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1.6.1.2: NAD(P)+ transhydrogenase (Re/Si-specific)

This is an abbreviated version!
For detailed information about NAD(P)+ transhydrogenase (Re/Si-specific), go to the full flat file.

Word Map on EC 1.6.1.2

Reaction

NADPH
+
NAD+
=
NADP+
 +
NADH

Synonyms

dII, dIII, energy-linked transhydrogenase, energy-linked, mitochondrial transhydrogenase, H+-thase, mitochondrial transhydrogenase, NAD transhydrogenase, NAD(P) transhydrogenase, NADH transhydrogenase, NADH-NADP-transhydrogenase, NADPH transhydrogenase, NADPH-NAD oxidoreductase, NADPH-NAD transhydrogenase, NADPH:NAD+ transhydrogenase, nicotinamide adenine dinucleotide (phosphate) transhydrogenase, nicotinamide nucleotide transhydrogenase, NNT, PNT, PntAB, proton-translocating nicotinamide nucleotide transhydrogenase, proton-translocating transhydrogenase, pyridine nucleotide transferase, pyridine nucleotide transhydrogenase, reversible mitochondrial membrane-associated transhydrogenase, transhydrogenase, transhydrogenase, nicotinamide adenine dinucleotide (phosphate)

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.1 With NAD+ or NADP+ as acceptor
                1.6.1.2 NAD(P)+ transhydrogenase (Re/Si-specific)

Inhibitors

Inhibitors on EC 1.6.1.2 - NAD(P)+ transhydrogenase (Re/Si-specific)

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2',5'-ADP
-
bacteriorhodopsin co-reconstituted enzyme, 36.8% inhibition of thio-NADP+ reduction by NADH in the dark, 34.4% in the light
2'-AMP
2,2'-dithiodipyridine
-
0.2 mM, 45% inhibition
2,2'-thiodiethanethiole
-
0.5 mM, 37% inhibition
2,4-dinitrophenyl-3'-dephospho-CoA
-
competitive vs. NAD+, non-competitive vs. NADPH
3'-5'-AMP
-
-
3'-AMP
-
-
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
34% residual activity at 1 mM
5'-adenosine diphosphate ribose
-
bacteriorhodopsin co-reconstituted enzyme, 29.6% inhibition of thio-NADP+ reduction by NADH in the dark, 13.2% in the light
5'-AMP
5'-[p-(fluorosulfonyl)benzoyl]-adenosine
-
structural analog of adenosine, 2 mM, almost complete inactivation after 25 min, acetylpyridine adenine dinucleotide, NADP+, 5'-AMP, 5'-ADP or a mixture of 2'-AMP and 3'-AMP protect from inactivation, NADPH accelerates the inhibition rate, inhibition rate constant increases 50fold by increasing the pH from 6.0 to 8.5
5,5'-dithiobis(2-nitrobenzoate)
acetyl-CoA
-
-
acetyl-dephospho-CoA
-
competitive vs. NAD(H)
acetylpyridine adenine dinucleotide
-
-
adenosine
ADP
-
-
Butane-2,3-dione
-
-
cardiolipin
-
noncompetitive vs. NAD+ and NADPH
D2O
-
-
Dansyl chloride
-
0.25 mM, almost complete inactivation after 8 min, NADP+ or NADPH accelerate inhibition rate
dephospho-CoA
-
competitive vs. NAD(H)
Dicyclohexylcarbodiimide
-
complete inhibition if 0.5 mol are bound to 1 mol of enzyme
diethyldicarbonate
-
inhibition is approx. 50% accelerated in the presence of NAD(H)
ethoxyformic anhydride
-
2 mM, almost complete inactivation after 6 min, NADP+ or NADPH accelerate inhibition rate
fluorosulfonyl-para-benzyladenosine
-
complete inhibition if 0.5 mol are bound to 1 mol of enzyme
formamide disulfide dihydrochloride
-
0.2 mM, 43% inhibition
glutathione
glutathione disulfide
-
strong, time dependent inhibition of thio-NADP+ reduction by NADH and acetylpyridine adenine dinucleotide reduction by NADPH, 50% inhibition after 40 min incubation in 26.7 mM glutathione disulfide, presence of NADPH accelerates inhibition 20fold
K+
-
200 mM, 50% inhibition at pH 7.9, 300 mM, 40% inhibition at pH 5.5, 95% at pH 8.5
La3+
-
0.1 mM, 50% inhibition at pH 7.0, maximal inhibition at pH 8.0
methylmethane thiosulfonate
N,N'-dicyclohexylcarbodiimide
-
0.3 mM significantly inhibits the the energy-linked NADH-NADP+ reactions, but not the nonenergy-linked NADH-NADP+ transhydrogenation
N,N'-Dicylclohexylcarbodiimide
N-(4-Azido-2-nitrophenyl)-2-aminoethylsulfonate
-
trivial name NAP-taurine, time-dependent inactivation of reconstituted enzyme after photolysis in NAP-taurine loaded vesicles, acetylpyridine adenine dinucleotide stimulates inactivation
N-(Ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline
N-ethylmaleimide
Na+
-
200 mM, 50% inhibition at pH 7.9, 300 mM, 40% inhibition at pH 5.5, 95% at pH 8.5
NADP+
NADPH
p-chloromercuribenzoate
p-Chlororomercuriphenyl sulfonate
-
-
palmitoyl CoA
specifically interferes with Nnt activity by competition with NADPH-binding, 20% residual activity at 1 mM
palmitoyl-CoA
Pentane-2,4-dione
-
inactivation of chromatophore complex, 156 mM, approx. 85% inactivation after 30 min, NADPH and NADP+ partially protect, half-maximal protection with 0.015 mM NADPH and 0.030 mM NADP+ respetively
Phenylarsine oxide
Phospholipase A
-
74% inhibition of activity in submitochondrial particles
-
Phospholipase C
-
10-20% inhibition of activity in submitochondrial particles
-
pyridoxal 5'-phosphate
-
0.8 mM, almost complete inactivation after 5 min, 0.4 mM NADP+ or NADPH protect from inactivation, inhibition can be reversed to a considerable extent by L-lysine
reduced acetylpyridine adenine dinucleotide
-
competitive vs. oxidized acetylpyridine dinucleotide, noncompetitive vs. NADPH
rotenone
-
with rotenone addition, the NADH-NADP+ activity is inhibited significantly and the remaining activity reflects the nonenergy-linked reaction
S-7-nitrobenzofuran-4-yl-3'-dephospho-CoA
-
strong inhibitor, competitive vs. NAD+ and NADPH
S-7-nitrobenzofuran-4-yl-CoA
-
strong inhibitor, competitive vs. NADPH, non-competitive vs. NAD+
S-nitrosoglutathione
100% inhibition at 3 mM or higher concentration
Sr2+
-
25 mM, 50% inhibition at pH 7.0
Tl+
-
20 mM, 50% inhibition at pH 7.9
triiodothyronine
additional information
-
NNT expression is 2.8fold downregulated in the vastus lateralis muscle of calorie restricted rhesus monkeys
-