EC Number   |
Protein Variants |
Reference |
|---|
   3.5.1.52 | W220F |
81% of activity compared to wild type |
652230 |
| 3.5.1.52 | W231F |
83% of activity compared to wild type |
652230 |
| 3.5.1.52 | C191A |
catalytically inactive mutant enzyme |
689305 |
| 3.5.1.52 | more |
complete deletion of aPNGase activity |
-, 753034 |
| 3.5.1.52 | more |
construction of PNGase U-373MG siRNA cell lines, the glycosylated type I membrane protein class I MHC HC, dislocated by US2 protein, becomes cytosolic when peptide: N-glycanase is compromised, overview |
668402 |
| 3.5.1.52 | more |
construction of truncated enzyme variants comprising amino acid residues 1-171, 1-471, 471-651, 171-471, 1-111, 112-450, 451-651, and 1-450 |
670678 |
| 3.5.1.52 | more |
construction of truncated enzyme variants comprising amino acid residues 1-80, 35-81, 1-181, 1-181DELTAPUB, 1-130, 1-111, 112-450, and 451-651 |
670704 |
| 3.5.1.52 | more |
deletion of the N-terminal H1 helix (Png1p-DH1) enhances the deglycosylation activity of peptide:N-glycanase towards denatured glycoproteins |
713354 |
| 3.5.1.52 | more |
enzyme deletion strain, formation of free oligosaccharides is reduced by 70-80% |
649758 |
| 3.5.1.52 | more |
generation of a At3g14920 gene knockout mutant, complete deletion of aPNGase activity in F1 self plants, screened for At3g14920/At5g05480 double-knockout, from Arabidopsis Columbia T-DNA insertion mutant lines SALK_011366 (At3g14920) and SALK_018420 (At5g05480) |
-, 753034 |
