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Literature summary for 3.2.1.182 extracted from
- Purification and characterization of a beta-glucosidase from rye (Secale cereale L.) seedlings (2000), Plant sci., 155, 67-74.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| castanospermine | - |
Secale cereale |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.616 | - |
4-nitrophenyl beta-D-fucoside | pH 5.5, 25°C | Secale cereale | |
| 0.617 | - |
DIMBOA-glucoside | pH 5.5, 25°C | Secale cereale | |
| 0.89 | - |
2-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside | pH 5.5, 25°C | Secale cereale | |
| 0.9 | - |
4-nitrophenyl beta-D-glucoside | pH 5.5, 25°C | Secale cereale | |
| 1.19 | - |
DIBOA-glucoside | pH 5.5, 25°C | Secale cereale | |
| 2 | - |
2-hydroxy-1,4-benzoxazin-3-one-beta-D-glucoside | pH 5.5, 25°C | Secale cereale | |
| 3.17 | - |
4-nitrophenyl beta-D-xyloside | pH 5.5, 25°C | Secale cereale | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 0.2 mM, complete inhibition | Secale cereale | |
| Cu2+ | 0.5 mM, strong inhibition | Secale cereale | |
| Fe2+ | 0.5 mM, inhibits activity | Secale cereale | |
| Mg2+ | 0.5 mM, inhibits activity | Secale cereale | |
| Mn2+ | 0.5 mM, inhibits activity | Secale cereale | |
| Zn2+ | 0.5 mM, inhibits activity | Secale cereale | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
SDS-PAGE, monomer | Secale cereale |
| 300000 | - |
native-PAGE, oligomer | Secale cereale |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Secale cereale | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using isoelectric precipitation, anion exchange chromatography, and gel filtration | Secale cereale |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Secale cereale | - |
| shoot | isolated from 48-h-old rye shoots | Secale cereale | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-1,4-benzoxazin-3-one-beta-D-glucoside + H2O | - |
Secale cereale | 2-hydroxy-1,4-benzoxazin-3-one + beta-D-glucose | - |
? | |
| 2-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O | - |
Secale cereale | 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-fucoside + H2O | - |
Secale cereale | 4-nitrophenol + beta-D-fucose | - |
? | |
| 4-nitrophenyl beta-D-glucoside + H2O | - |
Secale cereale | 4-nitrophenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-xyloside + H2O | - |
Secale cereale | 4-nitrophenol + beta-D-xylose | - |
? | |
| DIBOA-glucoside + H2O | - |
Secale cereale | DIBOA + beta-D-glucose | - |
? | |
| DIMBOA-glucoside + H2O | - |
Secale cereale | DIMBOA + beta-D-glucose | - |
? | |
| esculin + H2O | - |
Secale cereale | esculetin + beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | native-PAGE | Secale cereale |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glucosidase | - |
Secale cereale |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
optimal reaction at 25-30°C | Secale cereale |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Secale cereale |
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