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Literature summary for 3.2.1.153 extracted from

  • Portes, M.T.; Figueiredo-Ribeiro, R.d.e..C.; de Carvalho, M.A.
    Low temperature and defoliation affect fructan-metabolizing enzymes in different regions of the rhizophores of Vernonia herbacea (2008), J. Plant Physiol., 165, 1572-1581.
    View publication on PubMed
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Vernonia herbacea hydrolytic cleavage of terminal fructosyl residues off inulin ?
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 ?

Organism

Organism UniProt Comment Textmining
Vernonia herbacea
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 18 months old plants, natural outdoor condition (control), excised natural conditions, excised and intact plants at 5°C for 21 days
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Purification (Commentary)

Purification (Comment) Organism
precipitated, resuspended, desalted, centrifuged Vernonia herbacea

Source Tissue

Source Tissue Comment Organism Textmining
rhizophore
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 Vernonia herbacea
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 highest activities in proximal rhizophores, increased in low temperature and excised plants, and low temperature + excised plants, overall highest activities in low temperature + excised plant rhizophores, overall high fructan content in all treatments, higher fructo-oligosaccharide/fructo-polysaccharide ratios in low temperature treatments, chain size lowest in distal region Vernonia herbacea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O 5% inulin from Helianthus tuberosus, McIlvaine buffer, pH 4.5, 30°C, 4 h Vernonia herbacea fructose + fructo-oligosaccharide + fructo-polysaccharide
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 ?
additional information hydrolytic cleavage of terminal fructosyl residues off inulin Vernonia herbacea ?
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 ?

Synonyms

Synonyms Comment Organism
1-FEH
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 Vernonia herbacea
1-fructan exohydrolase
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 Vernonia herbacea