium perfringens. EngCP does not act on sialyl core 1 O-glycans, e.g., fetuin
-
-
-
additional information
?
-
Enterococcus faecalis NBRC3971
Q833X5
can not release the oligosaccharide from GlcNAcbeta1-6GalNAcalpha1-pNP
-
-
-
additional information
?
-
Bacillus sp. A198
-
the enzyme also catalyzes the transfer of beta-D-Gal-(1-3)-alpha-D-GalNAc from asialofetuin to 1-alkanols
-
?
additional information
?
-
Bifidobacterium longum JCM1217
-
EngBF preferably releases Galbeta1-3GalNAc from the core 1-type O-glycan, i.e. Thomsen-Friedenreich antigen or T-antigen, of mucin glycoproteins. EngBF also shows transglycosylation activity of the released disaccharide to other mono- and disaccharides, endo-alpha-N-acetylgalactosaminidase catalyzes the release of Galbeta1-3GalNAc from the core 1-type O-glycan, Galbeta1-3GalNAcalpha1-Ser/Thr, of mucin glycoproteins and synthetic 4-nitrophenyl alpha-linked substrates. The enzyme directly transfers Galbeta1-3GalNAc to serine or threonine residues of bioactive peptides such as PAMP-12, bradykinin, peptide-TandMUC1a when Galbeta1-3GalNAcalpha1-4NP was used as a donor substrate. The enzyme also catalyzes the reverse-hydrolysis reaction. EngBF synthesizes the core 1 disaccharide-containing oligosaccharides when the enzyme is incubated with either glucose or lactose and Galbeta1-3GalNAc
-
-
-
additional information
?
-
Streptomyces sp. OH-11242
-
highly induced by porcine gastric mucin
-
-
-
additional information
?
-
Streptomyces sp. OH-11242
-
enzyme production is induced by mucin, the induction is inhibited by glucose and other easily assimilable carbon sources, as well as by complex nitrogen sources, addition of palmitate, lambda-carrageenan or crude mucin to a mucin-based production medium enhances enzyme production
-
-
-
additional information
?
-
Alcaligenes sp. F-1906
-
highly induced by porcine gastric mucin
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
-, Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
Bifidobacterium breve JCM 1192, Bifidobacterium longum JCM 7054
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
Bifidobacterium longum JCM 1217
Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
show the reaction diagram
Bifidobacterium longum JCM 1217, Bifidobacterium bifidum JCM 7004, Bifidobacterium bifidum ATCC29521
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
-, Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
Bifidobacterium breve JCM 1192, Bifidobacterium longum JCM 7054
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
Bifidobacterium longum JCM 1217
Q3T552
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
show the reaction diagram
Bifidobacterium longum JCM 1217, Bifidobacterium bifidum JCM 7004, Bifidobacterium bifidum ATCC29521
-
-
-
-
?
additional information
?
-
-
highly induced by porcine gastric mucin
-
-
-
additional information
?
-
-
highly induced by porcine gastric mucin
-
-
-
additional information
?
-
-
enzyme production is induced by mucin, the induction is inhibited by glucose and other easily assimilable carbon sources, as well as by complex nitrogen sources, addition of palmitate, lambda-carrageenan or crude mucin to a mucin-based production medium enhances enzyme production
-
-
-
additional information
?
-
-
induced when asialofetuin is the sole carbon source
-
-
-
additional information
?
-
B2C4H5
the expression of the gene is highly induced in the presence of mucin
-
-
-
additional information
?
-
-
EngBF preferably releases Galbeta1-3GalNAc from the core 1-type O-glycan, i.e. Thomsen-Friedenreich antigen or T-antigen, of mucin glycoproteins. EngBF also shows transglycosylation activity of the released disaccharide to other mono- and disaccharides
-
-
-
additional information
?
-
Q8DR60, -
SpGH101 specifically removes an O-linked disaccharide Gal-beta-1,3-GalNAc-alpha from glycoproteins
-
-
-
additional information
?
-
-
the enzyme hydrolyses the O-glycosidic bonds in mucin-type O-glycan between alpha-GalNAc and Ser/Thr. EngBF is highly specific for the core 1-type O-glycan to release the disaccharide Galbeta1-3GalNAc
-
-
-
additional information
?
-
-
the enzyme is responsible for catalyzing the liberation of galactosyl beta-1,3-N-acetyl-D-galactosamine linked to serine or threonine residues of mucin-type glycoproteins
-
-
-
additional information
?
