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Literature summary for 3.2.1.142 extracted from
- The limit dextrinase from malted sorghum (Sorghum vulgare) (1976), Carbohydr. Res., 50, 75-85.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
pullulan | - |
Sorghum bicolor |  |
| 1.5 | - |
amylopectin beta-limit dextrin | - |
Sorghum bicolor | |
| 1.5 | - |
6-alpha-maltotriosylmaltotetraose | - |
Sorghum bicolor | |
| 26 | - |
glycogen beta-limit dextrin | - |
Sorghum bicolor |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
gel filtration | Sorghum bicolor |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polysaccharide + H2O | Sorghum bicolor | - |
oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sorghum bicolor | - |
malted sorghum | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| continuous electrophoresis, starch-celite, Sephadex G-150 | Sorghum bicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.08 | - |
- |
Sorghum bicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-alpha-maltotriosylmaltotetraose + H2O | - |
Sorghum bicolor | ? | - |
? | |
| amylopectin beta-limit dextrin + H2O | - |
Sorghum bicolor | maltotriose + maltose + maltooligosaccharides | - |
? | |
| glycogen beta-limit dextrin + H2O | - |
Sorghum bicolor | maltotriose + maltose + maltooligosaccharides | - |
? | |
| polysaccharide + H2O | - |
Sorghum bicolor | oligosaccharides | - |
? | |
| polysaccharide + H2O | oligosaccharides containing maltosyl or larger side chains are hydrolysed as well as amylopectin and amylopectin beta-limit dextrin, oligo- and poly-saccharides containing single D-glucosyl groups attached by an alpha-(1,6) linkage to the rest of the molecule are not hydrolysed, glycogen is not a substrate | Sorghum bicolor | oligosaccharides | - |
? | |
| pullulan + H2O | - |
Sorghum bicolor | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
60% inactivation | Sorghum bicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.4 | pullulan as substrate | Sorghum bicolor |
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Release 2023.1 (January 2023)
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