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Literature summary for 4.2.3.19 extracted from

  • Kong, M.K.; Kang, H.J.; Kim, J.H.; Oh, S.H.; Lee, P.C.
    Metabolic engineering of the Stevia rebaudiana ent-kaurene biosynthetic pathway in recombinant Escherichia coli (2015), J. Biotechnol., 214, 95-102 .
    View publication on PubMed
Search for more literature for 4.2.3.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) and MG1655 cells Stevia rebaudiana
gene KS1, a synthetic ent-kaurene module, expressing Stevia rebaudiana CPPS and KS, and Rhodobacter sphaeroides GGPPS (crtE gene), is constructed for ent-kaurene production in diverse Escherichia coli strains, best in strain MG1655 Stevia rebaudiana

Protein Variants

Protein Variants Comment Organism
additional information a synthetic ent-kaurene module, expressing Stevia rebaudiana CPPS and KS, and Rhodobacter sphaeroides GGPPS (crtE gene), is constructed for ent-kaurene production in diverse Escherichia coli strains, best in strain MG1655, method optimization, several crtE genes from Rhodobacter species are validated, overview Stevia rebaudiana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Stevia rebaudiana 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Stevia rebaudiana
-
 ent-kaurene + diphosphate
-
 ?
ent-copalyl diphosphate Stevia rebaudiana
-
 ent-kaur-ene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Stevia rebaudiana
-
-
-
Stevia rebaudiana Q9XEH9
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Stevia rebaudiana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Stevia rebaudiana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
 Stevia rebaudiana ent-kaurene + diphosphate
-
 ?
ent-copalyl diphosphate
-
 Stevia rebaudiana ent-kaur-ene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
KS1-1
-
 Stevia rebaudiana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Stevia rebaudiana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Stevia rebaudiana

General Information

General Information Comment Organism
physiological function geranylgeranyl diphosphate (GGPP) is an essential precursor in the biosynthesis of several diterpenes including ent-kaurene via formation of ent-copalyl diphosphate. Effect of isoprenoid precursors on ent-copalyl diphosphate and ent-kaurene production, ent-kaurene is quantified using GC-MS analysis Stevia rebaudiana