Cloned (Comment) | Organism |
---|---|
co-expression of His-tagged or GST-tagged truncated enzyme variants and of GST-tagged enzyme mutants with His-tagged p97, transient co-expression and complex formatin of N-terminal GFP-tagged PNGase and c-myc-tagged AMFR in COS-1 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
G79/F80A | site-directed mutagenesis, the double mutation completely abolishes the interaction of PNGase with p97 | Mus musculus |
additional information | construction of truncated enzyme variants comprising amino acid residues 1-80, 35-81, 1-181, 1-181DELTAPUB, 1-130, 1-111, 112-450, and 451-651 | Mus musculus |
N41P | site-directed mutagenesis, the mutation completely abolishes the interaction of PNGase with p97 | Mus musculus |
N58A | site-directed mutagenesis, the mutation does not affect the interaction of PNGase with p97 | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | the enzyme removes N-linked oligosaccharides from misfolded glycoproteins as part of endoplasmic reticulum-associated degradation pathway involving a complex formation with proteins HR23B, cytosolic protein Y33K, p97, and autocrine motility factor receptor AMFR, the AAA ATPase p97 links peptide N-glycanase to the endoplasmic reticulum-associated E3 ligase AMFR, the N-terminus of PNGase interacts with the C-terminal tail of AMFR, complex formation model, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme removes N-linked oligosaccharides from misfolded glycoproteins as part of endoplasmic reticulum-associated degradation pathway involving a complex formation with proteins HR23B, cytosolic protein Y33K, p97, and autocrine motility factor receptor AMFR, the AAA ATPase p97 links peptide N-glycanase to the endoplasmic reticulum-associated E3 ligase AMFR, the N-terminus of PNGase interacts with the C-terminal tail of AMFR, complex formation model, overview | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the N-terminus of PNGase interacts with the C-terminal tail of AMFR, and PNGase interacts with protein p97 essentially requiring the enzymes' PUB domain, overview, complex formation model, overview | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
peptide N-glycanase | - |
Mus musculus |
PGNase | - |
Mus musculus |