3.2.1.182: 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase
This is an abbreviated version!
For detailed information about 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase, go to the full flat file.
Reaction
Synonyms
benzoxazinone-glucoside beta-D-glucosidase, beta-glu, beta-glucosidase, Co-Glu, DIMBOA glucosidase, DIMBOAGlc hydrolase, GDIBOA-glucosidase, LgGLU1, LgGLU2, LgGLU3, LgGLU4, More, SbDhr1, ScGlu, TaGlu1, TaGlu1a, TaGlu1b, TaGlu1c, ZmGlu1
ECTree
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Purification
Purification on EC 3.2.1.182 - 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase
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using HisTrap HP column and and Superdex 200 HR
using isoelectric precipitation, anion exchange chromatography, and gel filtration
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using Ni-NTA chromatography