-
Bifidobacterium longum JCM1217
-
EngBF preferably releases Galbeta1-3GalNAc from the core 1-type O-glycan, i.e. Thomsen-Friedenreich antigen or T-antigen, of mucin glycoproteins. EngBF also shows transglycosylation activity of the released disaccharide to other mono- and disaccharides
-
-
-
additional information
?
-
Streptomyces sp. OH-11242
-
highly induced by porcine gastric mucin
-
-
-
additional information
?
-
Streptomyces sp. OH-11242
-
enzyme production is induced by mucin, the induction is inhibited by glucose and other easily assimilable carbon sources, as well as by complex nitrogen sources, addition of palmitate, lambda-carrageenan or crude mucin to a mucin-based production medium enhances enzyme production
-
-
-
additional information
?
-
Alcaligenes sp. F-1906
-
highly induced by porcine gastric mucin
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
at a level twice the EDTA concentration completely restores enzyme activity
Co2+
-
activates at 5 mM
Mg2+
-
at a level twice the EDTA concentration completely restores enzyme activity
Mg2+
-
activates at 5 mM
Mn2+
-
activates at 5 mM
NaCl
Q8DR60, -
effects on kinetics, overview
NaN3
Q8DR60, -
effects on kinetics, overview
Ni2+
-
activates at 5 mM
Sodium formate
Q8DR60, -
effects on kinetics, overview
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Ag+
-
5 mM, 35% inhibition
C2H5HgCl
-
5 mM, 23% inhibition
Ca2+
-
2.5 mM CaCl2, 3.3% inhibition
Ca2+
-
5 mM, 26% inhibition
Cd2+
-
5 mM, 22% inhibition
Cd2+
-
strong inhibition at 5 mM
CH3HgCl
-
5 mM, 18% inhibition
Co2+
-
5 mM, 18% inhibition
Cr3+
-
5 mM, 58% inhibition
Cu2+
-
2.5 mM, 26% inhibition
Cu2+
-
5 mM, 20% inhibition
Cu2+
-, Q833X5
1 mM greatly inhibits
Cu2+
Q8XMJ5
is severely inhibited by 1 mM
Cys
-
2 mM, 12% inhibition
Cys
-
2.5 mM, 32% inhibition
DFP
-
1 mM, 48% inhibition
EDTA
-
2 mM, 40% inhibition
EDTA
-
2.5 mM, 63% inhibition
Fe2+
-
1 mM
-
Fe3+
-
5 mM, 15% inhibition
-
Galactonolactone
-
5 mM, 11% inhibition
glucose
-
5 mM, 11% inhibition
Hg2+
-
2 mM, complete inhibition
Hg2+
-
2.5 mM, complete inhibition
Hg2+
-
5 mM, 20% inhibition
iodoacetate
-
5 mM, 17% inhibition
L-Cys
-
2 mM, 12% inhibition
Mg2+
-
2.5 mM, 2.2% inhibition
Mg2+
-
2 mM, 28% inhibition
Mg2+
-
5 mM, 17% inhibition
Mn2+
-
2 mM, 29% inhibition
Mn2+
-
2.5 mM, 35% inhibition
Mn2+
-
2.5 mM, 44% inhibition
N-bromosuccinimide
-
1 mM, complete inhibition
p-chloromercuribenzenesulfonate
-
1 mM, 60% inhibition
p-Nitrophenyl-2-acetamido-2-deoxy-3-O-beta-D-galactopyranosyl-beta-D-galactopyranoside
-
-
PCMB
-
1 mM, 26% inhibition
SDS
-
strong inhibition
Sodium deoxycholate
-
slightly effective
Triton X-100
-
slightly effective
Tween 20
-
slightly effective
Tween 80
-
slightly effective
Zn2+
-
2.5 mM, 66% inhibition
Zn2+
-
5 mM, 9% inhibition
Zn2+
-
strong inhibition at 5 mM
Mn2+
-
5 mM, 22% inhibition
additional information
-, Q833X5
1 mM of Mg2+, Ni2+, Ca2+, Co2+, Na+, Mn2+, Fe2+, Fe3+, EGTA, and EDTA have little effect on the enzyme activity
-
additional information
-
EngBF is not significantly inhibited by divalent cations
-
additional information
Q8XMJ5
1 mM of Ca2+, Zn2+, Co2+, Mn2+, Mg2+, and Fe2+, or the chelating reagent EDTA have no effect on activity
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0012
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796Q
0.0013
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796A
0.033
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant D764A
0.034
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, DELTA1-39 deletion mutant
0.048
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, DELTA1-39,DELTA1568-1767 deletion mutant
2.1
-
2,4-dinitrophenyl-GalNAc-alpha-R
Q8DR60, -
pH 6.5, 37C, mutant E796A
-
2.4
-
2,4-dinitrophenyl-GalNAc-alpha-R
Q8DR60, -
pH 6.5, 37C, DELTA1-39 deletion mutant
-
0.021
-
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796A
0.062
-
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, DELTA1-39 deletion mutant
0.212
-
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796Q
3.2
-
asialo kappa-casein
-
-
-
0.78
-
asialo-kappa-casein
-
-
-
0.52
-
asialofetuin
-
-
-
1
-
asialofetuin
-
-
-
3.7
-
asialofetuin
-
-
-
0.32
-
asialoglycophorin
-
-
-
0.17
-
