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Literature summary for 3.1.13.1 extracted from

  • Coburn, G.A.; Mackie, G.A.
    Overexpression, purification, and properties of Escherichia coli ribonuclease II (1996), J. Biol. Chem., 271, 1048-1053.
    View publication on PubMed
Search for more literature for 3.1.13.1

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Escherichia coli
ds plasmid DNA
-
 Escherichia coli
ss DNA oligonucleotides
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ss RNA + H2O Escherichia coli
-
 5'-phosphomononucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates acts in a processive manner Escherichia coli
a

Renatured (Commentary)

Renatured (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
68.3
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ss RNA + H2O
-
 Escherichia coli 5'-phosphomononucleotides
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 for 5 min, in absence of substrate, less than 1% of activity remains Escherichia coli
37
-
 in presence of substrate, product or deoxy-oligonucleotides (dC)27: active up to 60 min Escherichia coli