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3.2.1.97: endo-alpha-N-acetylgalactosaminidase

This is an abbreviated version!
For detailed information about endo-alpha-N-acetylgalactosaminidase, go to the full flat file.

Word Map on EC 3.2.1.97

Reaction

beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-[glycoprotein]-L-serine/L-threonine
+
H2O
=
beta-D-galactosyl-(1->3)-N-acetyl-D-galactosamine
 +
[glycoprotein]-L-serine/L-threonine

Synonyms

acetylgalactosaminidase, endo-alpha, D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetylgalactosaminohydrolase, EC 3.2.1.110, endo GalNAc-ase S, endo-alpha-acetylgalactosaminidase, endo-alpha-GalNAc-ase, endo-alpha-N-acetylgalactosaminidase, endo-alpha-N-acetylgalactosaminidase S, endo-beta-N-acetylgalactosaminidase, endo-EF, endo-GalNAc-ase S, endoEF, EngBF, EngCP, More, NagBb, SpGH101

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.97 endo-alpha-N-acetylgalactosaminidase

Purification

Purification on EC 3.2.1.97 - endo-alpha-N-acetylgalactosaminidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by gel filtration
HisTrap column chromatography and Superdex 200 gel filtration
partial
recombinant enzyme
recombinant His-tagged EngBF from Escherichia coli strain BL21(DE3) by affinity chromatography and gel filtration
-
recombinant His-tagged EngBF truncation and point mutants from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration
-
recombinant isolated catalytic domain from Escherichia coli strain BL21 Star (DE3)
-
recombinant wild-type and mutant enzymes from Escherichia coli strain AD202 by anion exchange chromatography, ultrafiltration, gel filtration, and hydrophobic interaction chromatography