3.2.1.142: limit dextrinase

This is an abbreviated version!
For detailed information about limit dextrinase, go to the full flat file.

Ask, GH13 12-14, HvLD99, limit dextrinase, Pul3YH5, PULI, pullulanase, pullulanase type I, starch-debranching enzyme

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Results	in table
3	Activating Compound
8	AA Sequence
10	Application
1	CAS Registry Number
8	Cloned(Commentary)
4	Crystallization (Commentary)
2	Expression
6	General Information
33	Inhibitors
3	kcat/KM [mM/s]
22	KM Value [mM]
11	Metals/Ions
12	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
41	Organism
11	pH Optimum
2	pH Stability
1	pI Value
11	Protein Variants
7	Purification (Commentary)
1	Reaction
1	Reaction Type
39	Reference
12	Source Tissue
6	Specific Activity [micromol/min/mg]
70	Substrates and Products (Substrate)
9	Subunits
14	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
8	Temperature Stability [°C]
7	Turnover Number [1/s]

Purification on EC 3.2.1.142 - limit dextrinase

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acetone precipitation, affinity chromatography

acetone precipitation, DEAE-cellulose, Sephadex G-150

continuous electrophoresis, starch-celite, Sephadex G-150

enzyme from malted barley 31.23fold by ammonium sulfate fractionation and column chromatography

from rice grain