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acetylcholine receptor + H2O
?
-
-
-
-
?
alpha-dansyl derivative fetuin pentaglycopeptide + H2O
?
alpha1-antitrypsin Pi Z + H2O
deglycosylated alpha1-antitrypsin Pi Z
-
-
-
?
Antithrombin III + H2O
?
-
significant amount of only partly de-N-glycosylated protein is detected even after more than 18 h incubation time with PNGase F
-
-
?
asialo fetuin glycopeptide I + H2O
?
-
-
-
-
?
asialoglycopeptide I + H2O
?
-
fetuin-derived radio-labeled substrate, deglycosylation
-
-
?
asiolo-ovomucoid glycopeptide + H2O
?
-
-
-
-
?
bence-jones wh lambda glycopeptide + H2O
?
-
acid PNGase M from blastoderm stage 11
-
-
?
beta-aspartylglycosylamine + H2O
aspartic acid + NH3 + N-acetylglucosamine
bovine fetuin glycopeptide + H2O
?
bromelain glycopeptide + H2O
?
Prunus amygdalus var. dulcis
-
-
-
-
?
bromelain undecapeptide + H2O
?
Prunus amygdalus var. dulcis
-
-
-
-
?
coagulation factor IX + H2O
?
-
almost quantitative de-N-glycosylation with PNGase F is observed after a rather short time
-
-
?
concanavalin A-precursor + H2O
?
-
deglycosylation leads to conversion into an active lectin
-
-
?
Cys-Gly-Leu-Val-Pro-Val-Leu-Ala-Glu-Asn-Tyr-Asn(Man3Gal2GlcNAc4NeuAc2)-Lys + H2O
?
-
-
-
?
dabcyl-Gly-Glu-Asn-(GlcNAcbeta(1->2)Manalpha(1->3)[GlcNAcbeta(1->2)Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta)-Arg + H2O
GlcNAcbeta(1->2)Manalpha(1->3)[GlcNAcbeta(1->2)Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc + dabcyl-Gly-Glu-Asn-Arg
dEDANSylated hen ovomucoid glycopeptide + H2O
?
denatured ribonuclease B + H2O
?
-
-
-
-
?
denatured RNase B + H2O
?
-
SpPNGase cleaves N-glycan from denatured RNase B
-
-
?
denatured RNaseB + H2O
deglycosylated RNaseB
-
-
-
?
fetuin glycopeptide + H2O
?
fetuin glycopeptide II + H2O
?
-
acid PNGase M from blastoderm stage 11
-
-
?
fetuin-derived asialoglycopeptide I + H2O
?
-
([14C]-CH3)2Leu-Asn(GlcNAc5-Man3Gal3)-Asp-Ser-Arg
-
-
?
Gal2GlcNAc2Man3GlcNAc2-Asn-peptide + H2O
?
-
-
-
-
?
GlcNAc-Asn-peptide + H2O
?
-
-
-
-
?
glycoprotein US11 + H2O
?
-
the enzyme deglycolyzes HCMV glycoprotein US11
-
-
?
glycoprotein US2 + H2O
?
-
the enzyme deglycolyzes HCMV glycoprotein US2
-
-
?
horse radish peroxidase + H2O
?
only PNGase F-II can release alpha->1,3 core-fucosylated glycans from HRP
-
-
?
HR23-ubiquitin-like domain + H2O
?
the N-terminal domain of PNGase (PUB) interacts with HR23-ubiquitin-like domain and ubiquitin chains
-
-
?
human asialotransferrin + H2O
?
-
-
-
-
?
human IgG + H2O
?
-
-
-
-
?
human transferrin glycopeptide + H2O
?
-
-
-
?
L-hyosophorin + H2O
?
-
neutral and acid PNGase M
-
-
?
laccase + H2O
?
-
-
-
-
?
Leu-Asn(GlcNAc5Man3Gal3)-Asp-Ser-Arg + H2O
?
fetuin-derived asialoglycopeptide I, 14C, substrate activity assay
-
-
?
Man5GlcNAc2-Asn-peptide + H2O
?
-
-
-
-
?
melanopsin + H2O
?
-
-
-
?
mu opioid receptor + H2O
?
-
the enzyme removes all N-linked glycans
-
-
?
N-glycoprotein + H2O
?
-
PNGase is involved in the release of N-glycans from N-glycoproteins
-
-
?
Oryzias latipes glycophosphoprotein MU-1 + H2O
?
-
acid PNGase M from blastoderm stage 11, yolk-absorptive stage
-
-
?
Oryzias latipes glycophosphoprotein MU-2 + H2O
?
-
acid PNGase M from blastoderm stage 11, yolk-absorptive stage
-
-
?
ovalbumin + H2O
?
-
deglycosylation of the heat-denatured protein
-
-
?
ovalbumin glycopeptide + H2O
aspartic acid + NH3 + N-acetylglucosamine
ovotransferrin glycopeptide + H2O
?
Prunus amygdalus var. dulcis
-
Gly-Leu-Ile-His-Asn(oligosaccharide)-Arg
-
-
?
PHA glycopeptide + H2O
?
-
-
-
-
?
pineapple stem bromelain glycopeptide + H2O
?
porcine fibrinogen + H2O
?
-
-
-
-
?
proton-coupled folate transporter + H2O
?
-
-
-
-
?
receptor for advanced glycation end-products isoform H-300 + H2O
?
-
-
-
-
?
receptor for advanced glycation end-products isoform N-16 + H2O
?
-
-
-
-
?
ricin A + H2O
?
-
deglycosylation of ricin A, the enzyme acts in complex with protein Rad23, interaction and complex formation analysis, overview
-
-
?
RNAse + H2O
?
removes high mannose-type N-glycan from denatured RNase B, but not from native RNase B
-
-
?
