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1,1,1-kestopentaose + H2O
?
1,1,1-kestopentaose + H2O
D-fructose + ?
1,1-kestotetraose + H2O
?
1-kestose + H2O
beta-D-fructose + sucrose
1-kestose + H2O
D-fructose + sucrose
from Vernonia herbacea and Vernonia discolor, but levans from Gomphrena macrocephala and Phleum pratense are poorly hydrolyzed, hydrolytic cleavage of terminal fructosyl residues off inulin, sucrose is not hydrolyzed
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-
?
1G-kestotetraose + H2O
?
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96% of the activity with 6G,1-kestotetraose
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?
6G,1-kestotetraose + H2O
?
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-
-
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?
6G,6-kestotetraose + H2O
?
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4% of the activity with 6G,1-kestotetraose
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?
6G-kestotetraose + H2O
?
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?
6G-kestotriose + H2O
?
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5% of the activity with 6G,1-kestotetraose
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?
fructan + H2O
D-fructose + ?
-
Agave tequilana fructans
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?
fructofuranosylnystose + H2O
?
inulin + H2O
D-fructose + ?
inulin + H2O
fructose + ?
inulin + H2O
fructose + fructo-oligosaccharide + fructo-polysaccharide
sucrose + H2O
D-fructose + D-glucose
wheat graminan + H2O
?
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?
additional information
?
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1,1,1-kestopentaose + H2O
?
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-
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?
1,1,1-kestopentaose + H2O
?
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?
1,1,1-kestopentaose + H2O
D-fructose + ?
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?
1,1,1-kestopentaose + H2O
D-fructose + ?
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1.2 mM in 50 mM Na-acetate buffer, pH 5.2, 0.02% Na-azide, 60 min, 30°C
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?
1,1-kestotetraose + H2O
?
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?
1,1-kestotetraose + H2O
?
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85% of the activity with 6G,1-kestotetraose
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?
1,1-kestotetraose + H2O
?
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?
1,1-kestotetraose + H2O
?
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?
1,1-nystose + H2O
?
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-
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?
1,1-nystose + H2O
?
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72% of the activity with 1-kestose, isoenzyme 1-FEH w1
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?
1,1-nystose + H2O
?
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83% of the activity with 1-kestose, isoenzyme 1-FEH w2
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?
1-kestose + H2O
?
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?
1-kestose + H2O
?
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?
1-kestose + H2O
beta-D-fructose + sucrose
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?
1-kestose + H2O
beta-D-fructose + sucrose
best substrate
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?
1-kestotriose + H2O
?
1-FEHa is a fructan exohydrolase with predominant activity towards beta-(2-1) linkages
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?
1-kestotriose + H2O
?
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88% of the activity with 6G,1-kestotetraose
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?
1-kestotriose + H2O
?
58% of the activity with 1,1-kestotetraose
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?
1-kestotriose + H2O
?
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?
6-kestose + H2O
?
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0.7% of the activity with 1-kestose, isoenzyme 1-FEH w1
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?
6-kestose + H2O
?
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1% of the activity with 1-kestose, isoenzyme 1-FEH w2
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?
6-kestotriose + H2O
?
lower activity compared to 1-kestotriose
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?
6-kestotriose + H2O
?
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?
fructofuranosylnystose + H2O
?
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?
fructofuranosylnystose + H2O
?
best substrate
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?
fructofuranosylnystose + H2O
?
high activity
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?
inulin + H2O
?
best substrate
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?
inulin + H2O
?
high activity
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?
inulin + H2O
?
24 of the activity with 1,1-kestotetraose
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?
inulin + H2O
?
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22% of the activity with 1-kestose, isoenzyme 1-FEH w2
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?
inulin + H2O
?
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39% of the activity with 1-kestose, isoenzyme 1-FEH w1
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?
inulin + H2O
D-fructose + ?
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?
inulin + H2O
D-fructose + ?
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?
inulin + H2O
D-fructose + ?
5% inulin from Vernonia herbacea and Vernonia discolor, but levans from Gomphrena macrocephala and Phleum pratense are poorly hydrolyzed, hydrolytic cleavage of terminal fructosyl residues off inulin, sucrose is not hydrolyzed
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?
inulin + H2O
fructose + ?
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?
inulin + H2O
fructose + ?
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multichain mechanism
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?
inulin + H2O
fructose + fructo-oligosaccharide + fructo-polysaccharide
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5% inulin from chicory, 30 degrees Celsius
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?
inulin + H2O
fructose + fructo-oligosaccharide + fructo-polysaccharide
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5% inulin from Helianthus tuberosus, McIlvaine buffer, pH 4.5, 30°C, 4 h
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?
levan + H2O
?
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3% of the activity with 1-kestose, isoenzyme 1-FEH w2
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?
levan + H2O
?
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4% of the activity with 1-kestose, isoenzyme 1-FEH w1
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?
neokestose + H2O
?
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3% of the activity with 1-kestose, isoenzyme 1-FEH w2
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?
neokestose + H2O
?
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4% of the activity with 1-kestose, isoenzyme 1-FEH w1
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?
nystose + H2O
?
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?
nystose + H2O
?
best substrate
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?
nystose + H2O
?
high activity
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?
sucrose + H2O
D-fructose + D-glucose
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?
sucrose + H2O
D-fructose + D-glucose
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3% of the activity with 6G,1-kestotetraose
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?
additional information
?
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Agave tequilana fructans are a polydisperse mixture of long degree of polymerization (DP) polymers and fructooligosaccharides (FOSs) with both b(2-1) and b(2-6) linkages. This mixture also contains highly branched agavins and graminans, and agavins are the most abundant of all. Agave tequilana fructans exhibit fluctuation of their total reducing sugar content and DP according to the plant age
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?
additional information
?
