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3.2.1.153: fructan beta-(2,1)-fructosidase

This is an abbreviated version!
For detailed information about fructan beta-(2,1)-fructosidase, go to the full flat file.

Word Map on EC 3.2.1.153

Reaction

1,1,1,1-kestohexaose
+
H2O
=
1,1,1-kestopentaose
 +
beta-D-fructofuranose

Synonyms

1-FEH, 1-FEH I, 1-FEH II, 1-FEH IIa, 1-FEH w1, 1-FEH w2, 1-FEH2a, 1-FEH2b, 1-FEHa, 1-FEHI, 1-FEHw1, 1-fructan exohydrolase, 1-fructoexohydrolase, beta-(2-1) fructan exohydrolase, beta-(2-1)-D-fructan fructohydrolase, beta-(2-1)-linkage specific FEH, beta-(2-1)-linkage specific fructan-beta-fructosidase, beta-(2-1)-linkage-specific fructan-beta-fructosidase, Ci1-FEH I, Ci1-FEH IIa, Ci1-FEH IIb, FEH, fructan 1-exohydrolase, fructan 1-exohydrolase I, fructan 1-exohydrolase IIa, fructan 1-exohydrolase IIb, fructan 1-exohydrolase w1, fructan 1-exohydrolase w2, fructan exohydrolase, fructanexohydrolase, Lp1-FEH, Lp1-FEHa, Wfh-sm3m

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.153 fructan beta-(2,1)-fructosidase

Cloned

Cloned on EC 3.2.1.153 - fructan beta-(2,1)-fructosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
48 h in Nicotiana benthamiana leaves infiltrated by Agrobacterium tumefaciens C58C1 with entry vector pDONR(TM)Zeo and destination vector pMDC32
-
enzyme expression analysis of isozymes under different water conditions, overview
-
expressed in Pichia pastoris
expression in Pichia pastoris
fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb
-
gene 1-FEH1, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview
gene 1-FEH2a, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview
gene 1-FEH2b, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview
gene 1-FEHa, quantitative RT-PCR expression analysis
gene FEH-1, real-time PCR quantitative expression analysis
gene FEH-I, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33
gene FEH-IIa, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33
gene FEH-IIb, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33
gene Wfh-sm3, recombinant expression in Pichia pastoris
-
heterologous expression in Pichia pastoris strain X-33 after culturing of plasmids in Escherichia coli, expression vector pPICZalphaA
heterologous expression in Pichia pastoris, pPICZalphaA vector
-
isoenzyme 1-FEH w1
-
isoenzyme 1-FEH w2
-
quantitative real time PCR analysis of the FEH mRNAs (including FEHI and FEHII) in cold stored roots of three chicory cultivars, overview. Variable levels of FEHII and FEHI expression in different phenotypes of the chicory cultivars
single copy gene Bp1-FEHa, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression in Pichia pastoris