EC Number   |
Protein Variants |
Reference  |
|---|
   3.1.1.53 | S57T |
loss of esterase activity |
673893 |
| 3.1.1.53 | F211A |
abrogated ligand recognition and binding |
700987 |
| 3.1.1.53 | L266A |
abrogated ligand recognition and binding |
700987 |
| 3.1.1.53 | L267A |
abrogated ligand recognition and binding |
700987 |
| 3.1.1.53 | S40A |
catalytically inactive, active site residue, retained lectin activity |
700987 |
| 3.1.1.53 | Y184A |
decreased ligand binding affinity |
700987 |
| 3.1.1.53 | more |
the mutant SsNeuAs truncated from 166 to 233 amino acids at the N-terminal a re active for pNP-Ac and their esterase activities are almost the same as the wild-type, only with one exception of the SsNeuA167-410, while the mutant SsNeuAs terminated at amino acid position of 227, 232, 233, 241, 247, 267, 283, 293, 312, 322, 338, 355 and 377 are inactive in CMP-Neu5Ac synthetase activity, even removal of 33 amino acid residues at C-terminal of the SsNeuA leads to a complete loss of CMP-Neu5Ac synthetase activity |
714062 |
| 3.1.1.53 | S258A |
site-directed mutagenesis |
714062 |
| 3.1.1.53 | more |
construction of recombinant MHV-A59 derivatives in which the autologous genes for hemagglutinin-esterase and sialidase are replaced by those of MHV-S or MHV-DVIM by targeted RNA recombination. The mutants do not express functional hemagglutinin-esterase, in MHV-A59, the hemagglutinin-esterase gene is interrupted by a nonsense mutation at codon 15 and that consequently MHV-A59 and derivates rMHV-A59-SDVIM and rMHV-A59-SS do not express the hemagglutinin-esterase protein |
716066 |
| 3.1.1.53 | E26A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type NanS |
716859 |
