EC Number |
Protein Variants |
Reference |
---|
3.1.1.53 | F211A |
abrogated ligand recognition and binding |
700987 |
3.1.1.53 | L266A |
abrogated ligand recognition and binding |
700987 |
3.1.1.53 | L267A |
abrogated ligand recognition and binding |
700987 |
3.1.1.53 | S40A |
catalytically inactive, active site residue, retained lectin activity |
700987 |
3.1.1.53 | H377A |
complete loss of activity |
750708 |
3.1.1.53 | T132A |
complete loss of activity |
750708 |
3.1.1.53 | more |
construction of recombinant MHV-A59 derivatives in which the autologous genes for hemagglutinin-esterase and sialidase are replaced by those of MHV-S or MHV-DVIM by targeted RNA recombination. The mutants do not express functional hemagglutinin-esterase, in MHV-A59, the hemagglutinin-esterase gene is interrupted by a nonsense mutation at codon 15 and that consequently MHV-A59 and derivates rMHV-A59-SDVIM and rMHV-A59-SS do not express the hemagglutinin-esterase protein |
716066 |
3.1.1.53 | Y184A |
decreased ligand binding affinity |
700987 |
3.1.1.53 | S57T |
loss of esterase activity |
673893 |
3.1.1.53 | A385T |
naturally occuring mutation |
730718 |