dansyl-Ser-GalNAc-Gal
-
-
1.43
-
dansyl-Thr-GalNAc-Gal
-
-
0.0218
-
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
Q3T552
wild-type, pH 5.0, 37C
0.0384
-
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
Q3T552
mutant D762A, pH 5.0, 37C
0.107
-
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
Q3T552
mutant E822A, pH 5.0, 37C
0.165
-
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
-, Q3T552
pH 5.0, 37C
0.23
-
Galbeta(1-3)GalNAcalpha(1-)OC6H4-o-NO2
-
-
0.25
-
Galbeta(1-3)GalNAcalpha(1-)OC6H4-p-NO2
-
-
0.0218
-
Galbeta(1-3)GalNAcalpha1-p-nitrophenol
Q3T552
wild-type enzyme
0.0384
-
Galbeta(1-3)GalNAcalpha1-p-nitrophenol
Q3T552
mutant enzyme D762A
0.107
-
Galbeta(1-3)GalNAcalpha1-p-nitrophenol
Q3T552
mutant enzyme E822A
0.0596
-
Galbeta1-3GalNAcalpha-pNP
-, Q833X5
-
0.051
-
Galbeta1-3GalNAcalpha1-pNP
Q8XMJ5
-
additional information
-
additional information
-
Km for glycopeptides obtained by trypsin digestion of human erythrocyte membrane
-
additional information
-
additional information
-
-
-
additional information
-
additional information
Q8DR60, -
kinetic analysis, overview
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.76
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant D764A
14
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796Q
18
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796A
541
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, DELTA1-39 deletion mutant
748
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, DELTA1-39,DELTA1568-1767 deletion mutant
1.6
-
2,4-dinitrophenyl-GalNAc-alpha-R
Q8DR60, -
pH 6.5, 37C, DELTA1-39 deletion mutant
-
5
-
2,4-dinitrophenyl-GalNAc-alpha-R
Q8DR60, -
pH 6.5, 37C, mutant E796A
-
1
-
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796A
2.1
-
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796Q
579
-
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, DELTA1-39 deletion mutant
0.23
-
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
Q3T552
mutant E822A, pH 5.0, 37C
8.17
-
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
Q3T552
mutant D762A, pH 5.0, 37C
17.8
-
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
Q3T552
pH 5.0, 37C
37
-
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
-, Q3T552
pH 5.0, 37C
0.23
-
Galbeta(1-3)GalNAcalpha1-p-nitrophenol
Q3T552
mutant enzyme E822A
8.17
-
Galbeta(1-3)GalNAcalpha1-p-nitrophenol
Q3T552
mutant enzyme D762A
17.8
-
Galbeta(1-3)GalNAcalpha1-p-nitrophenol
Q3T552
wild-type enzyme
49.9
-
Galbeta1-3GalNAcalpha-pNP
-, Q833X5
-
110
-
Galbeta1-3GalNAcalpha1-pNP
Q8XMJ5
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.023
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant D764A
302425
0.987
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant D658A
302425
11.7
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796Q
302425
13.8
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796A
302425
15.6
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, DELTA1-39,DELTA1568-1767 deletion mutant
302425
15.9
-
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, DELTA1-39 deletion mutant
302425
0.0006
-
2,4-dinitrophenyl-GalNAc-alpha-R
Q8DR60, -
pH 6.5, 37C, DELTA1-39 deletion mutant
0
0.002
-
2,4-dinitrophenyl-GalNAc-alpha-R
Q8DR60, -
pH 6.5, 37C, mutant E796A
0
0.0099
-
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796Q
302427
0.048
-
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, mutant E796A
302427
9.3
-
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc
Q8DR60, -
pH 6.5, 37C, DELTA1-39 deletion mutant
302427
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2.18
-
-
-
30.1
-
-
-
additional information
-
-
-
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.5
6
-
hydrolysis of dansyl-Thr-GalNAc-Gal
4.5
7.5
-
hydrolysis of dansyl-Ser-GalNAc-Gal
5
-
-, Q3T552
at 37C in 10 mM acetate buffer
6
-
-, Q833X5
-
6
-
Q8XMJ5
optimum pH of the enzyme for Galbeta1-3GalNAcalpha1-pNP hydrolysis is at pH 6.0 and 9.0, whereas optimum pH for other substrates hydrolyzed is around pH 6.0
6.5
-
Q8DR60, -
assay at
additional information
-
-
optimal pH between pH 5-6 for all hydrolyzable substrates of EngBF
additional information
-
Q8DR60, -
bell-shaped dependence of kcat/Km upon pH
