3.1.13.1: exoribonuclease II
This is an abbreviated version!
For detailed information about exoribonuclease II, go to the full flat file.
Word Map on EC 3.1.13.1
-
3.1.13.1
-
dnase
-
nucleic
-
pancreatic
-
strand
-
single-stranded
-
endoribonuclease
-
viruses
-
polynucleotide
-
endonuclease
-
polyadenylation
-
duplex
-
polya
-
ribonucleoproteins
-
transcriptase
-
rna-binding
-
circrnas
-
exosome
-
phosphorylase
-
chemokine
-
dsrnas
-
tata
-
virion
-
rpa
-
nucleases
-
stem-loops
-
actinomycin
-
helicase
-
endonucleolytic
-
dicer
-
phosphodiester
-
rna-dependent
-
pronase
-
intermedius
-
oligoadenylate
-
riboprobes
-
rna-protein
-
nucleolar
-
ribozyme
-
3'-terminal
-
angiogenin
-
argonaute
-
encapsidation
-
rna-induced
-
pre-rrnas
-
rna-dna
-
antitoxin
-
mip-1alpha
-
snrnp
-
medicine
-
drosha
-
degradation
-
analysis
-
norovirus
- 3.1.13.1
- dnase
- nucleic
- pancreatic
- strand
-
single-stranded
-
endoribonuclease
- viruses
- polynucleotide
- endonuclease
-
polyadenylation
- duplex
- polya
- ribonucleoproteins
- transcriptase
-
rna-binding
-
circrnas
-
exosome
- phosphorylase
- chemokine
- dsrnas
- tata
- virion
- rpa
- nucleases
-
stem-loops
- actinomycin
- helicase
-
endonucleolytic
- dicer
-
phosphodiester
-
rna-dependent
- pronase
- intermedius
- oligoadenylate
-
riboprobes
-
rna-protein
-
nucleolar
-
ribozyme
-
3'-terminal
-
angiogenin
-
argonaute
-
encapsidation
-
rna-induced
- pre-rrnas
- rna-dna
- antitoxin
-
mip-1alpha
-
snrnp
- medicine
- drosha
- degradation
- analysis
- norovirus
Reaction
Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates =
Synonyms
3'-5'exoribonuclease, 3’-5’exoribonuclease, 5'->3' exoribonuclease 2, AB205_0003320, Dis, Dis3, EC 3.1.4.20, exonuclease ISG20, More, PfRNase II, RC-RNase 2, ribonuclease 2, ribonuclease II, ribonuclease Q, Ribonuclease R, RNase, RNase 2, RNase A, RNase II, RNase R, RNase-2, RNaseR, Rnb, RNR, RNR1, Rrp44, XRN2
ECTree
Advanced search results
Metals Ions
Metals Ions on EC 3.1.13.1 - exoribonuclease II
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
K+
KCl
-
preferred concentration is 50 mM. Activity starts to decrease above 150 mM
Mg2+
Mn2+
Na+
-
increases activity against T4 mRNA, can substitute for K+ in activation against T4 mRNA and RNA
NaCl
-
enzyme is active in NaCl, but the cleavage efficiency is 10times lower when compared to its activity in KCl
NH4+
Zn2+
-
induces a folding reaction resulting in protein preparations with 50-60% alpha-helical content and 10-20% beta-sheet structure, critical concentration for refolding bigger or equal to 0.1 mM
K+
-
maximum concentration for activation: 0.15 mM at pH 7.4 and 8.0
Mg2+
Asp209, but not Asp207, contacting Mg2+ in addition to Asp201, Asp210, and the phosphate group of nt 13
Mg2+
-
is important for substrate binding of RNase R in the channel as well as for catalysis. Asp278 is predicted to coordinate a Mg2+ at the catalytic center of RNase R
Mg2+
required, no degradation of the RNA occurs in the absence of Mg2+
Mn2+
-
optimum: 0.3 mM, stimulation about two-thirds the rate with Mg2+