3.2.1.142: limit dextrinase
This is an abbreviated version!
For detailed information about limit dextrinase, go to the full flat file.
Word Map on EC 3.2.1.142
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3.2.1.142
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barley
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malt
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pullulan
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amylopectin
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isoamylase
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diastatic
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3.2.1.41
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pullulanases
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analysis
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starch-branching
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beta-limit
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food industry
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beta-amylase
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synthesis
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brewing
- 3.2.1.142
- barley
- malt
- pullulan
- amylopectin
- isoamylase
-
diastatic
-
3.2.1.41
- pullulanases
- analysis
-
starch-branching
-
beta-limit
- food industry
- beta-amylase
- synthesis
- brewing
Reaction
Synonyms
Ask, GH13 12-14, HvLD99, limit dextrinase, Pul3YH5, PULI, pullulanase, pullulanase type I, starch-debranching enzyme
ECTree
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KM Value
KM Value on EC 3.2.1.142 - limit dextrinase
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0.192
4,6-O-benzylidene-2-chloro-4-nitrophenyl-beta-63-alpha-D-maltotriosyl-maltotrioside
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pH.0, 40°C
0.052
4,6-O-benzylidene-4-methylumbelliferyl-beta-63-alpha-D-maltotriosyl-maltotrioside
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pH.0, 40°C
0.135
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pH 5.3, 45°C, barley malt extract
0.154
2-chloro-4-nitrophenyl maltotriosyl-alpha(1->6)-maltotriosyl-beta-D-glucose
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pH 5.3, 45°C, recombinant enzyme
additional information
Km value of wild-type 6.9 mg/ml, mutant M440G 4.4 mg/ml, respectively, 37°C, pH not specified in the publication
additional information
amylopectin
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Km value of wild-type 6.9 mg/ml, mutant M440G 4.4 mg/ml, respectively, 37°C, pH not specified in the publication
additional information
additional information
classical Michaelis-Menten kinetics results in Km of 0.081 mg/ml, fitting the uncompetitive substrate inhibition Michaelis-Menten equation results in Km 0.16 mg/ml, pH 5.5, 40°C
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additional information
additional information
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classical Michaelis-Menten kinetics results in Km of 0.081 mg/ml, fitting the uncompetitive substrate inhibition Michaelis-Menten equation results in Km 0.16 mg/ml, pH 5.5, 40°C
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additional information
pullulan
Km value 0.12 mg/ml, pH 6.0, 50°C
additional information
pullulan
Km value of wild-type 0.16 mg/ml, mutant M440G 0.15 mg/ml, respectively, 37°C, pH not specified in the publication
additional information
pullulan
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Km value of wild-type 0.16 mg/ml, mutant M440G 0.15 mg/ml, respectively, 37°C, pH not specified in the publication
additional information
soluble starch
Km value 0.69 mg/ml,pH 6.0, 50°C
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