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
6
-, Q3T552
68% activity at pH 4.0, 73% activity at pH 6.0
4
9
-
about 50% of maximal activity at pH 4.0 and at pH 9.0
5.5
7
-
about 50% of maximal activity at pH 5.5 and at pH 7.0
6
8.8
-
pH 6.0: about 40% of maximal activity, pH 8.8: about 60% of maximal activity
6
9
Q8XMJ5
retains more than 80% activity
additional information
-
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
Q8DR60, -
assay at
37
-
-
assay at
45
-
-, Q833X5
-
60
-
-, Q3T552
-
60
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
extracellular matrix
Manually annotated by BRENDA team
Alcaligenes sp. F-1906, Streptomyces sp. OH-11242
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Bifidobacterium longum JCM 1217
-
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
105000
-
-
gel filtration
110000
-
-
gel filtration
160000
-
-
gel filtration
160000
-
-
gel filtration
200000
-
Q3T552
gel filtration
210000
-
-
gel filtration
210000
-
-, Q3T552
predicted from amino acid sequence
210300
-
Q3T552
calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 190000, SDS-PAGE
?
Q3T552
x * 200000, SDS-PAGE; x * 210269, calculated from sequence
?
-, Q3T552
x * 210000, SDS-PAGE
?
Bifidobacterium longum JCM 1217
-
x * 200000, SDS-PAGE; x * 210000, SDS-PAGE; x * 210269, calculated from sequence
-
monomer
-
1 * 160000, SDS-PAGE
monomer
-
1 * 110000, SDS-PAGE
monomer
Q3T552
1 * 200000, SDS-PAGE, gel filtration
monomer
-, Q833X5
1 * 150000, SDS-PAGE
monomer
Alcaligenes sp. F-1906
-
1 * 160000, SDS-PAGE
-
monomer
Bifidobacterium longum JCM 1217
-
1 * 200000, SDS-PAGE, gel filtration
-
tetramer
-
4 * 48000, SDS-PAGE
tetramer
Brevibacillus laterosporus SS-16
-
4 * 48000, SDS-PAGE
-
monomer
Enterococcus faecalis NBRC3971
-
1 * 150000, SDS-PAGE
-
additional information
Q8DR60, -
analysis of the three-dimensional structure of the recombinant protein, the enzyme contains several putative carbohydrate binding modules, structure-function relationship, overview
additional information
-
docking analysis and structural model building, overview
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
purified recombinant His-tagged enzyme, X-ray diffraction structure analysis at 2.0-2.5 A resolution
-
purified recombinant isolated catalytic domain, hanging drop vapour diffusion method at 19C, 1 ml of 15 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with 1 ml 25% PEG 1500. Removal of the His6-tag is unnecessary for crystallization. Crystals are cryoprotected in 1 ml 33% PEG 1500 supplemented with 6% MPD, X-ray diffraction structure determination and analysis at 2.0 A resolution
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.5
9
-
37C, 1 h, stable
4
10
-
37C, 30 min, stable
4.5
6.5
-
30C, 1 h, stable
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35
-
-
10 min, stable below
37
-
Q3T552
stable up to; the enzyme is stable up to 37C and retains 73% activity after incubation at 45C for 30 min in 50 mM acetate buffer (pH 5.0)
37
-
-, Q3T552
the enzyme is stable up to 37C and retains 73% activity after incubation at 45C for 30 min in 50 mM acetate buffer (pH 5.0)
45
-
Q3T552
pH 5.0, 30 min, enzyme retains 73% of initial activity
55
-
-
10 min, complete inactivation
55
-
-
1 h, stable below
55
-
-
10 min, stable
70
-
-
1 h, complete inactivation
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, 2 months, stable
-
-20C, stable for 3 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
; recombinant
Q3T552
recombinant enzyme
-, Q3T552
recombinant His-tagged EngBF from Escherichia coli strain BL21(DE3) by affinity chromatography and gel filtration
-
recombinant His-tagged EngBF truncation and and point mutants from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration
-
by gel filtration
-, Q833X5
recombinant wild-type and mutant enzymes from Escherichia coli strain AD202 by anion exchange chromatography, ultrafiltration, gel filtration, and hydrophobic interaction chromatography
Q8DR60, -
recombinant isolated catalytic domain from Escherichia coli strain BL21 Star (DE3)
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-, Q3T552
