3.2.1.182: 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase
This is an abbreviated version!
For detailed information about 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase, go to the full flat file.
Word Map on EC 3.2.1.182
Reaction
Synonyms
benzoxazinone-glucoside beta-D-glucosidase, beta-glu, beta-glucosidase, Co-Glu, DIMBOA glucosidase, DIMBOAGlc hydrolase, GDIBOA-glucosidase, LgGLU1, LgGLU2, LgGLU3, LgGLU4, More, SbDhr1, ScGlu, TaGlu1, TaGlu1a, TaGlu1b, TaGlu1c, ZmGlu1
ECTree
3.2.1.182 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.1.182 - 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one beta-D-glucopyranoside + H2O
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose
-
the maximum activity with DIMBOA-Glc is 10times higher in shoots and 7times higher in roots than that with DIBOA-Glc
-
-
?
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one beta-D-glucopyranoside + H2O
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose
-
the maximum activity with DIMBOA-Glc is 10times higher in shoots and 7times higher in roots than that with DIBOA-Glc
-
-
?
2-hydroxy-1,4-benzoxazin-3-one-beta-D-glucoside + H2O
2-hydroxy-1,4-benzoxazin-3-one + beta-D-glucose
-
-
-
-
?
2-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose
-
-
-
-
?
4-hydroxy-3-oxo-3,4,dihydro-2H-1,4,benzoxazin-2-yl beta-D-gluco-pyranoside + H2O
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose
-
-
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O
4-methylumbelliferone + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl-beta-D-cellobioside + H2O
4-nitrophenol + D-cellobiose
-
-
-
?
4-nitrophenyl-beta-D-fucopyranoside + H2O
4-nitrophenol + D-fucopyranose
-
-
-
?
4-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
?
7-methoxy-2,4-dihydroxy-1,4-benzoxazin-3-one beta-D-glucoside + H2O
2-hydroxy-7-methoxy-3,4-dihydro-2H-1,4-benzoxazin + beta-D-glucose
i.e. benzoxazinoid GDIMBOA, a DIBOA glucoside
-
-
?
7-methoxy-3-oxo-3,4-dihydro-2H-1,4 benzoxazin-2-yl beta-D-glucopyranoside + H2O
2-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4 benzoxazin + beta-D-glucose
-
-
-
-
?
DIBOA-beta-D-glucoside + H2O
DIBOA + beta-D-glucose
-
TaGlu1b hydrolyzes DIMBOA-Glc much better than DIBOA-Glc
-
-
?
DIMBOA-beta-D-glucoside + H2O
DIMBOA + beta-D-glucose
-
TaGlu1b hydrolyzes DIMBOA-Glc much better than DIBOA-Glc
-
-
?
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
-
-
?
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
preferred substrate
-
-
?
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
-
-
?
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
-
-
?
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
low activity
-
-
?
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
-
-
?
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-ol + D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-fucoside + H2O
4-nitrophenol + beta-D-fucose
-
-
-
-
?
4-nitrophenyl beta-D-fucoside + H2O
4-nitrophenol + beta-D-fucose
-
-
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
-
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
?
4-nitrophenyl beta-D-xyloside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
-
?
DIBOA beta-D-glucoside + H2O
DIBOA + beta-D-glucose
i.e. benzoxazinoid GDIBOA, a DIBOA glucoside
-
-
?
DIBOA-glucoside + H2O
DIBOA + beta-D-glucose
ScGlu shows similar enzyme activity toward DIMBOA-Glc and DIBOA-Glc
-
-
?
DIMBOA-glucoside + H2O
DIMBOA + beta-D-glucose
ScGlu shows similar enzyme activity toward DIMBOA-Glc and DIBOA-Glc
-
-
?
additional information
?
-
GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
-
GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and genistin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, geniposide, trans-zeatin-O-glucoside, phloridzin, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and genistin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, geniposide, trans-zeatin-O-glucoside, phloridzin, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and genistin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, geniposide, trans-zeatin-O-glucoside, phloridzin, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and genistin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, geniposide, trans-zeatin-O-glucoside, phloridzin, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and genistin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, geniposide, trans-zeatin-O-glucoside, phloridzin, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and phloridzin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, genistin, geniposide, trans-zeatin-O-glucoside, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and phloridzin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, genistin, geniposide, trans-zeatin-O-glucoside, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and phloridzin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, genistin, geniposide, trans-zeatin-O-glucoside, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and phloridzin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, genistin, geniposide, trans-zeatin-O-glucoside, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides benzoxazinoid GDIBOA, the enzyme catalyzes the hydrolsis of oleuropein, dhurrin, daidzin, and phloridzin. The enzyme does not catalyze the hydrolysis of benzoxazinoid GDIMBOA, harpagide, arbutin, genistin, geniposide, trans-zeatin-O-glucoside, and 4-nitrophenyl-beta-D-mannopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides the benzoxazinoids, the enzyme catalyzes the hydrolsis of oleuropein, geniposide, dhurrin, daidzin, phloridzin, genistin, and trans-zeatin-O-glucoside. The enzyme does not catalyze the hydrolysis of harpagide, arbutin, 4-nitrophenyl-beta-D-mannopyranoside, and 4-nitrophenyl-beta-D-fucopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides the benzoxazinoids, the enzyme catalyzes the hydrolsis of oleuropein, geniposide, dhurrin, daidzin, phloridzin, genistin, and trans-zeatin-O-glucoside. The enzyme does not catalyze the hydrolysis of harpagide, arbutin, 4-nitrophenyl-beta-D-mannopyranoside, and 4-nitrophenyl-beta-D-fucopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides the benzoxazinoids, the enzyme catalyzes the hydrolsis of oleuropein, geniposide, dhurrin, daidzin, phloridzin, genistin, and trans-zeatin-O-glucoside. The enzyme does not catalyze the hydrolysis of harpagide, arbutin, 4-nitrophenyl-beta-D-mannopyranoside, and 4-nitrophenyl-beta-D-fucopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides the benzoxazinoids, the enzyme catalyzes the hydrolsis of oleuropein, geniposide, dhurrin, daidzin, phloridzin, genistin, and trans-zeatin-O-glucoside. The enzyme does not catalyze the hydrolysis of harpagide, arbutin, 4-nitrophenyl-beta-D-mannopyranoside, and 4-nitrophenyl-beta-D-fucopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
-
-
?
additional information
?
-
-
substrate specificity of LgGLU isozymes with different substrates: artificial p-nitrophenyl glycoside substrates, benzoxazinoids, iridoids, cyanogenic glycoside, isoflavonoid glucosides, hydroquinone glucoside, and cytokinin glucoside, overview. Besides the benzoxazinoids, the enzyme catalyzes the hydrolsis of oleuropein, geniposide, dhurrin, daidzin, phloridzin, genistin, and trans-zeatin-O-glucoside. The enzyme does not catalyze the hydrolysis of harpagide, arbutin, 4-nitrophenyl-beta-D-mannopyranoside, and 4-nitrophenyl-beta-D-fucopyranoside. GDIBOA BGLUs of the dicots are promiscuous enzymes
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-
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