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Literature summary for 3.5.1.52 extracted from

  • Li, G.; Zhou, X.; Zhao, G.; Schindelin, H.; Lennarz, W.J.
    Multiple modes of interaction of the deglycosylation enzyme, mouse peptide N-glycanase, with the proteasome (2005), Proc. Natl. Acad. Sci. USA, 102, 15809-15814.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of the His6-tagged wild-type enzyme and truncated variants with GST-tagged murine proteasome components HR23B, S4, and autocrine motility factor receptor AMFR in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of truncated enzyme variants comprising amino acid residues 1-171, 1-471, 471-651, 171-471, 1-111, 112-450, 451-651, and 1-450 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mus musculus 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus the enzyme mediates the binding of the cytoplasmic proteins p97 and HR23B to the proteasome through formation of a ternary complex, p97 binds the autocrine motility factor receptor AMFR, modeling of interaction of the endoplasmic reticulum with the proteasome, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged proteasome components HR23B, S4, and AMFR from Escherichia coli by glutathione affinity batch method, recombinant His6-tagged wild-type enzyme and truncated variants from Escherichia coli by nickel affinity chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
asialoglycopeptide I + H2O fetuin-derived radio-labeled substrate, deglycosylation Mus musculus ?
-
?
additional information the enzyme mediates the binding of the cytoplasmic proteins p97 and HR23B to the proteasome through formation of a ternary complex, p97 binds the autocrine motility factor receptor AMFR, modeling of interaction of the endoplasmic reticulum with the proteasome, overview Mus musculus ?
-
?
additional information the enzyme deglycosylates misfolded proteins in the cytosol and shows multiple modes of interaction with the proteasome, overview Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
peptide N-glycanase
-
Mus musculus
PGNase
-
Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Mus musculus