expression in Escherichia coli BL21; expression in Escherichia coli, wild-type and mutants D601N, D605N, D612N, D655N, D665N, E679Q, D682N, D682A, D700N, E723Q, D762N, D762A, D789N, D789A, E822Q, E822A, E828Q, D839N, D864N, D898N, D1058N, D1106N, D1248N, E1276Q, E1302Q, E1350Q
Q3T552
expression of His-tagged EngBF in Escherichia coli strain BL21(DE3)
-
expression of His-tagged truncated EngBF mutants, comprising residues 340-1528 and 340-1694, respectively, and point mutants in Escherichia coli strain BL21(DE3)
-
EngCP expressed in Escherichia coli
Q8XMJ5
into vector pET23a and expressed with a histidine-tag in Escherichia coli BL21(DE3)
-, Q833X5
expression of wild-type and mutant enzymes in Escherichia coli strain AD202
Q8DR60, -
expression of the isolated catalytic domain in Escherichia coli strain BL21 Star (DE3)
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D1058N
Q3T552
activity in cell-free extracts of Escherichia coli almost comparable to wild-type
D1106N
Q3T552
10-30% activity compared to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit 10-30% of the wild-type endo-alpha-GalNAc activity
D1248N
Q3T552
10-30% activity compared to wild-type
D1295A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
D601N
Q3T552
activity in cell-free extracts of Escherichia coli almost comparable to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit endo-alpha-GalNAc activity almost comparable with that of the cells carrying wild-type
D605N
Q3T552
10-30% activity compared to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit 10-30% of the wild-type endo-alpha-GalNAc activity
D612N
Q3T552
activity in cell-free extracts of Escherichia coli almost comparable to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit endo-alpha-GalNAc activity almost comparable with that of the cells carrying wild-type
D655N
Q3T552
10-30% activity compared to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit 10-30% of the wild-type endo-alpha-GalNAc activity
D665N
Q3T552
activity in cell-free extracts of Escherichia coli almost comparable to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit endo-alpha-GalNAc activity almost comparable with that of the cells carrying wild-type
D682A
Q3T552
no activity
D682A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the truncated wild-type enzyme
D682N
Q3T552
Escherichia coli cells expressing the mutant enzyme exhibit less than 2% of the wild-type endo-alpha-GalNAc activity; significant decrease in activity (below 2%)
D700N
Q3T552
activity in cell-free extracts of Escherichia coli almost comparable to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit endo-alpha-GalNAc activity almost comparable with that of the cells carrying wild-type
D762A
Q3T552
activity almost comparable to wild-type; kcat/KM is 3.9fold lower than wild-type value
D762N
Q3T552
Escherichia coli cells expressing the mutant enzyme exhibit less than 2% of the wild-type endo-alpha-GalNAc activity; significant decrease in activity (below 2%)
D789A
Q3T552
no activity
D789A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the truncated wild-type enzyme
D789N
Q3T552
Escherichia coli cells expressing the mutant enzyme exhibit less than 2% of the wild-type endo-alpha-GalNAc activity; significant decrease in activity (below 2%)
D839N
Q3T552
activity in cell-free extracts of Escherichia coli almost comparable to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit endo-alpha-GalNAc activity almost comparable with that of the cells carrying wild-type
D864N
Q3T552
activity in cell-free extracts of Escherichia coli almost comparable to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit endo-alpha-GalNAc activity almost comparable with that of the cells carrying wild-type
D898N
Q3T552
activity in cell-free extracts of Escherichia coli almost comparable to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit endo-alpha-GalNAc activity almost comparable with that of the cells carrying wild-type
E1276Q
Q3T552
10-30% activity compared to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit 10-30% of the wild-type endo-alpha-GalNAc activity
E1302Q
Q3T552
activity in cell-free extracts of Escherichia coli almost comparable to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit endo-alpha-GalNAc activity almost comparable with that of the cells carrying wild-type
