Inhibitors | Comment | Organism | Structure |
---|---|---|---|
monovalent or divalent cations | 10 mM of Mg2+ reduce activity 2fold, activity barely detectable at 20 mM Mg2+; high concentrations | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Escherichia coli | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | necessary for activity | Escherichia coli | |
Mg2+ | optimum: 2 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
precursor tRNA + H2O | Escherichia coli | - |
mature tRNA + 5'-phosphomononucleotides | - |
? | |
ss RNA + H2O | Escherichia coli | - |
5'-phosphomononucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification, between 50-100fold | Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, stable for at least 1 month without loss of activity | Escherichia coli |
0°C, half-life of 5-7 days | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
precursor tRNA + H2O | - |
Escherichia coli | mature tRNA + 5'-phosphomononucleotides | - |
? | |
ss RNA + H2O | - |
Escherichia coli | 5'-phosphomononucleotides | - |
? | |
tRNA + H2O | RNase BN | Escherichia coli | ? | - |
? |