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Literature summary for 3.2.1.153 extracted from

  • Lothier, J.; Lasseur, B.; Le Roy, K.; Van Laere, A.; Prudhomme, M.P.; Barre, P.; Van den Ende, W.; Morvan-Bertrand, A.
    Cloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilization (2007), J. Exp. Bot., 58, 1969-1983.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Lolium perenne

Inhibitors

Inhibitors Comment Organism Structure
sucrose
-
Lolium perenne

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lolium perenne high expression level in conditions of active fructan synthesis, low expression level when fructan contents are low. The enzyme might play a role as a beta-(2,1) trimming enzyme acting during fructan synthesis in concert with fructan synthesis enzymes ?
-
?

Organism

Organism UniProt Comment Textmining
Lolium perenne Q64GB3 L. var. Bravo
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1-kestotetraose + H2O
-
Lolium perenne ?
-
?
1-kestotriose + H2O 58% of the activity with 1,1-kestotetraose Lolium perenne ?
-
?
inulin + H2O 24 of the activity with 1,1-kestotetraose Lolium perenne ?
-
?
additional information high expression level in conditions of active fructan synthesis, low expression level when fructan contents are low. The enzyme might play a role as a beta-(2,1) trimming enzyme acting during fructan synthesis in concert with fructan synthesis enzymes Lolium perenne ?
-
?
additional information low activity against 6-kestotriose and levan. No hydrolase activity can be detected towards sucrose Lolium perenne ?
-
?

Synonyms

Synonyms Comment Organism
1-FEH
-
Lolium perenne
Lp1-FEHa
-
Lolium perenne

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Lolium perenne

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 30°C: about 70% of maximal activity, 50°C: about 30% of maximal activity Lolium perenne

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Lolium perenne

pH Range

pH Minimum pH Maximum Comment Organism
4.3 6.3 pH 4.3: about 60% of maximal activity, pH 6.3: about 50% of maximal activity Lolium perenne

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8
-
sucrose
-
Lolium perenne

pI Value

Organism Comment pI Value Maximum pI Value
Lolium perenne
-
-
5.2