3.2.1.142: limit dextrinase

This is an abbreviated version!
For detailed information about limit dextrinase, go to the full flat file.

Reaction

Synonyms

Ask, GH13 12-14, HvLD99, limit dextrinase, Pul3YH5, PULI, pullulanase, pullulanase type I, starch-debranching enzyme

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.142 limit dextrinase

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Results	in table
3	Activating Compound
8	AA Sequence
10	Application
1	CAS Registry Number
8	Cloned(Commentary)
4	Crystallization (Commentary)
2	Expression
6	General Information
33	Inhibitors
3	kcat/KM [mM/s]
22	KM Value [mM]
11	Metals/Ions
12	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
41	Organism
11	pH Optimum
2	pH Stability
1	pI Value
11	Protein Variants
7	Purification (Commentary)
1	Reaction
1	Reaction Type
39	Reference
12	Source Tissue
6	Specific Activity [micromol/min/mg]
70	Substrates and Products (Substrate)
9	Subunits
14	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
8	Temperature Stability [°C]
7	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 3.2.1.142 - limit dextrinase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

30 - 60

Anoxybacillus sp.

EPZ37738

20 min, stable

737518

30 - 70

Eragrostis tef

-

enzyme activity during kilning at different temperatures, first-order rate constants, overview. the enzyme is stable up to 50°C for 40 h, increases in activity at 30°C for 30 h, and loses activity at 70°C

731837

50

Exiguobacterium acetylicum

I3WU34

pH 5-8, 10 min, more than 70% of maximum activity

738951

60

2 entries

65

Hordeum vulgare

-

there is a near total loss in enzyme activity in under 10 min at 65°C

746543

70

Hordeum vulgare

-

malted enzyme, strong decrease in activity within 1-2 days during mashing

731989

additional information

Hordeum vulgare

O48541

the level of limit dextrinase thermostability is inversely correlated with total limit dextrinase activity

709403

60

Anoxybacillus sp.

EPZ37738

9 h, 50% residual activity

737518

60

Sorghum bicolor

-

60% inactivation

208513