Any feedback?
Literature summary for 3.1.1.53 extracted from
- Human sialic acid acetyl esterase Towards a better understanding of a puzzling enzyme (2015), Glycobiology, 25, 992-1006 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in COS-7 cells | Homo sapiens |
| expression in COS7 cell | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H377A | complete loss of activity | Homo sapiens |
| H377A | the mutation abolishes the enzymatic activity | Homo sapiens |
| S332A | no significant influence on activity | Homo sapiens |
| S332A | the mutation does not influence the activity of the enzyme significantly | Homo sapiens |
| T132A | complete loss of activity | Homo sapiens |
| T132A | the mutation abolishes the enzymatic activity | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| extracellular | - |
Homo sapiens | - |
- |
| Golgi apparatus | - |
Homo sapiens | 5794 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sialic acid + H2O | Homo sapiens | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | Q9HAT2 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | glycosylation influences the biological activity of the enzyme and is essential for release of the enzyme into the culture medium | Homo sapiens |
| glycoprotein | N-glycosylation sites: N107, N138, N267, N290, N401, N422. O-glycosylation sites: S177, S231, S242, T511 | Homo sapiens |
| phosphoprotein | phosphorylation sites: S30, Y31, T52, S74, S79, S144, S156, S158, S217, T221, S227, S228, S247, S255, T303, S308, T312, S328, S331, S332, Y349, S371, T461, S470 | Homo sapiens |
| proteolytic modification | the 58000 kDa form of SIAE is rapidly proteolitically cleaved to a 49000 Da form or glycosylated to the 62000 Da form | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| colon | high expression level | Homo sapiens | - |
| kidney | high expression level | Homo sapiens | - |
| additional information | gene is expressed in all tissues tested, although at different levels | Homo sapiens | - |
| thyroid gland | high expression level | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Homo sapiens | 4-nitrophenol + acetate | - |
? | |
| sialic acid + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE | Homo sapiens |
| ? | x * 58000, calculated from sequence, x * 62000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SIAE | - |
Homo sapiens |
| sialic acid acetyl esterase | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | 8.5 | - |
Homo sapiens |
| 8.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
negligible activity below | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