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hydrolysis of terminal, non-reducing 2,1-linked beta-D-fructofuranose residues in fructans
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?
additional information
?
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Bp1-FEHa is a fructan exohydrolase with predominant activity towards beta-2,1-linkages
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?
additional information
?
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Bp1-FEHa is a fructan exohydrolase with predominant activity towards beta-2,1-linkages
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?
additional information
?
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the enzyme is involved in fructan breakdown
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?
additional information
?
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isoform 1-FEH II is expressed when young chicory plants are defoliated, suggesting that this enzyme can be induced at any developmental stage when large energy supplies are necessary
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?
additional information
?
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the enzyme is involved in fructan degradation
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?
additional information
?
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the enzyme hydrolyzes the beta(2-1) linkage in inulin, 1-kestose and 1,1-nystose at rates at least 5fold faster than the beta(2-6) linkages in levan oligosaccharides and levanbiose
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?
additional information
?
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hydrolytic cleavage of terminal fructosyl residues off inulin
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?
additional information
?
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hydrolysis of Jerusalem artichoke tuber by immobilized isozyme Ci1-FEH IIa and analysis of carbohydrate profile. The fructan in the tuber is rapidly degraded to fructose by immobilized recombinant Ci1-FEH IIa. Substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH IIa has much higher activity than all other investigated FEH isozymes from Cichorium intybus and Helianthus tuberosus
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?
additional information
?
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hydrolysis of Jerusalem artichoke tuber by immobilized isozyme Ci1-FEH IIa and analysis of carbohydrate profile. The fructan in the tuber is rapidly degraded to fructose by immobilized recombinant Ci1-FEH IIa. Substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH IIa has much higher activity than all other investigated FEH isozymes from Cichorium intybus and Helianthus tuberosus
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?
additional information
?
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hydrolysis of Jerusalem artichoke tuber by immobilized isozyme Ci1-FEH IIa and analysis of carbohydrate profile. The fructan in the tuber is rapidly degraded to fructose by immobilized recombinant Ci1-FEH IIa. Substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH IIa has much higher activity than all other investigated FEH isozymes from Cichorium intybus and Helianthus tuberosus
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?
additional information
?
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hydrolysis of Jerusalem artichoke tuber by immobilized isozyme Ci1-FEH IIa and analysis of carbohydrate profile. The fructan in the tuber is rapidly degraded to fructose by immobilized recombinant Ci1-FEH IIa. Substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH IIa has much higher activity than all other investigated FEH isozymes from Cichorium intybus and Helianthus tuberosus
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?
additional information
?
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substrate specificity, overview. No activity with sucrose and levan
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?
additional information
?
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substrate specificity, overview. No activity with sucrose and levan
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?
additional information
?
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substrate specificity, overview. No activity with sucrose and levan
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?
additional information
?
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substrate specificity, overview. No activity with sucrose and levan
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?
additional information
?
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substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH I shows quite low activity compared to other FEH isozymes analyzed from Cichorium intybus and Helianthus
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?
additional information
?
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substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH I shows quite low activity compared to other FEH isozymes analyzed from Cichorium intybus and Helianthus
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?
additional information
?
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substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH I shows quite low activity compared to other FEH isozymes analyzed from Cichorium intybus and Helianthus
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?
additional information
?
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substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH I shows quite low activity compared to other FEH isozymes analyzed from Cichorium intybus and Helianthus
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?
additional information
?
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the enzyme hydrolyzes terminal beta-(2-1)-fructosylfructose-linkages in linear and branched fructan oligomers. beta-(2-6)-linkages are hardly hydrolyzed
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?
additional information
?
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high expression level in conditions of active fructan synthesis, low expression level when fructan contents are low. The enzyme might play a role as a beta-(2,1) trimming enzyme acting during fructan synthesis in concert with fructan synthesis enzymes
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?
additional information
?
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high expression level in conditions of active fructan synthesis, low expression level when fructan contents are low. The enzyme might play a role as a beta-(2,1) trimming enzyme acting during fructan synthesis in concert with fructan synthesis enzymes
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?
additional information
?
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low activity against 6-kestotriose and levan. No hydrolase activity can be detected towards sucrose
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?
additional information
?
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low activity against 6-kestotriose and levan. No hydrolase activity can be detected towards sucrose
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?
additional information
?
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isoenzyme 1-FEH w2 might play a role as a beta-(2,1)-trimmer throughout the period of active graminan biosynthesis
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?
additional information
?
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isozyme Wfh-sm3m hydrolyzes beta-(2,6)-linkages of fructans (6-kestotriose) more efficiently than beta-(2,1)-linkages of fructans (1-kestotriose and 1,1-kestotetraose). On the other hand, Wfh-sm3m cannot hydrolyze long-chain fructans such as inulin and bacterial levan
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?
additional information
?
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wheat graminan degradation by Wfh-sm3 recombinant protein Wfh-sm3m. The recombinant protein Wfh-sm3m preferentially degrades 6-kestotriose and possesses minor hydrolase activity to 1-kestotriose and 1,1-kestotetraose, poor activity with sucrose, and no activity with inulin and levan
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?
additional information
?
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hydrolytic cleavage of terminal fructosyl residues off inulin
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?
additional information
?
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a set of fructan 1-exohydrolase proteins acts in two different ways during fructan mobilization: (1) by hydrolyzing fructo-oligosaccharides and -polysaccharides in sprouting plants (naturally or induced) for carbon supply and (2) by hydrolyzing preferably fructo-polysaccharides under low temperature to maintain the oligosaccharide pool for plant cold acclimation
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?