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Literature summary for 3.1.13.1 extracted from
- Purification and properties of ribonuclease II from Escherichia coli (1964), J. Biol. Chem., 239, 3716-3726.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cadaverine | can replace missing Mg2+ to a small extent | Escherichia coli |  |
| putrescine | can replace missing Mg2+ to a small extent | Escherichia coli | |
| spermidine | can replace missing Mg2+ to a small extent | Escherichia coli | |
| spermine | can replace missing Mg2+ to a small extent | Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | competitive inhibitor | Escherichia coli | |
| EDTA | - |
Escherichia coli | |
| monovalent or divalent cations | high concentrations | Escherichia coli | |
| oligonucleotides | competitive inhibition, fragments bind without being destroyed; small to medium sized | Escherichia coli | |
| sucrose | slight inhibition, in 1% and 5% sucrose 5% and 20% of the activity inhibited | Escherichia coli | |
| Urea | in 0.5 and 0.05 M urea 58% and 11% of the activity inhibited, complete inhibition at 1 M urea | Escherichia coli | |
| Zn2+ | competitive inhibitor | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000075 | - |
Poly(U) | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Escherichia coli | 5829 | - |
| ribosome | - |
Escherichia coli | 5840 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | necessary for activity | Escherichia coli | |
| Mg2+ | necessary for activity | Escherichia coli | |
| Mn2+ | necessary for activity | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ss RNA + H2O | Escherichia coli | - |
5'-phosphomononucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 30fold | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates | can act as a endonuclease too | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, lyophilized purified enzyme, addition of 25 mg of bovine serum mercaptalbumin, stable for at least 2 months | Escherichia coli |
| deep frozen, lyophilized crude enzyme, several months, stable | Escherichia coli |
| frozen, crude enzyme, 1 month, stable | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(U) + H2O | - |
Escherichia coli | 5'-UMP + oligonucleotide | - |
? | |
| ss RNA + H2O | - |
Escherichia coli | 5'-phosphomononucleotides | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 11% of the optimal activity observed at pH 6.0, 55% at pH 9.0 | Escherichia coli |
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