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Literature summary for 4.2.3.19 extracted from

  • Su, P.; Tong, Y.; Cheng, Q.; Hu, Y.; Zhang, M.; Yang, J.; Teng, Z.; Gao, W.; Huang, L.
    Functional characterization of ent-copalyl diphosphate synthase, kaurene synthase and kaurene oxidase in the Salvia miltiorrhiza gibberellin biosynthetic pathway (2016), Sci. Rep., 6, 23057 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.19

Cloned(Commentary)

Cloned (Comment) Organism
SmKS DNA and amino acid sequence determination and analysis of full-length enzyme from cDNA, sequence comparisons and phylogenetic analysis and tree, recombinant enzyme expression in Saccharomyces cerevisiae strain BY-T20 Salvia miltiorrhiza

Protein Variants

Protein Variants Comment Organism
additional information construction of a SmKS-SmCPSent fusion enzyme (CPSent is ent-copalyl diphosphate synthase, EC 5.5.1.13), which improves the ent-kaurene precursor supply in transformed Saccharomyces cerevisiae strain SGH5 (BY-T20/pESC-Trp::SmKS-SmCPSent/SmKO + pESC-Leu::SmCPR1) Salvia miltiorrhiza

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid the enzyme sequence contains a plastid transit peptide sequence, the first 27 N-terminal amino acids rich in serine and threonine (22%) Salvia miltiorrhiza 9536
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Salvia miltiorrhiza
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 ent-kaurene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Salvia miltiorrhiza A0A0U4CDK4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme sequence contains a plastid transit peptide sequence, which is cleaved off for enzyme maturation Salvia miltiorrhiza

Source Tissue

Source Tissue Comment Organism Textmining
hairy root
-
 Salvia miltiorrhiza
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root
-
 Salvia miltiorrhiza
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
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 Salvia miltiorrhiza ent-kaurene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
kaurene synthase
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 Salvia miltiorrhiza
SmKS
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 Salvia miltiorrhiza

General Information

General Information Comment Organism
evolution the enzyme is a monofunctional class I diTPS Salvia miltiorrhiza
metabolism the enzyme catalyzes a step in the gibberellin biosynthetic pathway. The tanshinone biosynthetic pathway is uniquely initiated by a sequential pair of cyclization reactions catalyzed by SmCPS1 and SmKSL1 to produce abietane miltiradiene. Enzyme SmCPSent catalyzes the cyclization of geranylgeranyl diphosphate to ent-copalyl diphosphate (ent-CPP), which is converted to ent-kaurene by ent-kaurene synthase, SmKS. ent-Kaurene oxidase (SmKO) then catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid Salvia miltiorrhiza
additional information the enzyme amino acid sequence contains a DDFFD motif but lacks the DxDD motif, indicating that SmKS is a plant KS protein with monofunctional class I diTPS activity Salvia miltiorrhiza