RTADELTA protein + H2O
deglycosylated RTADELTA protein + ?
-
RTADELTA is a non-toxic mutant of ricin A-chain
-
-
?
RTADELTA-transmembrane-Leu2 protein + H2O
deglycosylated RTADELTA-transmembrane-Leu2 protein + ?
-
-
-
-
?
sialylglycopeptide + H2O
?
asialo-, agalactosyl-, trimannosyl- and monomanosyl-sialylglycopeptides are hydrolyzed
-
-
?
taka-amylase A + H2O
?
Prunus amygdalus var. dulcis
-
1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1
-
-
?
transferrin + H2O
?
Prunus amygdalus var. dulcis
-
desialylated, human
-
-
?
truncated RNaseB + H2O
deglycosylated RNaseB
-
-
-
?
XylMan3FucGlcNAc2-Asn-peptide from horse raddish peroxidase + H2O
?
-
-
-
-
?
yeast carboxypeptidase + H2O
?
-
deglycosylation of heat-denatured protein
-
-
?
additional information
?
-
alpha-dansyl derivative fetuin pentaglycopeptide + H2O
?
-
-
-
-
?
alpha-dansyl derivative fetuin pentaglycopeptide + H2O
?
-
-
-
-
?
alpha-dansyl derivative fetuin pentaglycopeptide + H2O
?
-
-
-
-
?
alpha-dansyl derivative fetuin pentaglycopeptide + H2O
?
Prunus amygdalus var. dulcis
-
-
-
-
?
alpha-dansyl derivative fetuin pentaglycopeptide + H2O
?
-
-
-
-
?
ATPase p97 + H2O
?
a cofactor-binding motif of p97 contained within the last 10 amino acid residues of the C terminus is both necessary and sufficient to mediate interactions of p97 with PNGase. Phosphorylation of p97s highly conserved penultimate tyrosine residue, which is the main phosphorylation site during T cell receptor stimulation, completely blocks binding of either PNGase or Ufd3 to p97. This observation suggests that phosphorylation of this residue modulates endoplasmic reticulum-associated protein degradation activity by discharging substrate-processing cofactors
-
-
?
ATPase p97 + H2O
?
a cofactor-binding motif of p97 contained within the last 10 amino acid residues of the C terminus is both necessary and sufficient to mediate interactions of p97 with PNGase
-
-
?
beta-aspartylglycosylamine + H2O
aspartic acid + NH3 + N-acetylglucosamine
-
1-L-beta-aspartamido-2-acetamido-1,2-dideoxy-beta-D-glucose
-
?
beta-aspartylglycosylamine + H2O
aspartic acid + NH3 + N-acetylglucosamine
-
1-L-beta-aspartamido-2-acetamido-1,2-dideoxy-beta-D-glucose
-
?
beta-aspartylglycosylamine + H2O
aspartic acid + NH3 + N-acetylglucosamine
-
1-L-beta-aspartamido-2-acetamido-1,2-dideoxy-beta-D-glucose
-
?
bovine fetuin glycopeptide + H2O
?
-
-
-
-
?
bovine fetuin glycopeptide + H2O
?
-
-
-
-
?
bovine fetuin glycopeptide + H2O
?
-
Leu-Ala-Asn-(oligosaccharide)-AeCys-Ser
-
-
?
bovine fetuin glycopeptide + H2O
?
-
-
-
-
?
bovine fetuin glycopeptide + H2O
?
-
Leu-Asn(Man3Gal3GlcNAc5)-Asp-Ser-Arg
-
-
?
bovine fetuin glycopeptide + H2O
?
-
-
-
-
?
class I MHC HC + H2O
?
-
the enzyme is involved proteasomal degradation of glycosylated type I membrane protein class I MHC heavy chain, dislocated from ER to cytosol by cytomegalovirus-encoded glycoprotein US2 protein, overview
-
-
?
class I MHC HC + H2O
?
-
the enzyme deglycosylates dislocated membrane protein class I MHC heavy chain
-
-
?
dabcyl-Gly-Glu-Asn-(GlcNAcbeta(1->2)Manalpha(1->3)[GlcNAcbeta(1->2)Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta)-Arg + H2O
GlcNAcbeta(1->2)Manalpha(1->3)[GlcNAcbeta(1->2)Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc + dabcyl-Gly-Glu-Asn-Arg
-
-
-
-
?
dabcyl-Gly-Glu-Asn-(GlcNAcbeta(1->2)Manalpha(1->3)[GlcNAcbeta(1->2)Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta)-Arg + H2O
GlcNAcbeta(1->2)Manalpha(1->3)[GlcNAcbeta(1->2)Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc + dabcyl-Gly-Glu-Asn-Arg
-
-
-
-
?
dEDANSylated hen ovomucoid glycopeptide + H2O
?
-
-
-
-
?
dEDANSylated hen ovomucoid glycopeptide + H2O
?
-
DGP
-
-
?
fetuin glycopeptide + H2O
?
-
-
-
-
?
fetuin glycopeptide + H2O
?
-
-
-
-
?
fetuin glycopeptide + H2O
?
Prunus amygdalus var. dulcis
-
-
-
-
?
glycopeptide + H2O
?
Prunus amygdalus var. dulcis
-
with 3-11 amino acid residues
-
-
?
glycopeptide + H2O
?
-
-
-
-
?
ovalbumin glycopeptide + H2O
aspartic acid + NH3 + N-acetylglucosamine
-
Glu-Glu-Lys-Tyr-Asn(oligosaccharide)-Leu-Thr-Ser-Val
-
-
?
ovalbumin glycopeptide + H2O
aspartic acid + NH3 + N-acetylglucosamine
Prunus amygdalus var. dulcis
-
3.5.1.52 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
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