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Literature summary for 1.6.1.2 extracted from
- Purification and partial characterization of bovine heart mitochondrial pyridine dinucleotide transhydrogenase (1978), Arch. Biochem. Biophys., 187, 180-190.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Phospholipids | approx. 2.5fold activation 120 min after sonication with mitochondrial phospholipids | Bos taurus |  |
General Stability
| General Stability | Organism |
|---|---|
| unstable, loses 30-50% activity within 48 h at 4°C, remainder of the enzyme becomes inactive after 2 weeks, complete inactivation after freezing at -20°C or -70°C overnight, enzyme is very susceptible to trypsinolysis, NADH protects from tryptic inactivation, NADPH promotes tryptic inactivation | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM, aprrox. 20% inhibition, 20 mM, approx. 60% inhibition | Bos taurus | |
| palmitoyl-CoA | competitive inhibition vs. NADPH | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial membrane | - |
Bos taurus | 31966 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
2 * 120000, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bos taurus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 120000, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 6.3 | - |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.7 | 6.7 | less than 50% of maximal activity above and below | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bos taurus | |
| NADH | - |
Bos taurus | |
| NADP+ | - |
Bos taurus | |
| NADPH | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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