EC Number |
Protein Variants |
Reference |
---|
4.2.3.19 | more |
a synthetic ent-kaurene module, expressing Stevia rebaudiana CPPS and KS, and Rhodobacter sphaeroides GGPPS (crtE gene), is constructed for ent-kaurene production in diverse Escherichia coli strains, best in strain MG1655, method optimization, several crtE genes from Rhodobacter species are validated, overview |
748267 |
4.2.3.19 | more |
construction of a SmKS-SmCPSent fusion enzyme (CPSent is ent-copalyl diphosphate synthase, EC 5.5.1.13), which improves the ent-kaurene precursor supply in transformed Saccharomyces cerevisiae strain SGH5 (BY-T20/pESC-Trp::SmKS-SmCPSent/SmKO + pESC-Leu::SmCPR1) |
746448 |
4.2.3.19 | more |
construction of chimeric proteins of Physcomitrella patens PpCPS/KS with Jungermannia subulata, the chimeric cyclases Pp131-566 /Js574-86 and Pp131-504 /Js512-886 and produce only ent-kaurene. Chimeric cyclases, Pp131-622 /Js630-886 and Pp131-714 /Js722-886, lose all enzymic activity |
714971 |
4.2.3.19 | more |
construction of chimeric proteins of Physcomitrella patens PpCPS/KS with Jungermannia subulata, the chimeric cyclases Pp131-566 /Js574-886 and Pp131-504 /Js512-886 and produce only ent-kaurene. Chimeric cyclases, Pp131-622 /Js630-886 and Pp131-714 /Js722-886, lose all enzymic activity |
714971 |
4.2.3.19 | more |
construction of transgenic Arabidopsis thaliana plants, using the Agrobacterium tumefaciens infection system, overexpressing KS leads to increased ent-kaurene production but not to an increase in bioactive gibberellins, no altered morphology or phenotype compared to wild-type plants |
666619 |
4.2.3.19 | more |
construction of transgenic rice mutants expressing mutated KS-like genes, the mutant plants show reduced enzyme activity, phenotype analysis and effects on gibberellin metabolism, overview |
660237 |
4.2.3.19 | more |
generation of a Physcomitrella patens CPS/KS (PpCPS/KS)-gene disruption mutant strain A74, comparisons of photomorphogenesis of protonema in the wild-type and the A74 enzyme mutant, phenotype, detailed overview |
749028 |
4.2.3.19 | D132A |
low activity |
492252 |
4.2.3.19 | D320A |
low activity |
492252 |
4.2.3.19 | A710M |
mutant converted geranylgeranyl diphosphate to ent-kaurene as the sole product |
714971 |