E1350Q
Q3T552
activity in cell-free extracts of Escherichia coli almost comparable to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit endo-alpha-GalNAc activity almost comparable with that of the cells carrying wild-type
E679Q
Q3T552
activity in cell-free extracts of Escherichia coli almost comparable to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit endo-alpha-GalNAc activity almost comparable with that of the cells carrying wild-type
E723Q
Q3T552
10-30% activity compared to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit 10-30% of the wild-type endo-alpha-GalNAc activity
E822A
Q3T552
kcat/KM is 380fold lower than wild-type value; significant decrease in kcat and slight increase in Km
E822A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
E822Q
Q3T552
significant decrease in activity (below 2%)
E828Q
Q3T552
10-30% activity compared to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit 10-30% of the wild-type endo-alpha-GalNAc activity
E882Q
Q3T552
Escherichia coli cells expressing the mutant enzyme exhibit less than 2% of the wild-type endo-alpha-GalNAc activity
K1199A
-
site-directed mutagenesis, the mutant shows increased activity compared to the truncated wild-type enzyme
N720A
-
site-directed mutagenesis, the mutant shows increased activity compared to the truncated wild-type enzyme
Q894A
-
site-directed mutagenesis, the mutant shows increased activity compared to the truncated wild-type enzyme
S1248N
Q3T552
Escherichia coli cells expressing the mutant enzyme exhibit 10-30% of the wild-type endo-alpha-GalNAc activity
W748A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
W748F
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
W748Y
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
W750A
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
W750F
-
site-directed mutagenesis, the mutant shows reduced activity compared to the truncated wild-type enzyme
W750Y
-
site-directed mutagenesis, the mutant shows reduced activity compared to the truncated wild-type enzyme
Y787F
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the truncated wild-type enzyme
D601N
Bifidobacterium longum JCM 1217
-
activity in cell-free extracts of Escherichia coli almost comparable to wild-type; Escherichia coli cells expressing the mutant enzyme exhibit endo-alpha-GalNAc activity almost comparable with that of the cells carrying wild-type
-
D612N
Bifidobacterium longum JCM 1217
-
activity in cell-free extracts of Escherichia coli almost comparable to wild-type
-
D762N
Bifidobacterium longum JCM 1217
-
Escherichia coli cells expressing the mutant enzyme exhibit less than 2% of the wild-type endo-alpha-GalNAc activity; significant decrease in activity (below 2%)
-
D658A
Q8DR60, -
site-directed mutagenesis, the substrate specificity and activity is altered compared to the wild-type enzyme
D764A
Q8DR60, -
site-directed mutagenesis, the substrate specificity and activity is altered compared to the wild-type enzyme
D764F
Q8DR60, -
site-directed mutagenesis, the substrate specificity and activity is altered compared to the wild-type enzyme
E796A
Q8DR60, -
site-directed mutagenesis, the substrate specificity and activity is altered compared to the wild-type enzyme
E796Q
Q8DR60, -
site-directed mutagenesis, the substrate specificity and activity is altered compared to the wild-type enzyme
additional information
Q8DR60, -
construction of a DELTA1-39 deletion and DELTA1-39,DELTA1568-1767 deletion mutants
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
-
the enzyme can release T-antigen, Galbeta(1-3)GalNAc, a carcinoma-associated marker from human cells, enzyme is useful for detection and determination of T-antigen from human erythrocytes and native human gastric carcinoma cells
synthesis
-
production of Galbeta(1-3)GalNAc from asialofetuin
analysis
-
the enzyme is ideally suited to explore the distribution and function of mucin-type glycoproteins on normal and cancer cell surfaces
analysis
-
the enzyme is useful in the study of the structures of the carbohydrate moieties of various complex carbohydrates
synthesis
-
synthesis of a wide variety of O-linked glycopeptides