3.2.1.142: limit dextrinase

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For detailed information about limit dextrinase, go to the full flat file.

Reaction

Synonyms

Ask, GH13 12-14, HvLD99, limit dextrinase, Pul3YH5, PULI, pullulanase, pullulanase type I, starch-debranching enzyme

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.142 limit dextrinase

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Results	in table
3	Activating Compound
8	AA Sequence
10	Application
1	CAS Registry Number
8	Cloned(Commentary)
4	Crystallization (Commentary)
2	Expression
6	General Information
33	Inhibitors
3	kcat/KM [mM/s]
22	KM Value [mM]
11	Metals/Ions
12	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
41	Organism
11	pH Optimum
2	pH Stability
1	pI Value
11	Protein Variants
7	Purification (Commentary)
1	Reaction
1	Reaction Type
39	Reference
12	Source Tissue
6	Specific Activity [micromol/min/mg]
70	Substrates and Products (Substrate)
9	Subunits
14	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
8	Temperature Stability [°C]
7	Turnover Number [1/s]

Application

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Application on EC 3.2.1.142 - limit dextrinase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

analysis

3 entries

brewing

Hordeum vulgare

-

the enzyme bound to the limit dextrinase inhibitor is a limiting factor for complete starch digestion during brewing

746543

food industry

5 entries

synthesis

Hordeum vulgare

O48541

expression of the limit dextrinase encoding gene fragment without signal peptide, with the Saccharomyces cerevisiae alpha-factor secretion signal under control of the alcohol oxidase 1 promoter, in Pichia pastoris leads to highly active barley limit dextrinase secreted during high cell-density fermentation. Optimization of a fedbatch fermentation procedure enables efficient production in a 5-l bioreactor, yielding 34 mg homogenous enzyme with 84% recovery

710478

analysis

Hordeum vulgare

O48541

methods for a rapid and cost efficient assay of both beta-amylase and limit dextrinase thermostability

709403

analysis

Hordeum vulgare

-

assay for limit dextrinase activity using a chromogenic assay. The assay utilizes a small soluble chromogenic substrate which is hydrolyzed selectively by limit dextrinase in a coupled assay to release the chromophore 2-chloro-4-nitrophenol. The results obtained are comparable with the results obtained from a commercially available assay. The improved assay uses a soluble substrate, being suited for high-throughput screening

738436

analysis

Oryza sativa

-

substrate 4,6-O-benzylidene-2-chloro-4-nitrophenyl-beta-63-alpha-D-maltotriosyl-maltotrioside (BzCNPG3G3, Hexachrom) is not susceptible to transglycosylation and serves amiably as a routine quantitative assay tool with the potential to run kinetic assays due to the low pKa (about 5.5) of the chromogenic moiety. Substrate 4,6-O-benzylidene-4-methylumbelliferyl-beta-63-alpha-D-maltotriosyl-maltotrioside (BzMUG3G3, Hexafluor) is susceptible to transglycosylation. Both substrates are applicable to industrial standard assays

738748

food industry

Hordeum vulgare

O48541

the prediction of malt fermentability is achieved by both forward step-wise multi-linear regression and the partial least squares multivariate model development methods. Both methods produce similar identifications of the parameters predicting wort fermentability at similar levels of predictive power. Both models are substantially better at predicting fermentability than the traditional use of diastatic power on its own. Limit dextrinase thermostability is not a substantial predictor of fermentability, presumably due to its negative correlation with total limit dextrinase activity. The application of these insights in the malting and brewing industries is expected to result in substantial improvements in brewing consistency and enable more specific quality targets for barley breeder’s progeny selection cut-off limits to be more precisely defined

709403

food industry

Hordeum vulgare

-

limit dextrinase activity is of particular importance for the brewing industry as the branched dextrins produced after the action of alpha-amylase on starch are not fermentable and represent a loss of potential ethanol production

732393

food industry

Hordeum vulgare

-

limit dextrinase is a unique debranching enzyme involved in starch mobilization of barley grains during malting, and closely related to malt quality

731524

food industry

Eragrostis tef

-

the enzyme is important for malting, influence of kilning on the enzyme activity, process optimization overview

731837

food industry

Hordeum vulgare

-

the enzyme is used in brewing and malting, the enzyme is limited for starch degradation due to its thermolability during kilning at 70°C, overview

731989