3.1.13.1: exoribonuclease II
This is an abbreviated version!
For detailed information about exoribonuclease II, go to the full flat file.
Word Map on EC 3.1.13.1
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3.1.13.1
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dnase
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nucleic
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pancreatic
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strand
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single-stranded
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endoribonuclease
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viruses
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polynucleotide
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endonuclease
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polyadenylation
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duplex
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polya
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ribonucleoproteins
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transcriptase
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rna-binding
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circrnas
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exosome
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phosphorylase
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chemokine
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dsrnas
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tata
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virion
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rpa
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nucleases
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stem-loops
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actinomycin
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helicase
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endonucleolytic
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dicer
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phosphodiester
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rna-dependent
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pronase
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intermedius
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oligoadenylate
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riboprobes
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rna-protein
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nucleolar
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ribozyme
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3'-terminal
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angiogenin
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argonaute
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encapsidation
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rna-induced
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pre-rrnas
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rna-dna
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antitoxin
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mip-1alpha
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snrnp
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medicine
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drosha
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degradation
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analysis
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norovirus
- 3.1.13.1
- dnase
- nucleic
- pancreatic
- strand
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single-stranded
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endoribonuclease
- viruses
- polynucleotide
- endonuclease
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polyadenylation
- duplex
- polya
- ribonucleoproteins
- transcriptase
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rna-binding
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circrnas
-
exosome
- phosphorylase
- chemokine
- dsrnas
- tata
- virion
- rpa
- nucleases
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stem-loops
- actinomycin
- helicase
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endonucleolytic
- dicer
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phosphodiester
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rna-dependent
- pronase
- intermedius
- oligoadenylate
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riboprobes
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rna-protein
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nucleolar
-
ribozyme
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3'-terminal
-
angiogenin
-
argonaute
-
encapsidation
-
rna-induced
- pre-rrnas
- rna-dna
- antitoxin
-
mip-1alpha
-
snrnp
- medicine
- drosha
- degradation
- analysis
- norovirus
Reaction
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates =
Synonyms
3'-5'exoribonuclease, 3’-5’exoribonuclease, 5'->3' exoribonuclease 2, AB205_0003320, Dis, Dis3, EC 3.1.4.20, exonuclease ISG20, More, PfRNase II, RC-RNase 2, ribonuclease 2, ribonuclease II, ribonuclease Q, Ribonuclease R, RNase, RNase 2, RNase A, RNase II, RNase R, RNase-2, RNaseR, Rnb, RNR, RNR1, Rrp44, XRN2
ECTree
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Inhibitors
Inhibitors on EC 3.1.13.1 - exoribonuclease II
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DNA oligonucleotide with strong duplex structure, 3' and 5' single strand
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DNA oligonucleotide with weak duplex structure, 3' and 5' single strand
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DNA stem-loop structure
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free 3'- and 5'-arms needed for potent inhibition, weaker stem-loops are better inhibitors than their counterpart with a strong duplex
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NaCl
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a salt concentration of 0.05 M inhibits 50% of the activity, salt optimum: 0.01 M
oligonucleotides
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competitive inhibition, fragments bind without being destroyed; small to medium sized
RNA stem-loop structure
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built by repetitive extragenic palindromic sequences; the lower the stability of the RNA-stem, the faster the degradation
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sucrose
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slight inhibition, in 1% and 5% sucrose 5% and 20% of the activity inhibited
thymidine nucleotides
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potent inhibitor, pTp at a concentration of 0.00004 M inhibits degradation of poly(A) by 60%, pTpT at a concentration of 0.0005 M inhibits degradation of poly(A) by 30%
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monovalent or divalent cations
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10 mM of Mg2+ reduce activity 2fold, activity barely detectable at 20 mM Mg2+; high concentrations
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monovalent or divalent cations
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high concentrations; Mg2+-concentrations above 2 mM
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RNA with a terminal 3'-phosphate group
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on the potential substrate molecule
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competitive inhibition, potent inhibitors have a poly(dC)-chain length of 23-29
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in 0.5 and 0.05 M urea 58% and 11% of the activity inhibited, complete inhibition at 1 M urea
Urea
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loss of 40% of activity in presence of 1.6 M, 60% in presence of 2.4 M and 75% in presence of 3.2 M urea
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RNase II activity is blocked by the presence of double-stranded structures on the RNA molecule
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additional information
acetylation at enzyme residue Lys501 affects binding of the substrate and decreases the catalytic activity of RNase II
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additional information
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acetylation at enzyme residue Lys501 affects binding of the substrate and decreases the catalytic activity of RNase II
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